LOCUS       V037393                13759 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037393
VERSION     V037393
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7785
                     /label="LAMA4(NM_001105206)"
                     /note="LAMA4(NM_001105206)"
                     /gene="LAMA4"
     CDS             7792..7857
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7901..8112
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8125..8393
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8422..8431
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8452..9114
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9184..9237
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9238..9837
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9838..10426
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10500..10733
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10805..10926
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10966..11101
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11134..11150)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11158..11174
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11182..11212)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11227..11248
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11536..12124)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12295..13155)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13156..13260)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13734..13750
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctttgagctc agcctggcgc
     2341 tcggttctgc ctctgtggct cctctggagc gctgcctgct cccgcgccgc gtccggggac
     2401 gacaacgctt ttccttttga cattgaaggg agctcagcgg ttggcaggca agacccgcct
     2461 gagacgagcg aaccccgcgt ggctctggga cgcctgccgc ctgcggccga gaaatgcaat
     2521 gctggattct ttcacaccct gtcgggagaa tgtgtgccct gcgactgtaa tggcaattcc
     2581 aacgagtgtt tggacggctc aggatactgt gtgcactgcc agcggaacac aacaggagag
     2641 cactgtgaaa agtgtctgga tggttatatc ggagattcca tcaggggagc accccaattc
     2701 tgccagccgt gcccctgtcc cctgccccac ttggccaatt ttgcagaatc ctgctatagg
     2761 aaaaatggag ctgttcggtg catttgtaac gaaaattatg ctggacctaa ctgtgaaaga
     2821 tgtgctcccg gttactatgg aaacccctta ctcattggaa gcacctgtaa gaaatgtgac
     2881 tgcagtggaa attcagatcc caacctgatc tttgaagatt gtgatgaagt cactggccag
     2941 tgtaggaatt gcttacgcaa caccaccgga ttcaagtgtg aacgttgcgc tcctggctac
     3001 tatggggacg ccaggatagc caagaactgt gcagtgtgca actgcggggg aggcccatgt
     3061 gacagtgtaa ccggagaatg cttggaagaa ggttttgaac cccctacagg catggactgc
     3121 ccaaccataa gctgtgataa gtgcgtctgg gacctgactg atgacctgcg gttagcagcg
     3181 ctctccatcg aggaaggcaa atccggggtg ctgagcgtat cctctggggc cgccgctcat
     3241 aggcacgtga atgaaatcaa cgccaccatc tacctcctca aaacaaaatt gtcagaaaga
     3301 gaaaaccaat acgccctaag aaagatacaa atcaacaatg ctgagaacac gatgaaaagc
     3361 cttctgtctg acgtagagga attagttgaa aaggaaaatc aagcctccag aaaaggacaa
     3421 cttgttcaga aggaaagcat ggacaccatt aaccacgcaa gtcagctggt agagcaagcc
     3481 catgatatga gggataaaat ccaagagatc aacaacaaga tgctctatta tggggaagag
     3541 catgaactta gccccaagga aatctctgag aagctggtgt tggcccagaa gatgcttgaa
     3601 gagattagaa gccgtcaacc atttttcacc caacgggagc tcgtggatga ggaggcagat
     3661 gaggcttacg aactactgag ccaggctgag agctggcagc ggctgcacaa tgagacccgc
     3721 actctgtttc ctgtcgtcct ggagcagctg gatgactaca atgctaagtt gtcagatctc
     3781 caggaagcac ttgaccaggc ccttaactat gtcagggatg ccgaagacat gaacagggcc
     3841 acagcagcca ggcagcggga ccatgagaaa caacaggaaa gagtgaggga acaaatggaa
     3901 gtggtgaaca tgtctctgag cacatctgcg gactctctga caacacctcg tctaactctt
     3961 tcagaacttg atgatataat aaagaatgcg tcagggattt atgcagaaat agatggagcc
     4021 aaaagtgaac tacaagtaaa actatctaac ctaagtaacc tcagccatga tttagtccaa
     4081 gaagctattg accatgcaca ggaccttcaa caagaagcta atgaattgag caggaagttg
     4141 cacagttcag atatgaacgg gctggtacag aaggctttgg atgcatcaaa tgtctatgaa
     4201 aatattgtta attatgttag tgaagccaat gaaacagcag aatttgcttt gaacaccact
     4261 gaccgaattt atgatgcggt gagtgggatt gatactcaaa tcatttacca taaagatgaa
     4321 agtgagaacc tcctcaatca agccagagaa ctgcaagcaa aggcagagtc tagcagtgat
     4381 gaagcagtgg ctgacactag caggcgtgtg ggtggagccc tagcaaggaa aagtgccctt
