LOCUS       V033812                10960 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033812
VERSION     V033812
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4986
                     /label="KIF20A(NM_005733)"
                     /note="KIF20A(NM_005733)"
                     /gene="KIF20A"
     CDS             4993..5058
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5102..5313
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5326..5594
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5623..5632
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5653..6315
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6385..6438
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6439..7038
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7039..7627
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7701..7934
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8006..8127
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8167..8302
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8335..8351)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8359..8375
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8383..8413)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8428..8449
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8737..9325)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9496..10356)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10357..10461)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10935..10951
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgcaagggat cctttctccg
     2341 ccagcgggct tgctgtccga tgacgatgtc gtagtttctc ccatgtttga gtccacagct
     2401 gcagatttgg ggtctgtggt acgcaagaac ctgctatcag actgctctgt cgtctctacc
     2461 tccctagagg acaagcagca ggttccatct gaggacagta tggagaaggt gaaagtatac
     2521 ttgagggtta ggcccttgtt accttcagag ttggaacgac aggaagatca gggttgtgtc
     2581 cgtattgaga atgtggagac ccttgttcta caagcaccca aggactcttt tgccctgaag
     2641 agcaatgaac ggggaattgg ccaagccaca cacaggttca ccttttccca gatctttggg
     2701 ccagaagtgg gacaggcatc cttcttcaac ctaactgtga aggagatggt aaaggatgta
     2761 ctcaaagggc agaactggct catctataca tatggagtca ctaactcagg gaaaacccac
     2821 acgattcaag gtaccatcaa ggatggaggg attctccccc ggtccctggc gctgatcttc
     2881 aatagcctcc aaggccaact tcatccaaca cctgatctga agcccttgct ctccaatgag
     2941 gtaatctggc tagacagcaa gcagatccga caggaggaaa tgaagaagct gtccctgcta
     3001 aatggaggcc tccaagagga ggagctgtcc acttccttga agaggagtgt ctacatcgaa
     3061 agtcggatag gtaccagcac cagcttcgac agtggcattg ctgggctctc ttctatcagt
     3121 cagtgtacca gcagtagcca gctggatgaa acaagtcatc gatgggcaca gccagacact
     3181 gccccactac ctgtcccggc aaacattcgc ttctccatct ggatctcatt ctttgagatc
     3241 tacaacgaac tgctttatga cctattagaa ccgcctagcc aacagcgcaa gaggcagact
     3301 ttgcggctat gcgaggatca aaatggcaat ccctatgtga aagatctcaa ctggattcat
     3361 gtgcaagatg ctgaggaggc ctggaagctc ctaaaagtgg gtcgtaagaa ccagagcttt
     3421 gccagcaccc acctcaacca gaactccagc cgcagtcaca gcatcttctc aatcaggatc
     3481 ctacaccttc agggggaagg agatatagtc cccaagatca gcgagctgtc actctgtgat
     3541 ctggctggct cagagcgctg caaagatcag aagagtggtg aacggttgaa ggaagcagga
     3601 aacattaaca cctctctaca caccctgggc cgctgtattg ctgcccttcg tcaaaaccag
     3661 cagaaccggt caaagcagaa cctggttccc ttccgtgaca gcaagttgac tcgagtgttc
     3721 caaggtttct tcacaggccg aggccgttcc tgcatgattg tcaatgtgaa tccctgtgca
     3781 tctacctatg atgaaactct tcatgtggcc aagttctcag ccattgctag ccagcttgtg
     3841 catgccccac ctatgcaact gggattccca tccctgcact cgttcatcaa ggaacatagt
     3901 cttcaggtat cccccagctt agagaaaggg gctaaggcag acacaggcct tgatgatgat
     3961 attgaaaatg aagctgacat ctccatgtat ggcaaagagg agctcctaca agttgtggaa
     4021 gccatgaaga cactgctttt gaaggaacga caggaaaagc tacagctgga gatgcatctc
     4081 cgagatgaaa tttgcaatga gatggtagaa cagatgcaac agcgggaaca gtggtgcagt
     4141 gaacatttgg acacccaaaa ggaactattg gaggaaatgt atgaagaaaa actaaatatc
     4201 ctcaaggagt cactgacaag tttttaccaa gaagagattc aggagcggga tgaaaagatt
     4261 gaagagctag aagctctctt gcaggaagcc agacaacagt cagtggccca tcagcaatca
     4321 gggtctgaat tggccctacg gcggtcacaa aggttggcag cttctgcctc cacccagcag
     4381 cttcaggagg ttaaagctaa attacagcag tgcaaagcag agctaaactc taccactgaa
     4441 gagttgcata agtatcagaa aatgttagaa ccaccaccct cagccaagcc cttcaccatt
     4501 gatgtggaca agaagttaga agagggccag aagaatataa ggctgttgcg gacagagctt
     4561 cagaaacttg gtgagtctct ccaatcagca gagagagctt gttgccacag cactggggca
     4621 ggaaaacttc gtcaagcctt gaccacttgt gatgacatct taatcaaaca ggaccagact
     4681 ctggctgaac tgcagaacaa catggtgcta gtgaaactgg accttcggaa gaaggcagca
     4741 tgtattgctg agcagtatca tactgtgttg aaactccaag gccaggtttc tgccaaaaag
     4801 cgccttggta ccaaccagga aaatcagcaa ccaaaccaac aaccaccagg gaagaaacca
     4861 ttccttcgaa atttacttcc ccgaacacca acctgccaaa gctcaacaga ctgcagccct
     4921 tatgcccgga tcctacgctc acggcgttcc cctttactca aatctgggcc ttttggcaaa
     4981 aagtacggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     5041 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5101 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5161 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5221 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5281 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5341 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5401 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5461 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5521 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5581 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5641 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5701 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5761 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5821 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5881 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5941 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6001 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6061 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6121 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6181 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6241 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6301 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6361 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6421 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6481 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6541 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6601 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6661 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6721 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6781 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6841 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6901 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6961 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7021 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7081 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7141 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7201 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7261 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7321 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7381 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7441 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7501 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7561 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7621 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7681 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     7741 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7801 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7861 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7921 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7981 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8041 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8101 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8161 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8221 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8281 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10501 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10561 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10621 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10681 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10741 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10801 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10861 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10921 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
