LOCUS V030179 10444 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030179 VERSION V030179 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4470 /label="KLHL4(NM_019117)" /note="KLHL4(NM_019117)" /gene="KLHL4" CDS 4477..4542 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4586..4797 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4810..5078 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5107..5116 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5137..5799 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5869..5922 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5923..6522 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6523..7111 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7185..7418 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7490..7611 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7651..7786 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7819..7835) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7843..7859 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7867..7897) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7912..7933 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8221..8809) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8980..9840) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9841..9945) /gene="bla" /label="AmpR promoter" primer_bind 10419..10435 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cagtgtctgg caagaaagag 2341 tttgatgtga aacagatcct aaggctacgc tggaggtggt ttagtcatcc ttttcaaggt 2401 tccaccaaca ctggaagctg tcttcagcag gaaggatatg agcatagagg gaccccggtt 2461 cagggcaggt tgaagagcca ctctcgggac agaaacggac tgaagaaaag caacagtcct 2521 gtccaccaca atatactggc accagtgcca ggaccggccc ctgcccatca gagagccgtt 2581 cagaatttgc agcaacataa tctgatagta cattttcaag caaatgaaga tactcctaaa 2641 tcagttccag agaagaattt attcaaagaa gcttgtgaga aacgcgcaca agatttggag 2701 atgatggctg atgacaatat agaagattct acagcaagat tagatacaca acactctgaa 2761 gacatgaatg ccaccagatc tgaagagcag ttccatgtta taaaccacgc agagcaaact 2821 cttcgtaaaa tggagaacta cttgaaagag aaacaactat gtgatgtgct actgattgca 2881 ggacacctcc gcatcccagc ccataggttg gttctcagcg cagtgtctga ttattttgct 2941 gcaatgttta ctaatgatgt gcttgaagcc aaacaagaag aggtcaggat ggaaggagta 3001 gatccaaatg cactaaattc cttggtgcag tatgcttaca caggagtcct gcaattgaaa 3061 gaagatacca ttgaaagttt gctggctgca gcttgtcttc tgcagctgac tcaggtcatt 3121 gatgtttgct ccaattttct cataaagcag ctccatcctt caaactgctt agggattcga 3181 tcatttggag atgcccaagg ctgtacagaa cttctgaacg tggcacacaa atacactatg 3241 gaacacttca ttgaggtaat aaaaaaccaa gaattcctcc tgcttccagc taatgaaatt 3301 tcaaaacttc tgtgcagtga tgacattaat gtgcctgatg aagagaccat ttttcatgct 3361 ctaatgcagt gggtggggca tgatgtgcag aataggcaag gagaactggg gatgctgctt 3421 tcttacatca gactgccatt actcccacca cagttactgg cagatcttga aaccagttcc 3481 atgtttactg gtgatcttga gtgtcagaag ctcctgatgg aagctatgaa gtatcatctt 3541 ttgcctgaga gaagatccat gatgcaaagc cctcggacaa agcctagaaa atcaactgtg 3601 ggggcacttt atgctgtagg aggcatggat gctatgaaag gtactactac tattgaaaaa 3661 tatgacctca ggaccaacag ttggctacat attggcacca tgaatggccg taggcttcaa 3721 tttggagtcg cagttattga taataagctc tatgtcgtgg gaggaagaga cggtttaaaa 3781 actttgaata cagtggaatg ttttaatcca gttggcaaaa tctggactgt gatgcctccc 3841 atgtcaacac atcggcacgg cttaggtgta gccactcttg aaggaccaat gtatgctgta 3901 ggtggtcatg atggatggag ctatctaaat actgtagaaa gatgggaccc tgagggacga 3961 cagtggaatt acgtagccag tatgtcaact cctagaagca cagttggtgt tgttgcatta 4021 aacaacaaat tatatgctat tggtggacgt gatggaagtt cctgcctcaa atcaatggaa 4081 tactttgacc cacacactaa caagtggagt ttgtgtgctc caatgtccaa aagacgtgga 4141 ggtgtgggag ttgccacata caatggattc ttatatgttg taggggggca tgatgcccct 4201 gcttccaacc attgctccag gctttctgac tgtgtggaac ggtatgatcc aaaaggtgat 4261 tcatggtcaa ctgtggcacc tctgagtgtt cctcgagatg ctgttgctgt gtgccctctt 4321 ggagacaaac tctacgtggt tggaggatat gacggacata cttatttgaa cacagttgag 4381 tcatatgatg cacagagaaa tgaatggaaa gaggaagttc ctgttaacat tggaagagct 4441 ggtgcatgtg ttgtagtggt gaagctaccc ggatccgact acaaagacca tgacggtgat 4501 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc 4561 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4621 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4681 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4741 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4801 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4861 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 4921 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 4981 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 5041 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 5101 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 5161 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 5221 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 5281 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 5341 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 5401 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5461 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5521 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5581 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5641 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5701 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5761 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5821 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 5881 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 5941 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 6001 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 6061 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 6121 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6181 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6241 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6301 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6361 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6421 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6481 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 6541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 6601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 6661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 6721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 6841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 6901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg 6961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 7021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 7081 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7141 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7201 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7261 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7321 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7381 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7441 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7501 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7621 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 7681 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 7741 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 7801 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7861 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7921 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7981 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 8041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 8101 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 8161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 8401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 8461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 8521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 8581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 8641 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 8701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 9001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 9061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 9121 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 9181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 9241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 9541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 9601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9961 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 10021 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 10081 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 10141 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 10201 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10261 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 10321 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 10381 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 10441 gctg //