     4441 aaaaccagac tcagtgatgc cgttaagcaa ctacaagcag cagagagagg ggatgcccag
     4501 cagcgcctgg ggcagtctag actgatcacc gaggaagcca acaggacgac gatggaggtg
     4561 cagcaggcca ctgcccccat ggccaacaat ctaaccaact ggtcacagaa tcttcaacat
     4621 tttgactctt ctgcttacaa cactgcagtg aactctgcta gggatgcagt aagaaatctg
     4681 accgaggttg tccctcagct cctggatcag cttcgtacgg ttgagcagaa gcgacctgca
     4741 agcaacgttt ctgccagcat ccagaggatc cgagagctca ttgctcagac cagaagtgtt
     4801 gccagcaaga tccaagtctc catgatgttt gatggccagt cagctgtgga agtgcactcg
     4861 agaaccagta tggatgactt aaaggccttc acgtctctga gcctgtacat gaaaccccct
     4921 gtgaagcggc cggaactgac cgagactgca gatcagttta tcctgtacct cggaagcaaa
     4981 aacgccaaaa aagagtatat gggtcttgca atcaaaaatg ataatctggt atacgtctat
     5041 aatttgggaa ctaaagatgt ggagattccc ctggactcca agcccgtcag ttcctggcct
     5101 gcttacttca gcattgtcaa gattgaaagg gtgggaaaac atggaaaggt gtttttaaca
     5161 gtcccgagtc taagtagcac agcagaggaa aagttcatta aaaaggggga attttcggga
     5221 gatgactctc tgctggacct ggaccctgag gacacagtgt tttatgttgg tggagtgcct
     5281 tccaacttca agctccctac cagcttaaac ctgcctggct ttgttggctg cctggaactg
     5341 gccactttga ataatgatgt gatcagcttg tacaacttta agcacatcta taatatggac
     5401 ccctccacat cagtgccatg tgcccgagat aagctggcct tcactcagag tcgggctgcc
     5461 agttacttct tcgatggctc cggttatgcc gtggtgagag acatcacaag gagagggaaa
     5521 tttggtcagg tgactcgctt tgacatagaa gttcgaacac cagctgacaa cggccttatt
     5581 ctcctgatgg tcaatggaag tatgtttttc agactggaaa tgcgcaatgg ttacctacat
     5641 gtgttctatg attttggatt cagcggtggc cctgtgcatc ttgaagatac gttaaagaaa
     5701 gctcaaatta atgatgcaaa ataccatgag atctcaatca tttaccacaa tgataagaaa
     5761 atgatcttgg tagttgacag aaggcatgtc aagagcatgg ataatgaaaa gatgaaaata
     5821 ccttttacag atatatacat tggaggagct cctccagaaa tcttacaatc cagggccctc
     5881 agagcacacc ttcccctaga tatcaacttc agaggatgca tgaagggctt ccagttccaa
     5941 aagaaggact tcaatttact ggagcagaca gaaaccctgg gagttggtta tggatgccca
     6001 gaagactcac ttatatctcg cagagcatat ttcaatggac agagcttcat tgcttcaatt
     6061 cagaaaatat ctttctttga tggctttgaa ggaggtttta atttccgaac attacaacca
     6121 aatgggttac tattctatta tgcttcaggg tcagacgtgt tctccatctc actggataat
     6181 ggtactgtca tcatggatgt aaagggaatc aaagttcagt cagtagataa gcagtacaat
     6241 gatgggctgt cccacttcgt cattagctct gtctcaccca caagatatga actgatagta
     6301 gataaaagca gagttgggag taagaatcct accaaaggga aaatagaaca gacacaagca
     6361 agtgaaaaga agttttactt cggtggctca ccaatcagtg ctcagtatgc taatttcact
     6421 ggctgcataa gtaatgccta ctttaccagg gtggatagag atgtggaggt tgaagatttc
     6481 caacggtata ctgaaaaggt ccacacttct ctttatgagt gtcccattga gtcttcacca
     6541 ttgtttctcc tccataaaaa aggaaaaaat ttatccaagc ctaaagcaag tcagaataaa
     6601 aagggaggga aaagtaaaga tgcaccttca tgggatcctg ttgctctgaa actcccagag
     6661 cggaatactc caagaaactc tcattgccac ctttccaaca gccctagagc aatagagcac
     6721 gcctatcaat atggaggaac agccaacagc cgccaagagt ttgaacactt aaaaggagat
     6781 tttggtgcca aatctcagtt ttccattcgt ctgagaactc gttcctccca tggcatgatc
     6841 ttctatgtct cagatcaaga agagaatgac ttcatgactc tatttttggc ccatggccgc
     6901 ttggtttaca tgtttaatgt tggtcacaaa aaactgaaga ttagaagcca ggagaaatac
     6961 aatgatggcc tgtggcatga tgtgatattt attcgagaaa ggagcagtgg ccgactggta
     7021 attgatggtc tccgagtcct agaagaaagt cttcctccta ctgaagctac ctggaaaatc
     7081 aagggtccca tttatttggg aggtgtggct cctggaaagg ctgtgaaaaa tgttcagatt
     7141 aactccatct acagttttag tggctgtctc agcaatctcc agctcaatgg ggcctccatc
     7201 acctctgctt ctcagacatt cagtgtgacc ccttgctttg aaggccccat ggaaacagga
     7261 acttactttt caacagaagg aggatacgtg gttctagatg aatctttcaa tattggattg
     7321 aagtttgaaa ttgcatttga agtccgtccc agaagcagtt ccggaaccct ggtccacggc
     7381 cacagtgtca atggggagta cctaaatgtt cacatgaaaa atggacaggt catagtgaaa
     7441 gtcaataatg gcatcagaga tttttccacc tcagttacac ccaagcagag tctctgtgat
     7501 ggcagatggc acagaattac agttattaga gattctaatg tggttcagtt ggatgtggac
     7561 tctgaagtga accatgtggt tggacccctg aatccaaaac caattgatca cagggagcct
     7621 gtgtttgttg gaggtgttcc agaatctcta ctgacaccac gcttggcccc cagcaaaccc
     7681 ttcacaggct gcatacgcca ctttgtgatt gatggacacc cagtgagctt cagtaaagca
     7741 gccctggtca gcggcgccgt aagcatcaac tcctgtccag cagccggatc cgactacaaa
     7801 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     7861 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     7921 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     7981 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     8041 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     8101 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     8161 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     8221 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     8281 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     8341 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     8401 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     8461 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     8521 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     8581 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     8641 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     8701 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     8761 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     8821 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     8881 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     8941 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     9001 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     9061 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     9121 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     9181 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     9241 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     9301 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     9361 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     9421 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     9481 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     9541 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     9601 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     9661 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     9721 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     9781 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     9841 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     9901 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     9961 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
    10021 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
    10081 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
    10141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
    10201 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
    10261 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
    10321 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
    10381 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
    10441 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    10501 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
    10561 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10621 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10681 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    10741 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    10801 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10921 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
    10981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    11041 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11101 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
    11161 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    11221 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    12001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12601 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12901 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    13021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    13321 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    13381 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    13441 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    13501 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    13561 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    13621 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    13681 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    13741 gacggccagt gccaagctg
//
