LOCUS       V043509                13129 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043509
VERSION     V043509
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7155
                     /label="LRP6(NM_002336)"
                     /note="LRP6(NM_002336)"
                     /gene="LRP6"
     CDS             7162..7227
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7271..7482
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7495..7763
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7792..7801
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7822..8484
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8554..8607
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8608..9207
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9208..9796
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9870..10103
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10175..10296
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10336..10471
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10504..10520)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10528..10544
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10552..10582)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10597..10618
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10906..11494)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11665..12525)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12526..12630)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13104..13120
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gggccgtcct gaggagcctc
     2341 ctggcctgca gcttctgtgt gctcctgaga gcggcccctt tgttgcttta tgcaaacaga
     2401 cgggacttgc gattggttga tgctacaaat ggcaaagaga atgctacgat tgtagttgga
     2461 ggcttggagg atgcagctgc ggtggacttt gtgtttagtc atggcttgat atactggagt
     2521 gatgtcagcg aagaagccat taaacgaaca gaatttaaca aaactgagag tgtgcagaat
     2581 gttgttgttt ctggattatt gtcccccgat gggctggcat gtgattggct tggagaaaaa
     2641 ttgtactgga cagattctga aactaatcgg attgaagttt ctaatttaga tggatcttta
     2701 cgaaaagttt tattttggca agagttggat caacccagag ctattgcctt agatccttca
     2761 agtgggttca tgtactggac agactgggga gaagtgccaa agatagaacg tgctggaatg
     2821 gatggttcaa gtcgcttcat tataataaac agtgaaattt actggccaaa tggactgact
     2881 ttggattatg aagaacaaaa gctttattgg gcagatgcaa aacttaattt catccacaaa
     2941 tcaaatctgg atggaacaaa tcggcaggca gtggttaaag gttcccttcc acatcctttt
     3001 gccttgacgt tatttgagga catattgtac tggactgact ggagcacaca ctccattttg
     3061 gcttgcaaca agtatactgg tgagggtctg cgtgaaatcc attctgacat cttctctccc
     3121 atggatatac atgccttcag ccaacagagg cagccaaatg ccacaaatcc atgtggaatt
     3181 gacaatgggg gttgttccca tttgtgtttg atgtctccag tcaagccttt ttatcagtgt
     3241 gcttgcccca ctggggtcaa actcctggag aatggaaaaa cctgcaaaga tggtgccaca
     3301 gaattattgc ttttagctcg aaggacagac ttgagacgca tttctttgga tacaccagat
     3361 tttacagaca ttgttctgca gttagaagac atccgtcatg ccattgccat agattacgat
     3421 cctgtggaag gctacatcta ctggactgat gatgaagtga gggccatacg ccgttcattt
     3481 atagatggat ctggcagtca gtttgtggtc actgctcaaa ttgcccatcc tgatggtatt
     3541 gctgtggact gggttgcacg aaatctttat tggacagaca ctggcactga tcgaatagaa
     3601 gtgacaaggc tcaatgggac catgaggaag atcttgattt cagaggactt agaggaaccc
     3661 cgggctattg tgttagatcc catggttggg tacatgtatt ggactgactg gggagaaatt
     3721 ccgaaaattg agcgagcagc tctggatggt tctgaccgtg tagtattggt taacacttct
     3781 cttggttggc caaatggttt agccttggat tatgatgaag gcaaaatata ctggggagat
     3841 gccaaaacag acaagattga ggttatgaat actgatggca ctgggagacg agtactagtg
     3901 gaagacaaaa ttcctcacat atttggattt actttgttgg gtgactatgt ttactggact
     3961 gactggcaga ggcgtagcat tgaaagagtt cataaacgaa gtgcagagag ggaagtgatc
     4021 atagatcagc tgcctgacct catgggccta aaggctacaa atgttcatcg agtgattggt
     4081 tccaacccct gtgctgagga aaacggggga tgtagccatc tctgcctcta tagacctcag
     4141 ggccttcgct gtgcttgccc tattggcttt gaactcatca gtgacatgaa gacctgcatt
     4201 gtcccagagg ctttcctttt gttttcacgg agagcagata tcagacgaat ttctctggaa
     4261 acaaacaata ataatgtggc tattccactc actggtgtca aagaagcttc tgctttggat
     4321 tttgatgtga cagacaaccg aatttattgg actgatatat cactcaagac catcagcaga
     4381 gcctttatga atggcagtgc actggaacat gtggtagaat tcggcttaga ttatccagaa
     4441 ggcatggcag tagactggct tgggaagaac ttgtactggg cagacacagg aacgaatcga
     4501 attgaggtgt caaagttgga tgggcagcac cgacaagttt tggtgtggaa agacctagat
     4561 agtcccagag ctctcgcgtt ggaccctgcc gaaggattta tgtattggac tgaatggggt
     4621 ggaaaaccta agatagacag agctgcaatg gatggaagtg aacgtactac cttagttcca
     4681 aatgtggggc gggcaaacgg cctaactatt gattatgcta aaaggaggct ttattggaca
     4741 gacctggaca ccaacttaat agaatcttca aatatgcttg ggctcaaccg tgaagttata
     4801 gcagatgact tgcctcatcc ttttggctta actcagtacc aagattatat ctactggacg
     4861 gactggagcc gacgcagcat tgagcgtgcc aacaaaacca gtggccaaaa ccgcaccatc
     4921 attcagggcc atttggatta tgtgatggac atcctcgtct ttcactcatc tcgacagtca
     4981 gggtggaatg aatgtgcttc cagcaatggg cactgctccc acctctgctt ggctgtgcca
     5041 gttgggggtt ttgtttgtgg atgccctgcc cactactctc ttaatgctga caacaggact
     5101 tgtagtgctc ctacgacttt cctgctcttc agtcaaaaga gtgccatcaa ccgcatggtg
     5161 attgatgaac aacagagccc cgacatcatc cttcccatcc acagccttcg gaatgtccgg
     5221 gccattgact atgacccact ggacaagcaa ctctattgga ttgactcacg acaaaacatg
     5281 atccgaaagg cacaagaaga tggcagccag ggctttactg tggttgtgag ctcagttccg
     5341 agtcagaacc tggaaataca accctatgac ctcagcattg atatttacag ccgctacatc
     5401 tactggactt gtgaggctac caatgtcatt aatgtgacaa gattagatgg gagatcagtt
     5461 ggagtggtgc tgaaaggcga gcaggacaga cctcgagccg ttgtggtaaa cccagagaaa
     5521 gggtatatgt attttaccaa tcttcaggaa aggtctccta aaattgaacg ggctgctttg
     5581 gatgggacag aacgggaggt cctctttttc agtggcttaa gtaaaccaat tgctttagcc
     5641 cttgatagca ggctgggcaa gctcttttgg gctgattcag atctccggcg aattgaaagc
