LOCUS       V026019                10105 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026019
VERSION     V026019
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4293
                     /label="LMNA(NM_001406991)"
                     /note="LMNA(NM_001406991)"
                     /gene="LMNA"
     CDS             4324..5034
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5075..5286
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5299..5567
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5594..6193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6200..6788
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6862..7095
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7167..7288
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7307..7442
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7480..7496)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7504..7520
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7528..7558)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7573..7594
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7882..8470)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8641..9501)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9502..9606)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10080..10096
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagacc ccgtcccagc ggcgcgccac ccgcagcggg
     2341 gcgcaggcca gctccactcc gctgtcgccc acccgcatca cccggctgca ggagaaggag
     2401 gacctgcagg agctcaatga tcgcttggcg gtctacatcg accgtgtgcg ctcgctggaa
     2461 acggagaacg cagggctgcg ccttcgcatc accgagtctg aagaggtggt cagccgcgag
     2521 gtgtccggca tcaaggccgc ctacgaggcc gagctcgggg atgcccgcaa gacccttgac
     2581 tcagtagcca aggagcgcgc ccgcctgcag ctggagctga gcaaagtgcg tgaggagttt
     2641 aaggagctga aagcgcgcaa taccaagaag gagggtgacc tgatagctgc tcaggctcgg
     2701 ctgaaggacc tggaggctct gctgaactcc aaggaggccg cactgagcac tgctctcagt
     2761 gagaagcgca cgctggaggg cgagctgcat gatctgcggg gccaggtggc caagcttgag
     2821 gcagccctag gtgaggccaa gaagcaactt caggatgaga tgctgcggcg ggtggatgct
     2881 gagaacaggc tgcagaccat gaaggaggaa ctggacttcc agaagaacat ctacagtgag
     2941 gagctgcgtg agaccaagcg ccgtcatgag acccgactgg tggagattga caatgggaag
     3001 cagcgtgagt ttgagagccg gctggcggat gcgctgcagg aactgcgggc ccagcatgag
     3061 gaccaggtgg agcagtataa gaaggagctg gagaagactt attctgccaa gctggacaat
     3121 gccaggcagt ctgctgagag gaacagcaac ctggtggggg ctgcccacga ggagctgcag
     3181 cagtcgcgca tccgcatcga cagcctctct gcccagctca gccagctcca gaagcagctg
     3241 gcagccaagg aggcgaagct tcgagacctg gaggactcac tggcccgtga gcgggacacc
     3301 agccggcggc tgctggcgga aaaggagcgg gagatggccg agatgcgggc aaggatgcag
     3361 cagcagctgg acgagtacca ggagcttctg gacatcaagc tggccctgga catggagatc
     3421 cacgcctacc gcaagctctt ggagggcgag gaggagaggc tacgcctgtc ccccagccct
     3481 acctcgcagc gcagccgtgg ccgtgcttcc tctcactcat cccagacaca gggtgggggc
     3541 agcgtcacca aaaagcgcaa actggagtcc actgagagcc gcagcagctt ctcacagcac
     3601 gcacgcacta gcgggcgcgt ggccgtggag gaggtggatg aggagggcaa gtttgtccgg
     3661 ctgcgcaaca agtccaatga ggaccagtcc atgggcaatt ggcagatcaa gcgccagaat
     3721 ggagatgatc ccttgctgac ttaccggttc ccaccaaagt tcaccctgaa ggctgggcag
     3781 gtggtgacga tctgggctgc aggagctggg gccacccaca gcccccctac cgacctggtg
     3841 tggaaggcac agaacacctg gggctgcggg aacagcctgc gtacggctct catcaactcc
     3901 actggggaag aagtggccat gcgcaagctg gtgcgctcag tgactgtggt tgaggacgac
     3961 gaggatgagg atggagatga cctgctccat caccaccacg gctcccactg cagcagctcg
     4021 ggggaccccg ctgagtacaa cctgcgctcg cgcaccgtgc tgtgcgggac ctgcgggcag
     4081 cctgccgaca aggcatctgc cagcggctca ggagcccagg tgggcggacc catctcctct
     4141 ggctcttctg cctccagtgt cacggtcact cgcagctacc gcagtgtggg gggcagtggg
     4201 ggtggcagct tcggggacaa tctggtcacc cgctcctacc tcctgggcaa ctccagcccc
     4261 cgaacccaga gcccccagaa ctgcagcatc atgggaggtg gaggttcagg tggaggtgga
     4321 tccatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     4381 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     4441 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4501 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     4561 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     4621 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     4681 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4741 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4801 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4861 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4921 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4981 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtagcggccg
     5041 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5101 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5161 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5221 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5281 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5341 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5401 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5461 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5521 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5581 gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5641 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5701 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6181 gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6241 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6301 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6361 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6421 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6481 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6541 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6601 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6661 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6721 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6781 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6841 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     6901 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6961 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7021 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7081 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7141 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7201 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7261 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7321 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7381 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7441 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     7501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8281 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9601 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9661 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     9781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     9841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9901 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     9961 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10021 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10081 taaaacgacg gccagtgcca agctg
//
