LOCUS       V024634                11544 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024634
VERSION     V024634
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2291..2300
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2417..2488
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2678..5563
                     /label="LRIG2(NM_001312686)"
                     /note="LRIG2(NM_001312686)"
                     /gene="LRIG2"
     CDS             5570..5659
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5703..5914
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5927..6195
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6224..6233
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6254..6916
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6986..7039
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7040..7639
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7640..8227
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8301..8534
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8606..8727
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8746..8881
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8919..8935)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8943..8959
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8967..8997)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9012..9033
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9321..9909)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10080..10940)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10941..11045)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11519..11535
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg cgccggcgcc cctaggcgtc ccggaggagc agttgctggg
     2341 gtgtcgatct agagtgcttt ctcggttact cttcattgcc cagaccgctc tcctcctgtt
     2401 gcccgccgcc ggagcagatt acaaggatga cgacgataag gactataagg acgatgatga
     2461 caaggactac aaagatgatg acgataaagg tctctgcccc gcgccctgct cctgccgcat
     2521 tcctctcctg gactgcagtc gcaggaaatt gcccgcaccg agctggaggg cgctgtcggg
     2581 cttgctgccc cccgacaccg ctatcctgga tttcagtcat aatcggttgt ctaactggaa
     2641 catcagcttg gaatcacaaa cattacagga agtgaaaatg aattacaatg aactaacaga
     2701 aatcccgtat tttggagaac ctacatctaa tattactcta ctttcattag tccataatat
     2761 aatcccagaa ataaatgcac aggcactcca gttttaccct gctctggaga gtttagacct
     2821 cagctcaaat ataatatcag aaatcaagac atcttcattt cctcgcatgc agcttaaata
     2881 cctgaattta agtaataaca gaataaccac cttggaggct ggttgcttcg ataatttatc
     2941 aagttcctta ttagtggtaa agttaaaccg taaccgaatg agcatgattc cacctaagat
     3001 cttcaagctg cctcacctcc aattcttgga acttaaaaga aacagaatta aaattgtgga
     3061 aggtcttaca ttccaagggc ttgactcctt aagatctttg aaaatgcagc ggaatggaat
     3121 tagcaaactt aaggatggag cattttttgg cttgaataac atggaagaac tagaactgga
     3181 acacaacaac cttacacgag taaacaaggg gtggttgtat ggcttgcgaa tgttacagca
     3241 gctctatgtg agccagaatg ctattgaaag aatcagccct gatgcatggg agttctgcca
     3301 aagactatcc gaacttgatt tgtcctataa ccagctgacc cgcctggatg aatctgcctt
     3361 tgtgggtctg agcttattgg agagattgaa tttaggagac aacagagtca ctcatattgc
     3421 tgatggtgta tttagatttc tttccaatct tcagacatta gacttaagaa acaatgaaat
     3481 ttcatgggcc atagaagatg ctagtgaagc ctttgctgga ctcacaagtc tcactaaact
     3541 aatcttacaa ggaaaccaga ttaagtcaat tacaaagaaa gcattcattg gtcttgaatc
     3601 ccttgagcat ctagatttga acaataatgc tataatgtct atccaagaaa atgctttttc
     3661 tcagactcat ttaaaagaac tgattctgaa cacaagcagt ttgctctgtg actgccattt
     3721 gaagtggcta cttcaatggt tggttgataa taactttcaa cattctgtga atgtaagctg
     3781 tgcacaccct gaatggctag cagggcaaag catcctgaat gtggatctga aagattttgt
     3841 ctgtgatgat tttctcaagc cacagataag gacacatcct gaaaccataa ttgctctaag
     3901 aggcatgaat gtgactctga cgtgcactgc agtgagcagc agtgattcac ccatgtccac
     3961 tgtgtggcgc aaagacagtg aaatcctgta tgacgtggat actgagaatt ttgttcgtta
     4021 ttggcagcaa gctggagaag ctctggaata tactagtatc ttacatcttt tcaatgtgaa
     4081 tttcacagat gaaggaaaat atcagtgtat tgttactaat cactttggtt ctaattattc
     4141 tcagaaagcc aaactgactg taaatgagat gccatctttt ctgaaaacgc caatggatct
     4201 gactattcgc actggtgcca tggccagatt agaatgtgct gcagagggac accctgcacc
     4261 tcagatttcc tggcagaaag atggtggtac tgactttcct gcggctcgag aaagacgcat
     4321 gcacgtcatg cccgaggatg acgtcttctt tattgccaat gtgaaaatag aagatatggg
     4381 aatctatagc tgcatggcac aaaatacagc aggaggtctc tcagcaaatg cttccctaac
     4441 agtgttagag acaccctcat ttattagacc cctggaggat aagacagtaa cacgaggtga
     4501 aactgcggtg ttacagtgca tagctggagg gagtcctgcc cctcgtctca actggactaa
     4561 agatgatggg cctttgctgg tgacagaacg acatttcttt gctgcagcca atcagcttct
     4621 catcattgta gatgccgggc tagaagatgc tgggaaatat acctgcatta tgtctaacac
     4681 ccttgggaca gaacgtggcc acatttacct aaatgtcatt tcatccccca attgtgactc
     4741 ttcccagagt agcattgggc atgaagatga tggctggacc acagttggca ttgtcatcat
     4801 tgttgtggtc tgctgtgttg ttggcacttc tttgatctgg gtcattgtta tttaccacat
     4861 gagaaggaaa aatgaagact atagtatcac aaacacagag gagctcaatc tgcctgcaga
     4921 cattcccagc tacttgtctt cccaaggaac gctgtctgag ccacaggaag gctacagcaa
     4981 ctctgaggca ggcagtcatc agcaacttat gcctcctgcc aatggatata tacacaaagg
     5041 cactgacggt ggcactggta cccgggtgat ttgctcagat tgttatgaca atgccaacat
     5101 ctactccagg acccgagaat actgtccata cacctatatt gctgaggagg acgttcttga
