LOCUS       V034866                13076 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034866
VERSION     V034866
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2296..2736
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4&nbsp;(truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /transl_table=1
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
                     /label="GAL4 DNA binding domain"
     CDS             4030..4086
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4087..4803
                     /codon_start=1
                     /product="enhanced GFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             4834..7965
                     /label="KLB(NM_175737)"
                     /note="KLB(NM_175737)"
                     /gene="KLB"
     CDS             7972..7995
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        8046..8257
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8270..8538
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8565..9164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9171..9759
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9833..10066
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10138..10259
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10278..10413
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10451..10467)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10475..10491
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10499..10529)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10544..10565
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10853..11441)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11612..12472)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12473..12577)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13051..13067
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga
     2341 cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac
     2401 tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca
     2461 gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa
     2521 gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga
     2581 ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag
     2641 actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag
     2701 agtagtaaca aaggtcaaag acagttgact gtatcgatgg acccatctgt gacgctgtgg
     2761 cagtttctgc tgcagctgct gagagagcaa ggcaatggcc acatcatctc ctggacttca
     2821 cgggatggtg gtgaattcaa gctggtggat gcagaggagg tggcccggct gtgggggcta
     2881 cgcaagaaca agaccaacat gaattacgac aagctcagcc gggccttgcg gtactactat
     2941 gacaagaaca tcatccgcaa ggtgagcggc cagaagttcg tctacaagtt tgtgtcctac
     3001 cctgaggtcg cagggtgctc cactgaggac tgcccgcccc agccagaggt gtctgttacc
     3061 tccaccatgc caaatgtggc ccctgctgct atacatgccg ccccagggga cactgtctct
     3121 ggaaagccag gcacacccaa gggtgcagga atggcaggcc caggcggttt ggcacgcagc
     3181 agccggaacg agtacatgcg ctcgggcctc tattccacct tcaccatcca gtctctgcag
     3241 ccgcagccac cccctcatcc tcggcctgct gtggtgctcc ccagtgcagc tcctgcaggg
     3301 gcagcagcgc ccccctcggg gagcaggagc accagtccaa gccccttgga ggcctgtctg
     3361 gaggctgaag aggccggctt gcctctgcag gtcatcctga ccccgcccga ggccccaaac
     3421 ctgaaatcgg aagagcttaa tgtggagccg ggtttgggcc gggctttgcc cccagaagtg
     3481 aaagtagaag ggcccaagga agagttggaa gttgcggggg agagagggtt tgtgccagaa
     3541 accaccaagg ccgagccaga agtccctcca caggagggcg tgccagcccg gctgcccgcg
     3601 gttgttatgg acaccgcagg gcaggcgggc ggccatgcgg cttccagccc tgagatctcc
     3661 cagccgcaga agggccggaa gccccgggac ctagagcttc cactcagccc gagcctgcta
     3721 ggtgggccgg gacccgaacg gaccccagga tcgggaagtg gctccggcct ccaggctccg
     3781 gggccggcgc tgaccccatc cctgcttcct acgcatacat tgaccccggt gctgctgaca
     3841 cccagctcgc tgcctcctag cattcacttc tggagcaccc tgagtcccat tgcgccccgt
     3901 agcccggcca agctctcctt ccagtttcca tccagtggca gcgcccaggt gcacatccct
     3961 tctatcagcg tggatggcct ctcgaccccc gtggtgctct ccccagggcc ccagaagcca
     4021 ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct
     4081 ggaccgatgg tgtctaaggg cgaagagctg ttcaccgggg tggtgcccat cctggtcgag
     4141 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4201 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4261 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     4321 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4381 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4441 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4501 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4561 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4621 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4681 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4741 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4801 aagggtggag gcggatcagg aggcggtgga tccatgaagc caggctgtgc ggcaggatct
     4861 ccagggaatg aatggatttt cttcagcact gatgaaataa ccacacgcta taggaataca
     4921 atgtccaacg ggggattgca aagatctgtc atcctgtcag cacttattct gctacgagct
     4981 gttactggat tctctggaga tggaagagct atatggtcta aaaatcctaa ttttactccg
     5041 gtaaatgaaa gtcagctgtt tctctatgac actttcccta aaaacttttt ctggggtatt
     5101 gggactggag cattgcaagt ggaagggagt tggaagaagg atggaaaagg accttctata
     5161 tgggatcatt tcatccacac acaccttaaa aatgtcagca gcacgaatgg ttccagtgac
     5221 agttatattt ttctggaaaa agacttatca gccctggatt ttataggagt ttctttttat
     5281 caattttcaa tttcctggcc aaggcttttc cccgatggaa tagtaacagt tgccaacgca
     5341 aaaggtctgc agtactacag tactcttctg gacgctctag tgcttagaaa cattgaacct
     5401 atagttactt tataccactg ggatttgcct ttggcactac aagaaaaata tggggggtgg
     5461 aaaaatgata ccataataga tatcttcaat gactatgcca catactgttt ccagatgttt
     5521 ggggaccgtg tcaaatattg gattacaatt cacaacccat atctagtggc ttggcatggg
     5581 tatgggacag gtatgcatgc ccctggagag aagggaaatt tagcagctgt ctacactgtg
     5641 ggacacaact tgatcaaggc tcactcgaaa gtttggcata actacaacac acatttccgc
     5701 ccacatcaga agggttggtt atcgatcacg ttgggatctc attggatcga gccaaaccgg
     5761 tcggaaaaca cgatggatat attcaaatgt caacaatcca tggtttctgt gcttggatgg
     5821 tttgccaacc ctatccatgg ggatggcgac tatccagagg ggatgagaaa gaagttgttc
     5881 tccgttctac ccattttctc tgaagcagag aagcatgaga tgagaggcac agctgatttc
     5941 tttgcctttt cttttggacc caacaacttc aagcccctaa acaccatggc taaaatggga
