LOCUS       V027192                10657 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027192
VERSION     V027192
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4683
                     /label="LRFN2(NM_020737)"
                     /note="LRFN2(NM_020737)"
                     /gene="LRFN2"
     CDS             4690..4755
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4799..5010
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5023..5291
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5320..5329
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5350..6012
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6082..6135
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6136..6735
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6736..7324
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7398..7631
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7703..7824
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7864..7999
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8032..8048)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8056..8072
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8080..8110)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8125..8146
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8434..9022)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9193..10053)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10054..10158)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10632..10648
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agaccctgct tggtggcctg
     2341 ctagcgtttg gcatggcgtt tgccgtggtc gacgcctgcc ccaagtactg tgtctgccag
     2401 aatctgtctg agtcactggg gaccctgtgc ccctccaagg ggctgctctt tgtaccccct
     2461 gatattgacc ggcggacagt ggagctgcgc ctgggcggca acttcatcat ccacatcagc
     2521 cgccaggact ttgccaacat gacggggctg gtggacctga ccctgtccag gaacaccatc
     2581 agccacatcc agcccttttc ctttctggac ctcgagagcc tccgctccct gcatcttgac
     2641 agcaatcggc tgccaagcct tggggaggac accctccggg gcctggtcaa cctgcagcac
     2701 cttatcgtga acaacaacca gctgggcggc atcgcagatg aggcttttga ggacttcctg
     2761 ctgacattgg aggatctgga cctctcctac aacaacctcc atggcctgcc gtgggactcc
     2821 gtgcgacgca tggtcaacct ccaccagctg agcctggacc acaacctgct ggatcacatc
     2881 gccgagggca cctttgcaga cctgcagaaa ctggcccgcc tggatctcac ctccaatcgg
     2941 ctgcagaagc tgccccctga tcccatcttt gcccgctccc aggcttcggc tttgacagcc
     3001 acaccctttg ccccaccctt gtcctttagt tttgggggta acccacttca ctgcaattgt
     3061 gagcttctct ggctgcggag gctcgagcgg gacgatgacc tggaaacctg tggctcccca
     3121 gggggcctca agggtcgcta cttctggcat gtgcgtgagg aggagtttgt gtgcgagccg
     3181 cctctcatca cccagcacac acacaagttg ctggttctgg agggccaggc ggccacactc
     3241 aagtgcaaag ccattgggga ccccagcccc cttatccact gggtagcccc cgatgaccgc
     3301 ctggtaggga actcctcaag gaccgctgtc tatgacaatg gcaccctgga catcttcatc
     3361 accacatctc aggacagtgg tgccttcacc tgcattgctg ccaatgctgc cggagaggcc
     3421 acggccatgg tggaggtctc catcgtccag ctgccacacc tcagcaacag caccagccgc
     3481 actgcacccc ccaagtcccg cctctcagac atcactggct ccagcaagac cagccgggga
     3541 ggtggaggca gtgggggcgg agagcctccc aaaagccccc cggaacgggc tgtgcttgtg
     3601 tctgaagtga ccaccacctc ggccctggtc aagtggtctg tcagcaagtc agcaccccgg
     3661 gtgaagatgt accagctgca gtacaactgc tctgacgatg aggtactgat ttacaggatg
     3721 atcccagcct ccaacaaggc cttcgtggtc aacaacctgg tgtcagggac tggctacgac
     3781 ttgtgtgtgc tggccatgtg ggatgacaca gccacgacac tcacggccac caacatcgtg
     3841 ggctgcgccc agttcttcac caaggctgac tacccgcagt gccagtccat gcacagccag
     3901 attctgggcg gcaccatgat cctggtcatc gggggcatca tcgtggccac gctgctggtc
     3961 ttcatcgtca tcctcatggt gcgctacaag gtctgcaacc acgaggcccc cagcaagatg
     4021 gcagcggccg tgagcaatgt gtactcgcag accaacggcg cccagccacc gcctccaagc
     4081 agcgcaccag ccggggcccc gccgcagggc ccgccgaagg tggtggtgcg caacgagctc
     4141 ctggacttca ccgccagcct ggcccgcgcc agtgactcct cttcctccag ctccctgggc
     4201 agtggggagg ctgcggggct gggacgggcc ccctggagga tcccaccctc cgccccgcgc
     4261 cccaagccca gccttgaccg cctgatgggg gccttcgcct ccctggacct caagagtcag
     4321 agaaaggagg agctgctgga ctccaggact ccagccggga gaggggctgg gacgtcggcc
     4381 cggggccacc actcggaccg agagccactg ctggggcccc ctgcggcccg ggccaggagc
     4441 ctgctcccct tgccgttgga gggcaaggcc aaacgcagcc actccttcga catgggggac
     4501 tttgctgctg cggcggcggg aggggtcgtg ccgggcggct acagtcctcc tcggaaggtc
     4561 tcgaacatct ggacgaagcg cagcctctct gtcaacggca tgctcttgcc ctttgaggag
     4621 agtgacctgg tgggggcccg ggggactttt ggcagctccg aatgggtgat ggagagcacg
     4681 gtcggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     4741 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     4801 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     4861 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4921 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4981 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5041 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5101 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5161 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5221 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5281 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     5341 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     5401 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     5461 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     5521 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     5581 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     5641 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     5701 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     5761 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     5821 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     5881 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     5941 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     6001 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     6061 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6121 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6181 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6241 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6301 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6361 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6421 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6481 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6541 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6601 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6661 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6721 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6781 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6841 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6901 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6961 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7021 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7081 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7141 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7201 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7261 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7321 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7381 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     7441 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7501 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7561 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7621 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7681 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7741 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7801 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7861 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7921 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7981 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     8041 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8101 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9121 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9181 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9241 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9301 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9361 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9421 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9481 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9541 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9601 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9661 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9721 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9781 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9841 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9901 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9961 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10021 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10081 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10141 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10201 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10381 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10441 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10501 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10561 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10621 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
