LOCUS       V043564                11165 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043564
VERSION     V043564
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..6090
                     /label="KL(NM_004795)"
                     /note="KL(NM_004795)"
                     /gene="KL"
     promoter        6135..6346
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6359..6627
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6654..7253
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7260..7848
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7922..8155
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8227..8348
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8367..8502
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8540..8556)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8564..8580
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8588..8618)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8633..8654
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8942..9530)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9701..10561)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10562..10666)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11140..11156
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatgccc
     3061 gccagcgccc cgccgcgccg cccgcggccg ccgccgccgt cgctgtcgct gctgctggtg
     3121 ctgctgggcc tgggcggccg ccgcctgcgt gcggagccgg gcgacggcgc gcagacctgg
     3181 gcccgtttct cgcggcctcc tgcccccgag gccgcgggcc tcttccaggg caccttcccc
     3241 gacggcttcc tctgggccgt gggcagcgcc gcctaccaga ccgagggcgg ctggcagcag
     3301 cacggcaagg gtgcgtccat ctgggatacg ttcacccacc accccctggc acccccggga
     3361 gactcccgga acgccagtct gccgttgggc gccccgtcgc cgctgcagcc cgccaccggg
     3421 gacgtagcca gcgacagcta caacaacgtc ttccgcgaca cggaggcgct gcgcgagctc
     3481 ggggtcactc actaccgctt ctccatctcg tgggcgcgag tgctccccaa tggcagcgcg
     3541 ggcgtcccca accgcgaggg gctgcgctac taccggcgcc tgctggagcg gctgcgggag
     3601 ctgggcgtgc agcccgtggt caccctgtac cactgggacc tgccccagcg cctgcaggac
     3661 gcctacggcg gctgggccaa ccgcgccctg gccgaccact tcagggatta cgcggagctc
     3721 tgcttccgcc acttcggcgg tcaggtcaag tactggatca ccatcgacaa cccctacgtg
     3781 gtggcctggc acggctacgc caccgggcgc ctggcccccg gcatccgggg cagcccgcgg
     3841 ctcgggtacc tggtggcgca caacctcctc ctggctcatg ccaaagtctg gcatctctac
     3901 aatacttctt tccgtcccac tcagggaggt caggtgtcca ttgccctaag ctctcactgg
     3961 atcaatcctc gaagaatgac cgaccacagc atcaaagaat gtcaaaaatc tctggacttt
     4021 gtactaggtt ggtttgccaa acccgtattt attgatggtg actatcccga gagcatgaag
     4081 aataaccttt catctattct gcctgatttt actgaatctg agaaaaagtt catcaaagga
     4141 actgctgact tttttgctct ttgctttgga cccaccttga gttttcaact tttggaccct
     4201 cacatgaagt tccgccaatt ggaatctccc aacctgaggc aactgctttc ctggattgac
     4261 cttgaattta accatcctca aatatttatt gtggaaaatg gctggtttgt ctcagggacc
     4321 accaagagag atgatgccaa atatatgtat tacctcaaaa agttcatcat ggaaacctta
     4381 aaagccatca agctggatgg ggtggatgtc atcgggtata ccgcatggtc cctcatggat
     4441 ggtttcgagt ggcacagagg ttacagcatc aggcgtggac tcttctatgt tgactttcta
     4501 agccaggaca agatgttgtt gccaaagtct tcagccttgt tctaccaaaa gctgatagag
     4561 aaaaatggct tccctccttt acctgaaaat cagcccctag aagggacatt tccctgtgac
     4621 tttgcttggg gagttgttga caactacatt caagtagata ccactctgtc tcagtttacc
     4681 gacctgaatg tttacctgtg ggatgtccac cacagtaaaa ggcttattaa agtggatggg
     4741 gttgtgacca agaagaggaa atcctactgt gttgactttg ctgccatcca gccccagatc
     4801 gctttactcc aggaaatgca cgttacacat tttcgcttct ccctggactg ggccctgatt
     4861 ctccctctgg gtaaccagtc ccaggtgaac cacaccatcc tgcagtacta tcgctgcatg
     4921 gccagcgagc ttgtccgtgt caacatcacc ccagtggtgg ccctgtggca gcctatggcc
     4981 ccgaaccaag gactgccgcg cctcctggcc aggcagggcg cctgggagaa cccctacact
     5041 gccctggcct ttgcagagta tgcccgactg tgctttcaag agctcggcca tcacgtcaag
     5101 ctttggataa cgatgaatga gccgtataca aggaatatga catacagtgc tggccacaac
     5161 cttctgaagg cccatgccct ggcttggcat gtgtacaatg aaaagtttag gcatgctcag
     5221 aatgggaaaa tatccatagc cttgcaggct gattggatag aacctgcctg ccctttctcc
     5281 caaaaggaca aagaggtggc tgagagagtt ttggaatttg acattggctg gctggctgag
     5341 cccattttcg gctctggaga ttatccatgg gtgatgaggg actggctgaa ccaaagaaac
     5401 aattttcttc ttccttattt cactgaagat gaaaaaaagc taatccaggg tacctttgac
     5461 tttttggctt taagccatta taccaccatc cttgtagact cagaaaaaga agatccaata
     5521 aaatacaatg attacctaga agtgcaagaa atgaccgaca tcacgtggct caactccccc
     5581 agtcaggtgg cggtagtgcc ctgggggttg cgcaaagtgc tgaactggct gaagttcaag
     5641 tacggagacc tccccatgta cataatatcc aatggaatcg atgacgggct gcatgctgag
     5701 gacgaccagc tgagggtgta ttatatgcag aattacataa acgaagctct caaagcccac
     5761 atactggatg gtatcaatct ttgcggatac tttgcttatt cgtttaacga ccgcacagct
     5821 ccgaggtttg gcctctatcg ttatgctgca gatcagtttg agcccaaggc atccatgaaa
     5881 cattacagga aaattattga cagcaatggt ttcccgggcc cagaaactct ggaaagattt
     5941 tgtccagaag aattcaccgt gtgtactgag tgcagttttt ttcacacccg aaagtcttta
     6001 ctggctttca tagcttttct attttttgct tctattattt ctctctccct tatattttac
     6061 tactcgaaga aaggcagaag aagttacaaa taggcggccg cgaaggatct gcgatcgctc
     6121 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6181 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     6241 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6301 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     6361 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     6421 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     6481 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     6541 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     6601 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg
     6661 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     6721 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     6781 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6841 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6901 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6961 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7021 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7081 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7141 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7201 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca
     7261 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7321 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     7381 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7441 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7501 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7561 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7621 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7681 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7741 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7801 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     7861 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7921 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7981 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8041 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8101 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8161 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8221 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8281 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8341 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     8401 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8461 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     8521 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8581 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8641 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8701 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8761 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8821 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8881 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8941 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9001 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9061 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9121 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9181 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9241 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9301 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9361 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9421 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9481 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9541 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9601 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9661 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9721 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9781 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9841 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9901 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9961 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10021 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10081 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10141 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10201 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10261 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10321 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10381 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10441 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10501 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10561 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10621 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10681 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10741 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10801 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10861 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10921 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10981 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    11041 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11101 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    11161 agctg
//
