LOCUS       V026256                10297 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026256
VERSION     V026256
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4320
                     /label="Kcnq1(NM_008434)"
                     /note="Kcnq1(NM_008434)"
                     /gene="Kcnq1"
     CDS             4330..4395
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4439..4650
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4663..4931
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4960..4969
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4990..5652
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5722..5775
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5776..6375
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6376..6964
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7038..7271
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7343..7464
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7504..7639
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7672..7688)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7696..7712
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7720..7750)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7765..7786
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8074..8662)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8833..9693)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9694..9798)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10272..10288
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg acacggcctc gtccccgccc
     2341 agtgctgaaa ggaagcgcgc gggttggagc cgcctgctag gcgcccggcg gggcagcgcg
     2401 gtggtcaaga agtgtccctt ctcactggag ctggccgaag gtggccctga gggcagcacg
     2461 gtctatgcgc ccatcgcgcc aaccggagcc cccgggctcg cgccccccat gtcgacccca
     2521 gtgtcgcccg ccccggcccc tgcagacctc ggcccacgtc cgcgggtgag ccttgacccg
     2581 cgggtctcca tctacagtgc gcgccgcccg ctgctggcgc gcacccacat ccagggccga
     2641 gtctacaact tcctcgagcg ccccacgggt tggaagtgtt tcgtgtacca cttcaccgtc
     2701 ttcctcattg ttctggtctg cctcatcttc agtgtcctgt ccactattga gcagtatgcc
     2761 gctctggcca ccgggaccct cttctggatg gagattgtcc ttgtggtgtt ctttgggaca
     2821 gaatatgtgg tccgcctctg gtctgcaggc tgccgcagca agtacgtggg catctggggc
     2881 cggctacgtt ttgcccggaa gcccatttcc atcattgacc tcatcgtggt tgtagcctct
     2941 atggttgtcc tctgcgtggg ttccaaagga caagtgttcg ccacatcagc tatcaggggt
     3001 atccgcttcc ttcagatcct gcggatgctg catgtcgatc gccagggggg tacctggagg
     3061 ctcctgggct ctgtagtctt cattcaccgc caggagctga tcaccaccct gtacattggc
     3121 tttctgggcc ttatcttctc ctcctacttt gtctacttgg ctgagaaaga tgcggtgaac
     3181 gagtccggcc gcatcgagtt tggcagctac gcagatgctc tgtggtgggg ggtggtcaca
     3241 gtcactacca ttggctacgg ggataaggta cctcagacgt gggttgggaa gaccatcgcc
     3301 tcctgtttct ctgtcttcgc catatccttc tttgcactcc cagcggggat acttggctct
     3361 gggttcgcgc tgaaggtcca gcagaagcag aggcagaagc acttcaaccg gcagatccca
     3421 gctgcagcct cactcatcca gactgcatgg aggtgctatg ccgctgagaa ccctgactca
     3481 gccacttgga agatctatgt ccggaagcct gctcggagtc acacgcttct gtcccccagc
     3541 cccaaaccta aaaagtctgt catggtaaag aagaagaagt tcaagctgga taaggataat
     3601 gggatgagtc ctggagagaa gatgttcaat gttcctcaca tcacttatga tcccccagag
     3661 gataggaggc cagaccattt ctccattgat ggctatgaca gctcagtaag gaagagccct
     3721 acactgctgg aagtaagcac accccatttc ttgagaacaa acagctttgc agaggacctg
     3781 gacctggaag gggagacact gctgaccccc atcacccatg tgtcacagct gcgggatcac
     3841 catcgggcca ccatcaaggt catcaggcgc atgcagtact ttgtagccaa gaagaaattc
     3901 cagcaagcac ggaagcccta cgacgtgcga gatgtcatcg agcagtactc ccagggccac
     3961 ctgaacctta tggtgcgcat taaagaacta cagagaaggc tggatcagtc cattgggaag
     4021 ccatctttgt tcatccccat ctcagaaaag agcaaagacc gtggcagtaa caccatcggt
     4081 gcccgtctga acagggtgga agacaaggtg acacaactgg accagagact ggtgatcatc
     4141 acagacatgc tccaccagct gctgtccatg caacaaggtg gtccaacctg caacagcagg
     4201 tcacaagttg tagccagcaa tgaaggtggc tccatcaacc ctgagctctt cctacccagc
     4261 aacagcctgc ccacctacga acaactgact gtgccccaga caggccctga tgagggttcc
     4321 tgaggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     4381 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     4441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     4501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     4681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     4741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     4801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     4861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     4921 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     4981 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     5041 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     5101 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     5161 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     5221 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     5281 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     5341 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     5401 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     5461 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     5521 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     5581 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     5641 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     5701 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     5761 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     5821 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5881 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5941 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6001 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6061 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6121 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6181 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6241 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6301 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6361 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6421 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6481 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6541 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6601 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6661 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6721 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6781 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6841 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6901 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6961 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7021 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     7081 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7261 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7321 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7441 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7501 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7561 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7621 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     7681 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7741 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7801 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7861 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7921 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7981 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8041 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8101 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8161 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8221 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8281 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8341 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8401 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8461 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8521 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8581 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8761 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8821 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8881 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8941 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9001 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9061 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9121 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9181 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9241 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9301 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9361 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9421 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9481 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9541 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9601 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9661 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9721 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9781 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     9841 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     9901 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     9961 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10021 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10081 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10141 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10201 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10261 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
