LOCUS       V028502                11152 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028502
VERSION     V028502
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6006
                     /label="KLHDC7B(NM_138433)"
                     /note="KLHDC7B(NM_138433)"
                     /gene="KLHDC7B"
     CDS             6013..6078
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6122..6333
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6346..6614
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6641..7240
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7247..7835
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7909..8142
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8214..8335
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8354..8489
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8527..8543)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8551..8567
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8575..8605)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8620..8641
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8929..9517)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9688..10548)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10549..10653)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11127..11143
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatccag ggcaccttgg agccagatgg tcccctctgg
     2341 ggctgggact gggacagtga caatgactgg gatagtgctg tgctggccct cctggcgctg
     2401 gctgtggtgg ctgccacagc gctggcctta cactggtttg gctccgggca cgatcaagag
     2461 gcggcagaac cggtgtccac agccctcggg gctcaacctc atcaggcagg aggagctgag
     2521 ctggccctgc aaccgaagtc taaggtcagt gatggcagcg aggggcagag cccagggcag
     2581 gggaaaccag agcccccagg acgcggccag cagagccctg tccctgctgc agcgccgggc
     2641 gggggcctgg ccgccatggc ccggcttcca ctcaagacgg ctgtcgagga ggcccgcaga
     2701 gaggcattag gacagcaacg gggcagtgcc acccccgcgg ccccccgagc ggaaggaaag
     2761 gagcctccca ggccaggcac tgccctcctg ggcaggagcg aagcaggggg gatgtccgcc
     2821 cccctcctga tccacttcac tcctcggagc cctggcagcg aagcggaggc ggagacaggt
     2881 ggtgtcaggg cgtcctctcg ccaggccgca ggccccgcgg ggcaacagga cactggcccc
     2941 tggcaggcgg gcgcggggcc ctcgggctcg atggggagag gccggggccg gcggcggcgg
     3001 atggacgctg gctcgggaga cagagcccgc cgcccccgga aactggaccc gctccgcctg
     3061 ggcgccgcgg ggagcgtgtg ggacgcggtg gacggggccg ccgccctgga cgcccacgcg
     3121 cgcggcctcc ccacaggacc cccactcgcc caggagcccg cactcccggc gctgcccgct
     3181 ccccgcgccc tgcagcctgg gtctcagacg gaaggctctg gggccaaggg tggctggagc
     3241 agggaggcct cgggggtccc tgcccccgga ggaggctggc cctgggtcag cagggaggtc
     3301 ccgggcaccc ggagctttgg cccagcccca gactccacgc gcccctggct agagagtccg
     3361 cctcaaggtc gcccactctc gtcccaaggg ccgggtgcca caggggccta cgatgccggc
     3421 gaggccgggg ctgacagctc ccgagataac agtcctgccg ctgacctggg gcccacccgg
     3481 cccccggagc aagcaaagcc ggctgcagcc ggccacagcc gcgcgccctc ccggagccgt
     3541 gagcctcgcc cgcgctccgc ctccccgccc gcagctcccg gcccggggtt cccacctgaa
     3601 gccctgactc tcccctctcc ttcagacttt ttgcccctgg aggttaccca ggatccttcc
     3661 gtgggcgaaa atctcagagc ggcgccagcc ccaagttcag cctcagccca agtcttaact
     3721 tcagctccag cctcagtcct agccccagcc ctggcttcat cccccagctc agcaccaacc
     3781 tcagccacca cctcaacctc atcccccacc tcagccccag ccccagctcc aacctcagct
     3841 ccaacttcaa ccccagcccc agccccaagt ccagctgcag ccgcaactcc agccccagcc
     3901 ccagtcccag tcccaaccct cacaccccca tccccagccc taaccccagt cccaacccca
     3961 gccctaagcc cagctccaac tccagcccta accccagccg catccccagc cctaacccca
     4021 gtcccaaccc cagccctaag cccagctcca actccagccc caaccccagc cgcatcccct
     4081 gccccagccc ccacctcagc cccaacccca accccagccg catcccctgc cccagctgac
     4141 gggtcaaagc ctcaggagag tgtggctctc cccaggcgct accaggaggg gcaggtctca
     4201 gccagctggg gaaaccttat tgccatggtt cttagaagcc accccttccc caggcaagac
     4261 aggccccaag ggagtgtccc gagggcggtt cccgggagcc ccgtgggtcc cagcacttcc
     4321 acacactctg aggacagaca cggcccctct tcttcagtgg ggacagtcat agggacaggt
     4381 acagggggcc tggttgaggc tggaggtcag ccacagccaa gaagctccga gaccaacgga
     4441 tcgcccagcc cagaccctcc cccaggccta agaggagagg gaaccaggga gaaaagtcta
     4501 gacccgctgc cccaagccgc gatgcccagg ggccccgcac agccccccgc gcagaggccg
     4561 cctggccccg cggcctcctc ctctgcgagg cgctcacagc cggtacccca gctacggaaa
     4621 cgcagcaggt gcgaaatcgc cccgagctcg gagcaggagg tcaggccggc cgcctcgggg
     4681 gaccctcaag gggaggcgcc gggggagggg ggcagccctg ccggccgcag cggggcgctc
     4741 acggaaaagc aggaggaggc ccggaagctc atggtgtttc tgcagaggcc cgggggttgg
     4801 ggggtggtgg aggggccccg gaagcccagc tcccgggccc tggagcccgc cacggcggca
     4861 gccctgcggc ggcggctgga cctgggcagt tgcctggacg tgctggcctt tgcccagcag
     4921 cacggagagc ccggcctggc gcaggagacc tacgcgctga tgagcgacaa cctgctgcga
     4981 gtgctgggag acccgtgcct ctaccgccgg ctgagcgcgg ccgaccgcga gcgcatcctc
     5041 agcctgcgga ccggccgggg ccgggcggtg ctgggcgtcc tcgtactgcc cagcctctac
     5101 caggggggcc gctcagggct ccccaggggc cctcgtggcg aggagcctcc tgcggcggcc