     5701 agtgatctct caggtgctaa ccggatagta ttagaagact ccaatatctt gcagcctgtg
     5761 ggacttactg tgtttgaaaa ctggctctat tggattgata aacagcagca aatgattgaa
     5821 aaaattgaca tgacaggtcg agagggtaga accaaagtcc aagctcgaat tgcccagctt
     5881 agtgacattc atgcagtaaa ggagctgaac cttcaagaat acagacagca cccttgtgct
     5941 caggataatg gtggctgttc acatatttgt cttgtaaagg gggatggtac tacaaggtgt
     6001 tcttgcccca tgcacctggt tctacttcaa gatgagctat catgtggaga acctccaaca
     6061 tgttctcctc agcagtttac ttgtttcacg ggggaaattg actgtatccc tgtggcttgg
     6121 cggtgcgatg ggtttactga atgtgaagac cacagtgatg aactcaattg tcctgtatgc
     6181 tcagagtccc agttccagtg tgccagtggg cagtgtattg atggtgccct ccgatgcaat
     6241 ggagatgcaa actgccagga caaatcagat gagaagaact gtgaagtgct ttgtttaatt
     6301 gatcagttcc gctgtgccaa tggtcagtgc attggaaagc acaagaagtg tgatcataat
     6361 gtggattgca gtgacaagtc agatgaactg gattgttatc cgactgaaga accagcacca
     6421 caggccacca atacagttgg ttctgttatt ggcgtaattg tcaccatttt tgtgtctgga
     6481 actgtatact ttatctgcca gaggatgttg tgtccacgta tgaagggaga tggggaaact
     6541 atgactaatg actatgtagt tcatggacca gcttctgtgc ctcttggtta tgtgccacac
     6601 ccaagttctt tgtcaggatc tcttccagga atgtctcgag gtaaatcaat gatcagctcc
     6661 ctcagtatca tggggggaag cagtggaccc ccctatgacc gagcccatgt tacaggagca
     6721 tcatcaagta gttcttcaag caccaaaggc acttacttcc ctgcaatttt gaaccctcca
     6781 ccatccccag ccacagagcg atcacattac actatggaat ttggatattc ttcaaacagt
     6841 ccttccactc ataggtcata cagctacagg ccatatagct accggcactt tgcacccccc
     6901 accacaccct gcagcacaga tgtttgtgac agtgactatg ctcctagtcg gagaatgacc
     6961 tcagtggcaa cagccaaggg ctataccagt gacttgaact atgattcaga acctgtgccc
     7021 ccacctccca caccccgaag ccaatacttg tcagcagagg agaactatga aagctgccca
     7081 ccttctccat acacagagag gagctattct catcacctct acccaccgcc accctctccc
     7141 tgtacagact cctccggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     7201 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     7261 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     7321 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     7381 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     7441 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     7501 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     7561 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     7621 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     7681 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     7741 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     7801 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     7861 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     7921 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     7981 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     8041 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     8101 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     8161 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     8221 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     8281 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     8341 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     8401 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     8461 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     8521 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     8581 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     8641 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8701 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8761 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8821 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8881 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8941 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     9001 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     9061 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     9121 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     9181 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     9241 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     9301 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9361 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9421 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9481 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9541 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9601 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9661 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9721 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9781 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     9841 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     9901 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10081 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
    10141 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
    10201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
    10321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    10441 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
    10501 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10561 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10621 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10681 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    12001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    12061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12661 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    12721 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    12781 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    12841 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    12901 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    12961 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    13021 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    13081 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
