LOCUS       V030179                10444 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030179
VERSION     V030179
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4470
                     /label="KLHL4(NM_019117)"
                     /note="KLHL4(NM_019117)"
                     /gene="KLHL4"
     CDS             4477..4542
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4586..4797
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4810..5078
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5107..5116
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5137..5799
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5869..5922
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5923..6522
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6523..7111
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7185..7418
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7490..7611
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7651..7786
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7819..7835)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7843..7859
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7867..7897)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7912..7933
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8221..8809)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8980..9840)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9841..9945)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10419..10435
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cagtgtctgg caagaaagag
     2341 tttgatgtga aacagatcct aaggctacgc tggaggtggt ttagtcatcc ttttcaaggt
     2401 tccaccaaca ctggaagctg tcttcagcag gaaggatatg agcatagagg gaccccggtt
     2461 cagggcaggt tgaagagcca ctctcgggac agaaacggac tgaagaaaag caacagtcct
     2521 gtccaccaca atatactggc accagtgcca ggaccggccc ctgcccatca gagagccgtt
     2581 cagaatttgc agcaacataa tctgatagta cattttcaag caaatgaaga tactcctaaa
     2641 tcagttccag agaagaattt attcaaagaa gcttgtgaga aacgcgcaca agatttggag
     2701 atgatggctg atgacaatat agaagattct acagcaagat tagatacaca acactctgaa
     2761 gacatgaatg ccaccagatc tgaagagcag ttccatgtta taaaccacgc agagcaaact
     2821 cttcgtaaaa tggagaacta cttgaaagag aaacaactat gtgatgtgct actgattgca
     2881 ggacacctcc gcatcccagc ccataggttg gttctcagcg cagtgtctga ttattttgct
     2941 gcaatgttta ctaatgatgt gcttgaagcc aaacaagaag aggtcaggat ggaaggagta
     3001 gatccaaatg cactaaattc cttggtgcag tatgcttaca caggagtcct gcaattgaaa
     3061 gaagatacca ttgaaagttt gctggctgca gcttgtcttc tgcagctgac tcaggtcatt
     3121 gatgtttgct ccaattttct cataaagcag ctccatcctt caaactgctt agggattcga
     3181 tcatttggag atgcccaagg ctgtacagaa cttctgaacg tggcacacaa atacactatg
     3241 gaacacttca ttgaggtaat aaaaaaccaa gaattcctcc tgcttccagc taatgaaatt
     3301 tcaaaacttc tgtgcagtga tgacattaat gtgcctgatg aagagaccat ttttcatgct
     3361 ctaatgcagt gggtggggca tgatgtgcag aataggcaag gagaactggg gatgctgctt
     3421 tcttacatca gactgccatt actcccacca cagttactgg cagatcttga aaccagttcc
     3481 atgtttactg gtgatcttga gtgtcagaag ctcctgatgg aagctatgaa gtatcatctt
     3541 ttgcctgaga gaagatccat gatgcaaagc cctcggacaa agcctagaaa atcaactgtg
     3601 ggggcacttt atgctgtagg aggcatggat gctatgaaag gtactactac tattgaaaaa
     3661 tatgacctca ggaccaacag ttggctacat attggcacca tgaatggccg taggcttcaa
     3721 tttggagtcg cagttattga taataagctc tatgtcgtgg gaggaagaga cggtttaaaa
     3781 actttgaata cagtggaatg ttttaatcca gttggcaaaa tctggactgt gatgcctccc
     3841 atgtcaacac atcggcacgg cttaggtgta gccactcttg aaggaccaat gtatgctgta
     3901 ggtggtcatg atggatggag ctatctaaat actgtagaaa gatgggaccc tgagggacga
     3961 cagtggaatt acgtagccag tatgtcaact cctagaagca cagttggtgt tgttgcatta
     4021 aacaacaaat tatatgctat tggtggacgt gatggaagtt cctgcctcaa atcaatggaa
     4081 tactttgacc cacacactaa caagtggagt ttgtgtgctc caatgtccaa aagacgtgga
     4141 ggtgtgggag ttgccacata caatggattc ttatatgttg taggggggca tgatgcccct
     4201 gcttccaacc attgctccag gctttctgac tgtgtggaac ggtatgatcc aaaaggtgat
     4261 tcatggtcaa ctgtggcacc tctgagtgtt cctcgagatg ctgttgctgt gtgccctctt
     4321 ggagacaaac tctacgtggt tggaggatat gacggacata cttatttgaa cacagttgag
     4381 tcatatgatg cacagagaaa tgaatggaaa gaggaagttc ctgttaacat tggaagagct
     4441 ggtgcatgtg ttgtagtggt gaagctaccc ggatccgact acaaagacca tgacggtgat
     4501 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     4561 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4621 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4681 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4741 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4801 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4861 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4921 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     4981 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5041 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5101 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5161 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5221 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5281 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5341 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5401 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     5461 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5521 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     5581 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     5641 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     5701 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     5761 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     5821 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     5881 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     5941 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6001 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6061 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6121 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6181 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6241 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6301 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6361 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6421 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6481 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7081 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7141 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7201 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7261 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7321 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7381 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7441 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7501 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7621 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7681 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7741 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     7801 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7861 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7921 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7981 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8101 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8641 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9121 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9961 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10021 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10081 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10141 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10201 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10261 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10321 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10381 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10441 gctg
//