     5161 tcagacactg tccagcctca tggtccaaat gcctaaagag acatatttag tacatcctcc
     5221 ccaggatact actgccctag agagcctgat accgtcagcc aacagagagc catctgcctt
     5281 tcccaccaac catgagagga taagtgagaa gaaacttccc tccacacaga tgagcggtga
     5341 aacattgcag cggcccgtgt ggaacataaa cagagaacta ggcctgcctc atcctccttt
     5401 ttcccagcag ccagtccatg agtcaccaca acttcatcaa aatgagggcc tggcagggag
     5461 agagccagac tgttctgctt cttccatgtc ctgccacagg ttacaggatc atgcttttga
     5521 ttttagtagg actcggaaca ttcaagatgg tagtgagggc acaggatccg aacaaaaact
     5581 aatatcagag gaagatttgg aacagaaact aatcagtgaa gaagatttag agcaaaagtt
     5641 aatttccgag gaggacttat agcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     5701 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5761 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5821 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5881 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     5941 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     6001 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     6061 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     6121 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     6181 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga
     6241 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt
     6301 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa
     6361 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg
     6421 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc
     6481 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct
     6541 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt
     6601 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc
     6661 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac
     6721 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa
     6781 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt
     6841 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac
     6901 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc
     6961 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt
     7021 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     7081 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     7141 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     7201 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     7261 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     7321 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     7381 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7441 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7501 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7561 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7621 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7681 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7741 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7801 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7861 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7921 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7981 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     8041 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8101 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8161 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc
     8221 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8281 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     8341 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8521 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8581 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8641 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8701 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8821 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8881 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     8941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9721 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9901 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9961 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10021 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10081 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10141 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10321 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10381 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10441 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10501 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10621 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10681 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10741 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10801 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10861 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10981 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11041 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    11101 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    11161 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    11221 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    11281 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    11341 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    11401 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    11461 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    11521 aaaacgacgg ccagtgccaa gctg
//