     6001 caaaatgttt cacttaattt aagagaagcg ctgaactgga ttaaactgga atacaacaac
     6061 cctcgaatct tgattgctga gaatggctgg ttcacagaca gtcgtgtgaa aacagaagac
     6121 accacggcca tctacatgat gaagaatttc ctcagccagg tgcttcaagc aataaggtta
     6181 gatgaaatac gagtgtttgg ttatactgcc tggtctctcc tggatggctt tgaatggcag
     6241 gatgcttaca ccatccgccg aggattattt tatgtggatt ttaacagtaa acagaaagag
     6301 cggaaaccta agtcttcagc acactactac aaacagatca tacgagaaaa tggtttttct
     6361 ttaaaagagt ccacgccaga tgtgcagggc cagtttccct gtgacttctc ctggggtgtc
     6421 actgaatctg ttcttaagcc cgagtctgtg gcttcgtccc cacagttcag cgatcctcat
     6481 ctgtacgtgt ggaacgccac tggcaacaga ctgttgcacc gagtggaagg ggtgaggctg
     6541 aaaacacgac ccgctcaatg cacagatttt gtaaacatca aaaaacaact tgagatgttg
     6601 gcaagaatga aagtcaccca ctaccggttt gctctggatt gggcctcggt ccttcccact
     6661 ggcaacctgt ccgcggtgaa ccgacaggcc ctgaggtact acaggtgcgt ggtcagtgag
     6721 gggctgaagc ttggcatctc cgcgatggtc accctgtatt atccgaccca cgcccaccta
     6781 ggcctccccg agcctctgtt gcatgccgac gggtggctga acccatcgac ggccgaggcc
     6841 ttccaggcct acgctgggct gtgcttccag gagctggggg acctggtgaa gctctggatc
     6901 accatcaacg agcctaaccg gctaagtgac atctacaacc gctctggcaa cgacacctac
     6961 ggggcggcgc acaacctgct ggtggcccac gccctggcct ggcgcctcta cgaccggcag
     7021 ttcaggccct cacagcgcgg ggccgtgtcg ctgtcgctgc acgcggactg ggcggaaccc
     7081 gccaacccct atgctgactc gcactggagg gcggccgagc gcttcctgca gttcgagatc
     7141 gcctggttcg ccgagccgct cttcaagacc ggggactacc ccgcggccat gagggaatac
     7201 attgcctcca agcaccgacg ggggctttcc agctcggccc tgccgcgcct caccgaggcc
     7261 gaaaggaggc tgctcaaggg cacggtcgac ttctgcgcgc tcaaccactt caccactagg
     7321 ttcgtgatgc acgagcagct ggccggcagc cgctacgact cggacaggga catccagttt
     7381 ctgcaggaca tcacccgcct gagctccccc acgcgcctgg ctgtgattcc ctggggggtg
     7441 cgcaagctgc tgcggtgggt ccggaggaac tacggcgaca tggacattta catcaccgcc
     7501 agtggcatcg acgaccaggc tctggaggat gaccggctcc ggaagtacta cctagggaag
     7561 taccttcagg aggtgctgaa agcatacctg attgataaag tcagaatcaa aggctattat
     7621 gcattcaaac tggctgaaga gaaatctaaa cccagatttg gattcttcac atctgatttt
     7681 aaagctaaat cctcaataca attttacaac aaagtgatca gcagcagggg cttccctttt
     7741 gagaacagta gttctagatg cagtcagacc caagaaaata cagagtgcac tgtctgctta
     7801 ttccttgtgc agaagaaacc actgatattc ctgggttgtt gcttcttctc caccctggtt
     7861 ctactcttat caattgccat ttttcaaagg cagaagagaa gaaagttttg gaaagcaaaa
     7921 aacttacaac acataccatt aaagaaaggc aagagagttg ttagcctcga ggattacaag
     7981 gacgacgatg acaagtaggg atccgcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     8041 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     8101 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     8161 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     8221 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     8281 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     8341 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     8401 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     8461 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     8521 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc
     8581 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     8641 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     8701 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8761 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8821 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8881 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8941 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     9001 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     9061 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9121 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc
     9181 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     9241 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     9301 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     9361 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     9421 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     9481 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     9541 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     9601 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     9661 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     9721 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     9781 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     9841 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta
     9901 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9961 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10021 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
    10081 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
    10141 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10201 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10261 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10321 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    10381 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
    10441 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10501 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10561 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11281 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    12181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    12241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    12301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    12361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    12421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    12481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12541 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12601 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    12661 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    12721 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    12781 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    12841 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    12901 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    12961 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    13021 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
