LOCUS       V029547                10003 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029547
VERSION     V029547
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4029
                     /label="Klhl28(NM_025707)"
                     /note="Klhl28(NM_025707)"
                     /gene="Klhl28"
     CDS             4036..4101
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4145..4356
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4369..4637
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4666..4675
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4696..5358
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5428..5481
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5482..6081
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6082..6670
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6744..6977
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7049..7170
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7210..7345
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7378..7394)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7402..7418
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7426..7456)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7471..7492
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7780..8368)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8539..9399)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9400..9504)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9978..9994
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg accacacagc cccgacctac
     2341 atgcttgcta acttaaccca cttacattct gaacaacttc tgcagggatt gaatcttctt
     2401 cgccagcatc acgaactctg tgacatcatt cttcgagtag gtgatgttaa aattcatgct
     2461 cataaagtgg tccttgccag catcagccca tacttcaaag ctatgttcac tggcaacctt
     2521 tctgagaaag agaacagtga ggttgagttt cagtgcatcg atgaagctgc gctgcaggcc
     2581 attgtggagt acgcctatac gggcacagtt ttcatttctc aggacacagt tgaatctctc
     2641 ctgccagcag caaacttact ccagataaaa ctcgttctta aagaatgctg tgcatttctt
     2701 gaaagccaac ttgaccctgg taattgtatt ggaatttctc gttttgcgga gacttacggt
     2761 tgtcatgacc tttatttggc ggctactaaa ttcatatgcc agaattttga atctgtttgc
     2821 cagacagaag agttttttga gcttacacat gctgatttgg acgaaattgt ctccaatgac
     2881 tgcttgaatg tggctactga agagacagtt ttttatgcat tagaatcttg gatcaagtac
     2941 gatgtacaag aacggcagaa gtacttagcc cagctgctga acagtgtacg gttaccattg
     3001 ctgagcgtta agttcctcac taggctctat gaagcgaatc atcttatccg tgatgatcgc
     3061 acctgtaaac atctcttgaa tgaagcccta aagtaccact tcatgcctga acatagactc
     3121 tctcatcaga cagtcttgat gacacgacct cgctgtgctc ccaaagtact gtgtgcagta
     3181 ggaggaaaat ctggactctt tgcctgtttg gatagtgtgg agatgtactt tccccagaat
     3241 gactcttgga ttggtctggc gcccttaaat attcctcgct atgaattcgg aatatgtgtt
     3301 ttagaccaaa aagtctttgt tataggtggt attgaaacta gcgtgcgtcc tggtatgact
     3361 gtcagaaaac atgaaaattc agtggaatgt tggaatcctg atacaaacac ctggacttct
     3421 ctcgagagaa tgaacgagag ccggagtact ctcggagtgg cagtgctcgc gggagaagtt
     3481 tttgccttag gtggatatga cgggcagtct tacttgcaat ctgtagaaaa atacatcccc
     3541 aaaataaggc agtggcaacc tgtggcccca atgacaacca caagaagttg ttttgctgca
     3601 gctgtcctgg atggaatgct atacgccatt ggaggctatg gccccgctca catgaacagt
     3661 gtggagcgtt acgatccgag taaggactcc tgggagatgg ttgcgcccat ggctgataag
     3721 aggattcact ttggcgtcgg agtcatgcta ggttttattt tcgtggtagg tgggcataac
     3781 ggtgtctcac atttgtcaag cattgaaaga tatgatcctc atcaaaatca gtggactgtg
     3841 tgtagaccaa tgaaagagcc caggacagga gttggtgctg cagtaatcga taactacctg
     3901 tatgtagtcg gtggtcactc agggtcttcc tatctgaaca cagtacagaa atacgaccct
     3961 atctcagata catggctgga ttcagcaggc atgatatact gtcgctgcaa ttttggatta
     4021 actgcacttg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4081 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4141 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4201 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4261 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4321 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4381 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     4441 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4501 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4561 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4621 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     4681 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     4741 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     4801 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     4861 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     4921 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     4981 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     5041 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     5101 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     5161 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     5221 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     5281 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     5341 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     5401 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     5461 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     5521 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5581 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5641 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5701 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5761 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5821 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5881 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5941 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6001 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6061 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     6121 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6181 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6241 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6301 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6361 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6421 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6481 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6541 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6601 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6661 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6721 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     6781 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6961 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7021 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7141 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7201 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7261 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7321 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     7381 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7441 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7501 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7561 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7621 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7681 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7741 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7801 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7861 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7921 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7981 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8041 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8101 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8161 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8221 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8281 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8341 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8401 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8461 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8521 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8581 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8641 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8701 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8761 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8821 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8881 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8941 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9001 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9061 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9121 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9181 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9241 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9301 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9361 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9421 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9481 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9541 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9601 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     9661 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9721 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9781 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     9841 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9901 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9961 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
