LOCUS       V046874                10124 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046874
VERSION     V046874
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        131..334
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             417..2426
                     /label="LRRC45(NM_144999)"
                     /note="LRRC45(NM_144999)"
                     /gene="LRRC45"
     CDS             2457..3167
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    3207..3324
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        3373..3883
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     CDS             3905..4504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     LTR             4632..4865
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    4937..5058
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      5098..5233
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(5254..5272)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(5282..5298)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      5440..5895
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5921..6025
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6026..6886
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7057..7645
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    7933..7954
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        7969..7999
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8007..8023
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     8031..8047
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        8068..8086
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        8114..8340
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             8341..8521
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    8568..8693
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    9186..9419
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             9604..9648
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac gccaccatgg
      421 aggagttccg gcgctcctac agccgcctgt gcagggagag tggggccgag ccccaggagg
      481 ctgtcctgca gcagctgcac cagcttccca ggggccggct ggacctggcc acgcaaagcc
      541 tgacggtgga gacctgcagg gccctgggca agctgctgcc gagggagacg ctgtgcacgg
      601 agctggtcct gagtgactgc atgctcagcg aggaaggggc cacactgctg ctccgaggcc
      661 tgtgtgccaa caccgtgctg cgctttctgg acttaaaggg caacaacctt cgggctgcag
      721 gggccgaggc tctgggaaaa ctcctccaac agaacaagtc cattcagagc ctcacgctgg
      781 agtggaacag cctgggcacg tgggacgatg ccttcgccac cttctgcggg ggcctggcgg
      841 ccaacggcgc cctgcagcgg ctggacctcc gcaacaacca gatcagtcac aagggcgcgg
      901 aggagctggc cctagccctg aagggcaaca ccaccctcca gcagctggac ctgcgctgga
      961 ataacgttgg cctcctgggg ggccgggccc tcatgaactg tctccccagc aacagaaccc
     1021 tgtggagact ggacctggct gggaacaaca tccctggaga cgtcctcaga gccgtggagc
     1081 aagccatggg ccacagccag gaccggctca ccaccttcca ggagaaccaa gcccgcactc
     1141 acgtcctcag caaggaggtc cagcacctcc gggaggagaa gtccaagcag ttcctcgact
     1201 tgatggagac tattgataag cagcgagaag agatggccaa gagcagcagg gcgtcggcag
     1261 cccgtgtagg gcagcttcag gaagccctga atgagaggca ctccatcatc aacgctctca
     1321 aggccaagct gcagatgaca gaggccgccc tggctctgtc ggagcagaag gcccaggacc
     1381 tgggggagct cctggccaca gcggagcagg agcagctgag cctgtcacag aggcaggcca
     1441 aggagctcaa gctggagcag caggaagctg cagagcggga gtctaaactc ctcagagact
     1501 tgtctgctgc caatgaaaag aacctgcttc tgcaaaacca ggtagacgag ttggagcgga
     1561 agttcaggtg tcagcaggag cagctgttcc agaccaggca ggagatgacc agcatgtcag
     1621 ctgagctgaa gatgcgggcc atccaggccg aggagcgcct ggacatggag aagagaagat
     1681 gcagacagag cctggaggac tccgaaagcc tgcgcatcaa ggaggtggag catatgaccc
     1741 gtcacctgga ggagagtgag aaggccatgc aggagcgggt gcagaggctg gaggcggcgc
     1801 ggctgtccct ggaggaggag ctgagccgag tgaaagcagc ggcactcagc gagcgtggcc
     1861 aggctgagga ggagctgatc aaggccaaga gccaggcccg cctggaggag caacagcgcc
     1921 tggctcacct ggaggacaag ctgagactgc tggcgcaggc acgggacgag gcgcagggcg
     1981 cttgcctaca gcagaagcag gtggtggccg aggcccagac ccgggtcagc cagctgggcc
     2041 tgcaagttga gggcctgcgg cggcgcctgg aagagctgca gcaggagctg agcctcaagg
     2101 accaggaaag ggtggccgag gtgagcaggg tgcgcgtgga gctgcaggag cagaacggcc
     2161 ggctgcaggc ggagctggcg gctcaggagg cgctgaggga gaaggcggcg gccctggagc
     2221 gccagctgaa agtgatggcg agcgaccacc gagaggcgct gctggacagg gagagcgaga
     2281 acgcgtctct ccgggagaag ctgcggctcc gggaggcgga gatcgcccgc atccgggacg
     2341 aggaggccca gagggcgagc ttcctgcaga acgccgtcct ggcttacgtg caggcgtccc
     2401 ccgtgaggac cctgagcccc ccaaagggtg gaggcggatc aggaggcggt ggatctatgg
     2461 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     2521 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2581 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     2641 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     2701 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2761 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2821 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     2881 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     2941 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     3001 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     3061 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     3121 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgaatt ctcgacctcg
     3181 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3241 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3301 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     3361 ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     3421 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     3481 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     3541 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     3601 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     3661 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     3721 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     3781 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     3841 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct
     3901 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3961 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     4021 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4081 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4141 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4201 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4261 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4321 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4381 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4441 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4501 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     4561 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4621 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     4681 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4741 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4801 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4861 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4921 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4981 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5041 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5101 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5161 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5221 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     5281 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5341 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5401 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     5461 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5521 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5581 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5641 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5701 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5761 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5821 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5881 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5941 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6001 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6061 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6121 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6181 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6241 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6301 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6361 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6421 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6481 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6541 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6601 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6661 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6721 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6781 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6841 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6901 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6961 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7021 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7081 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7141 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7201 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7261 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7321 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7381 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7441 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7501 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7561 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7621 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7681 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7741 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7801 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7861 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     7921 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7981 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8041 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     8101 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     8161 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     8221 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     8281 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     8341 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8401 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8461 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8521 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     8581 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     8641 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     8701 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     8761 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     8821 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     8881 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     8941 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9001 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     9061 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9121 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9181 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9241 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9301 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9361 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9421 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9481 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9541 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9601 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9661 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9721 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9781 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     9841 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     9901 cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc
     9961 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
    10021 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
    10081 gggtagtttg cagttttaaa attatgtttt aaaatggact atca
//
