LOCUS       V043074                10210 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043074
VERSION     V043074
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4236
                     /label="LRRC4C(NM_020929)"
                     /note="LRRC4C(NM_020929)"
                     /gene="LRRC4C"
     CDS             4243..4308
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4352..4563
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4576..4844
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4873..4882
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4903..5565
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5635..5688
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5689..6288
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6289..6877
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6951..7184
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7256..7377
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7417..7552
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7585..7601)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7609..7625
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7633..7663)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7678..7699
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7987..8575)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8746..9606)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9607..9711)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10185..10201
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tgaacaagat gaccttacat
     2341 ccacagcaga taatgatagg tcctaggttt aacagggccc tatttgaccc cctgcttgtg
     2401 gtgctgctgg ctcttcaact tcttgtggtg gctggtctgg tgcgggctca gacctgccct
     2461 tctgtgtgct cctgcagcaa ccagttcagc aaggtgattt gtgttcggaa aaacctgcgt
     2521 gaggttccgg atggcatctc caccaacaca cggctgctga acctccatga gaaccaaatc
     2581 cagatcatca aagtgaacag cttcaagcac ttgagacact tggaaatcct acagttgagt
     2641 aggaaccata tcagaaccat tgaaattggg gctttcaatg gtctggcgaa cctcaacact
     2701 ctggaactct ttgacaatcg tcttactacc atcccgaatg gagcttttgt atacttgtct
     2761 aaactgaagg agctctggtt gcgaaacaac cccattgaaa gcatcccttc ttatgctttt
     2821 aacagaattc cttctttgcg ccgactagac ttaggggaat tgaaaagact ttcatacatc
     2881 tcagaaggtg cctttgaagg tctgtccaac ttgaggtatt tgaaccttgc catgtgcaac
     2941 cttcgggaaa tccctaacct cacaccgctc ataaaactag atgagctgga tctttctggg
     3001 aatcatttat ctgccatcag gcctggctct ttccagggtt tgatgcacct tcaaaaactg
     3061 tggatgatac agtcccagat tcaagtgatt gaacggaatg cctttgacaa ccttcagtca
     3121 ctagtggaga tcaacctggc acacaataat ctaacattac tgcctcatga cctcttcact
     3181 cccttgcatc atctagagcg gatacattta catcacaacc cttggaactg taactgtgac
     3241 atactgtggc tcagctggtg gataaaagac atggccccct cgaacacagc ttgttgtgcc
     3301 cggtgtaaca ctcctcccaa tctaaagggg aggtacattg gagagctcga ccagaattac
     3361 ttcacatgct atgctccggt gattgtggag ccccctgcag acctcaatgt cactgaaggc
     3421 atggcagctg agctgaaatg tcgggcctcc acatccctga catctgtatc ttggattact
     3481 ccaaatggaa cagtcatgac acatggggcg tacaaagtgc ggatagctgt gctcagtgat
     3541 ggtacgttaa atttcacaaa tgtaactgtg caagatacag gcatgtacac atgtatggtg
     3601 agtaattccg ttgggaatac tactgcttca gccaccctga atgttactgc agcaaccact
     3661 actcctttct cttacttttc aaccgtcaca gtagagacta tggaaccgtc tcaggatgag
     3721 gcacggacca cagataacaa tgtgggtccc actccagtgg tcgactggga gaccaccaat
     3781 gtgaccacct ctctcacacc acagagcaca aggtcgacag agaaaacctt caccatccca
     3841 gtgactgata taaacagtgg gatcccagga attgatgagg tcatgaagac taccaaaatc
     3901 atcattgggt gttttgtggc catcacactc atggctgcag tgatgctggt cattttctac
     3961 aagatgagga agcagcacca tcggcaaaac catcacgccc caacaaggac tgttgaaatt
     4021 attaatgtgg atgatgagat tacgggagac acacccatgg aaagccacct gcccatgcct
     4081 gctatcgagc atgagcacct aaatcactat aactcataca aatctccctt caaccacaca
     4141 acaacagtta acacaataaa ttcaatacac agttcagtgc atgaaccgtt attgatccga
     4201 atgaactcta aagacaatgt acaagagact caaatcggat ccgactacaa agaccatgac
     4261 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4321 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4381 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4441 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4501 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4561 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4621 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4681 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4741 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4801 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     4861 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     4921 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     4981 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5041 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5101 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5161 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5221 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5281 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5341 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5401 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5461 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     5521 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     5581 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     5641 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     5701 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5761 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5821 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5881 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5941 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6001 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6061 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6121 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6181 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6241 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6301 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6361 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6421 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6481 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6541 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6601 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6661 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6721 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6781 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6841 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     6901 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6961 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7021 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7081 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7141 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7201 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7261 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7381 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7501 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     7561 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     7621 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7681 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7741 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7981 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8161 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8401 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8461 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8641 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8701 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8761 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8821 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8881 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8941 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9001 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9061 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9121 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9181 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9241 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9301 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9361 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9421 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9481 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9541 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9601 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9661 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9721 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     9781 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9841 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9901 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9961 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10021 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10081 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10141 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10201 tgccaagctg
//