     5161 cctgtgtccc tgcctctacc tgcgcacctg catgtgttca acccccggga gaacacctgg
     5221 cggcccctga cccaggtgcc cgaggaggcc ccgcttcggg gctgcggtct ctgcaccatg
     5281 cacaactacc tgtttctggc ggggggcatc cgtggctccg gtgccaaggc cgtctgctcc
     5341 aacgaggtct tctgctacaa ccctctgacc aacatctgga gccaggttcg gcccatgcag
     5401 caggcccgag cccagctcaa gctggtggcc ctggacgggc tgctctatgc catcggtggc
     5461 gaatgcctgt acagcatgga gtgctacgac ccgcgaacag acgcctggac cccacgcgcg
     5521 ccactccccg caggcacctt ccctgtggcc cacgaggctg tggcctgccg tggggacatc
     5581 tacgtcaccg ggggtcacct cttctaccgc ctgctcaggt acagccccgt gaaggatgct
     5641 tgggacgagt gcccatacag tgccagccac cggcgttcca gcgacatcgt ggcactgggg
     5701 ggcttcctgt accgcttcga cctgctgcgg ggcgtgggcg ccgccgtgat gcgctacaac
     5761 acagtgaccg gctcctggag cagggctgcc tccctgcccc tgcccgcccc cgccccactg
     5821 cactgcacca ccctgggcaa caccatttac tgcctcaacc cccaggtcac tgccaccttc
     5881 acggtctctg gggggactgc ccagttccag gccaaggagc tgcagccctt ccccttgggg
     5941 agcaccgggg tcctcagtcc attcatcctg actctgcccc ctgaggaccg gctgcagacc
     6001 tcactcggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6061 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6121 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6181 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6241 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6301 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6601 cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac
     6661 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     6721 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     6781 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6841 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6901 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6961 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7021 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7081 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7141 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7201 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga
     7261 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7321 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7381 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7441 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7501 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7561 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7621 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7681 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7741 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7801 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     7861 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7921 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7981 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8041 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8101 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8341 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8461 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     8521 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8581 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8641 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8701 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9361 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9601 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9661 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9721 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9781 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9841 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9901 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9961 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10021 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10081 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10141 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10201 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10261 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10321 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10381 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10441 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10501 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10561 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10621 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10681 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10741 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10801 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10861 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10921 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10981 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    11041 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    11101 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
