LOCUS       V044179                 9844 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044179
VERSION     V044179
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..3904
                     /label="LPCAT1(NM_024830)"
                     /note="LPCAT1(NM_024830)"
                     /gene="LPCAT1"
     CDS             3911..3937
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3941..3967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3971..3997
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4042..4253
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4266..4534
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4569..5276
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5286..5339
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5340..5939
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5940..6527
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6601..6834
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6906..7027
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7046..7181
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7219..7235)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7243..7259
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7267..7297)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7312..7333
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7621..8209)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8380..9240)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9241..9345)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9819..9835
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaggct gcggggatgc ggaccccggg ccgcccctgc
     2341 ctccagcgca ggggccagcg acgctcggct gctggcgccc ccggggcgga accccttcgt
     2401 gcacgagctg cgcctcagcg ccctgcagaa ggcccaggtg gccctcatga cactgacgct
     2461 cttcccggtc cggctcctgg ttgccgctgc catgatgctg ctggcctggc ccctcgcact
     2521 tgtcgcatcc ctgggctctg cggagaagga acccgagcag cccccggccc tgtggaggaa
     2581 ggttgtggac ttcctgctga aggccatcat gcgcaccatg tggttcgccg gcggcttcca
     2641 ccgggtggcc gtgaaggggc ggcaggcgct gcccaccgag gcggccatcc tcacgctcgc
     2701 gcctcactcg tcctacttcg acgccatccc tgtgaccatg acgatgtcct ccatcgtgat
     2761 gaaggcagag agcagagaca tcccgatctg gggaactctg atccagtata tacggcctgt
     2821 gttcgtgtcc cggtcagacc aggattctcg caggaaaaca gtagaagaaa tcaagagacg
     2881 ggcgcagtcc aacggaaagt ggccacagat aatgattttt ccagaaggaa cttgtacaaa
     2941 caggacctgc ctaattacct tcaaacctgg tgcattcatc cctggagcgc ccgtccagcc
     3001 tgtggtttta cgatatccaa ataaactgga caccatcaca tggacgtggc aaggacctgg
     3061 agcgctggaa atcctgtggc tcacgctgtg tcagtttcac aaccaagtgg aaatcgagtt
     3121 ccttcctgtg tacagccctt ctgaggagga gaagaggaac cccgcgctgt atgccagcaa
     3181 cgtgcggcga gtcatggccg aggccttggg tgtctccgtg actgactaca cgttcgagga
     3241 ctgccagctg gccctggcgg aaggacagct ccgtctcccc gctgacactt gccttttaga
     3301 atttgccagg ctcgtgcggg gcctcgggct aaaaccagaa aagcttgaaa aagatctgga
     3361 cagatactca gaaagagcca ggatgaaggg aggagagaag ataggtattg cggagtttgc
     3421 cgcctccctg gaagtccccg tttctgactt gctggaagac atgttttcac tgttcgacga
     3481 gagcggcagc ggcgaggtgg acctgcgaga gtgtgtggtt gccctgtctg tcgtctgccg
     3541 gccggcccgg accctggaca ccatccagct ggctttcaag atgtacggag cgcaagagga
     3601 cggcagcgtc ggcgaaggtg acctgtcctg catcctcaag acggccctgg gggtggcaga
     3661 gctcaccgtg accgacctat tccgagccat tgaccaagag gagaagggga agatcacatt
     3721 cgctgacttc cacaggtttg cagaaatgta ccctgccttc gcagaggaat acctgtaccc
     3781 ggatcagaca catttcgaaa gctgtgcaga gacctcacct gcgccaatcc caaacggctt
     3841 ctgtgccgat ttcagcccgg aaaactcaga cgctgggcgg aagcctgttc gcaagaagct
     3901 ggatggatcc tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga
     3961 ttatgctagc tatccttatg atgtcccaga ctacgcatag gcggccgcga aggatctgcg
     4021 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4081 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa
     4141 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4201 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc
     4261 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     4321 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     4381 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     4441 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     4501 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag
     4561 acgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     4621 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     4681 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     4741 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     4801 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     4861 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     4921 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     4981 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     5041 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     5101 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     5161 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     5221 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat
     5281 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     5341 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     5401 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     5461 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5521 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5581 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     5641 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5701 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     5761 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5821 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5881 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     5941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6301 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc
     6481 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa
     6541 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6601 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     6661 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6721 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6781 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6841 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6901 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7021 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     7081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     7201 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7261 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7321 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7381 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7441 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7501 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7561 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7621 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7681 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7741 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7801 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7861 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7921 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7981 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8041 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8101 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8161 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8221 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8281 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8341 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8401 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8461 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8521 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8581 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8641 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8701 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8761 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8821 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8881 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8941 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9001 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9061 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9241 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9301 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9361 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9421 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9481 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9541 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     9601 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     9661 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     9721 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     9781 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     9841 gctg
//
