LOCUS       V040860                10726 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040860
VERSION     V040860
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4752
                     /label="LRRC41(NM_006369)"
                     /note="LRRC41(NM_006369)"
                     /gene="LRRC41"
     CDS             4759..4824
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4868..5079
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5092..5360
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5389..5398
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5419..6081
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6151..6204
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6205..6804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6805..7393
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7467..7700
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7772..7893
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7933..8068
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8101..8117)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8125..8141
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8149..8179)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8194..8215
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8503..9091)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9262..10122)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10123..10227)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10701..10717
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcgcccga ggcctggcgc
     2341 gcccggagtt gctggttctg tgaggtagcg gcggcaacga ccatggaggc cacgtcccgg
     2401 gaggcggcgc cagcgaagag ctcggcctcg ggccccaacg ctccccccgc cctgttcgag
     2461 ctgtgcgggc gggcggtgag cgcccatatg ggggttctgg agagcggggt gtgggccctc
     2521 ccaggcccaa tacttcaaag catcctacct ctgctcaata tatattactt ggagaggatt
     2581 gaggaaactg ccctcaagaa aggcctctca actcaggcca tctggcgccg actctgggat
     2641 gaactgatga agacaaggcc ttccagtttg gaaagtgtga catgttggcg agccaagttt
     2701 atggaggcct ttttttccca tgttctacgt gggaccattg atgtgtcttc tgacaggcgt
     2761 ctttgtgatc agcggttctc acctcttctg cacagctccc gccatgtccg acagctcacc
     2821 atctgtaaca tgctgcaggg tgcaaccgag ctggtggctg agcccaaccg cagggttctg
     2881 gagaccctgg ccagctccct gcacactctc aagttccgcc acctgctgtt ctctgatgtg
     2941 gctgctcagc agtcacttcg gcagctgttg catcagctca ttcaccatgg ggctgtcagt
     3001 caagtgtcgc tatactcctg gcctgtgcct gagtcagccc ttttcatcct tattctcacc
     3061 atgagtgctg gcttctggca accagggcct ggtggcccac cctgccgcct ctgtggagag
     3121 gcctcccgag gccgggcccc atcccgagat gaagggtccc tcttattggg ctcacgtcgg
     3181 ccccgccggg atgctgctga gcgatgtgct gcagccctga tggccagccg gcgtaagagt
     3241 gaagccaagc agatgcccag agctgcacct gccactcggg taacacgccg gagcacacag
     3301 gagagcctga cagcaggcgg aacagacctt aagagggagc tgcacccccc agccacctcc
     3361 catgaggctc ctggcaccaa gcggtcacct tctgctccag cagccacctc ctctgcctct
     3421 tcttctacat cctcatacaa acgggcacca gctagctcag ccccacagcc taagccccta
     3481 aagcgtttca agcgagctgc agggaagaag ggtgctcgca cccgtcaggg gcctggtgca
     3541 gagtctgaag acctgtatga cttcgttttt attgtggctg gcgagaagga ggatggcgaa
     3601 gagatggaga ttggggaagt ggcttgtgga gctttggatg gatcagatcc cagctgcctg
     3661 gggcttccag cactggaagc ttcacaaaga ttccgcagca tctccacctt ggagctattc
     3721 acagttccac tctccacaga ggcagccctg acactatgcc acctgctgag ctcctgggtg
     3781 tcactggaga gcctcacact ctcctacaat ggcctgggct ctaacatctt ccgcctgcta
     3841 gacagcctgc gggccctgtc aggccaggct ggatgtcgcc tccgtgccct gcatctcagt
     3901 gacctgttct caccactgcc catcctggag ctgacacgtg ctatcgtgcg agcactgccc
     3961 ctgctacggg tcctctctat tcgtgttgac cacccaagcc agcgggacaa ccctggtgtg
     4021 ccagggaatg cagggccccc tagccacata ataggcgatg aggagatacc agaaaactgc
     4081 ctggagcagt tggagatggg atttccacgg ggagcccagc cagccccact gctgtgctcc
     4141 gttctgaagg cctcgggttc tctgcagcag ctgtccctgg atagtgccac ctttgcctct
     4201 ccccaggatt ttgggcttgt tttgcaaaca ctcaaagagt acaacctagc cctgaaaaga
     4261 ctgagcttcc atgacatgaa tctcgctgac tgtcagagcg aggtgctctt tttgctacag
     4321 aatctgactc tgcaagagat taccttctcc ttctgccgtc tgtttgagaa gcgcccagcc
     4381 caatttctgc ctgagatggt tgctgctatg aagggcaact ccacactgaa gggcctccgg
     4441 ctgccaggga accgcctggg gaatgctggc ctgctggcct tggcagatgt tttctcagag
     4501 gattcatcct cctctctctg tcagctggac atcagttcca actgcatcaa gccagatggg
     4561 cttctggagt tcgccaagcg gctggagcgc tggggccgtg gagcctttgg tcacctgcgc
     4621 ctcttccaaa actggctgga ccaggatgca gtcacagcca gggaagccat ccggcggctc
     4681 cgggctacct gccatgtggt tagcgactca tgggactcat cccaggcctt cgcagattat
     4741 gttagcacca tgggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     4801 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     4861 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4921 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4981 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5041 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5101 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     5161 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     5221 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5281 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5341 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     5401 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     5461 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     5521 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     5581 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     5641 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     5701 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     5761 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     5821 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     5881 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     5941 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     6001 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     6061 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     6121 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     6181 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     6241 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6301 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6361 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6421 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6481 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6541 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6601 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6661 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6721 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6781 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     6841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7201 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7381 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7441 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     7501 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7561 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7621 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7681 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7741 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8041 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     8101 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8161 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8221 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8281 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8701 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8761 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8821 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8881 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8941 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9001 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9061 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9241 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9301 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9361 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9421 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9481 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9541 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9601 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9661 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9721 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9781 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9841 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9901 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9961 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10021 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10081 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10261 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10321 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10381 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10441 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10501 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10561 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10621 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10681 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
