LOCUS       V039934                13768 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039934
VERSION     V039934
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7794
                     /label="KIF13B(NM_015254)"
                     /note="KIF13B(NM_015254)"
                     /gene="KIF13B"
     CDS             7801..7866
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7910..8121
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8134..8402
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8431..8440
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8461..9123
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9193..9246
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9247..9846
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9847..10435
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10509..10742
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10814..10935
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10975..11110
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11143..11159)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11167..11183
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11191..11221)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11236..11257
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11545..12133)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12304..13164)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13165..13269)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13743..13759
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gggactccaa agtgaaagtg
     2341 gcggtgcgga tacgacccat gaaccggcga gagactgact tgcataccaa atgtgtggtg
     2401 gatgtggatg caaacaaggt tattcttaat cctgtaaata cgaatctttc caaaggagat
     2461 gcccggggcc agccgaaggt gtttgcttat gatcattgtt tctggtctat ggatgaatct
     2521 gtcaaagaaa agtatgcagg tcaagatatt gttttcaagt gccttggaga gaatatcctg
     2581 cagaatgctt ttgatggcta caatgcatgt atctttgcct atggacagac tggctctgga
     2641 aaatcttata ccatgatggg cacagctgac caacctggat taatcccaag actttgcagt
     2701 ggactctttg aacgaactca gaaagaggaa aatgaagaac agagttttaa agtagaagtg
     2761 tcctacatgg aaatttataa tgaaaaagtt cgagaccttc ttgatcccaa aggaagccgt
     2821 cagacgttga aagtcagaga gcatagtgtg ttgggacctt atgtcgacgg actttctaaa
     2881 ctggctgtca caagctacaa ggatattgag tcgttgatgt ctgagggtaa caaatctcgc
     2941 acagttgctg caaccaacat gaacgaggag agtagccgat cccatgcagt tttcaaaatc
     3001 accctcacac atactctcta cgatgtgaag tctgggacat ctggagagaa agtgggcaaa
     3061 ctcagcctgg tggatttagc tggcagtgaa cgagcaacga agacaggcgc tgcaggggac
     3121 aggctgaagg aagggagcaa cattaacaag tccctcacaa ccctcggtct ggttatctca
     3181 gctcttgcag atcagagtgc tggcaaaaac aagaataaat ttgttccata tcgtgactca
     3241 gttctcactt ggctgctcaa agacagcctc gggggtaaca gcaagaccgc catggtggct
     3301 actgtgagtc ctgcagctga taactatgat gaaaccctct caactctgcg gtatgcagat
     3361 cgagccaagc acattgtaaa ccacgctgtg gtgaatgagg accctaatgc ccgaattatc
     3421 cgggatctcc gggaagaagt tgagaaactc cgggagcagc tgaccaaagc agaggcaatg
     3481 aaatctccag agctaaagga ccggctggaa gaatctgaga agctaatcca ggaaatgact
     3541 gtgacctggg aggagaaatt aaggaaaacg gaggagattg cacaggaacg acagaaacag
     3601 cttgagagtc ttggaatatc tcttcagtct tcgggaatca aagttgggga tgataaatgc
     3661 ttccttgtga atctgaatgc tgacccagct ctgaatgagc ttctggtgta ctatttaaag
     3721 gaacatacat tgatagggtc agcaaattcc caagatatcc aactgtgcgg catgggaatt
     3781 cttcctgaac actgtattat agacatcacg tcagaaggcc aggttatgct gactcctcag
     3841 aagaacacca gaacatttgt aaatgggtca tctgtctcca gtccaataca gctacaccat
     3901 ggggacagga tattatgggg aaacaatcat ttcttcagac tcaatttgcc taaaaagaaa
     3961 aagaaagcag aacgagagga tgaggaccag gatccctcca tgaagaacga gaatagttct
     4021 gagcagctgg atgtagacgg agactcctcc agcgaggtgt ccagtgaagt taactttaat
     4081 tacgaatacg cacagatgga ggtcaccatg aaggccctgg gcagcaatga tccgatgcag
     4141 tccatattaa acagcttaga acaacagcat gaagaagaaa aacgatctgc actggagcgc
     4201 cagaggctta tgtatgagca cgaattggag cagctccgga gaaggctgtc tcctgagaag
     4261 cagaactgcc ggagcatgga caggttttct ttccactcgc ccagcgctca gcaacgctta
     4321 agacagtggg ctgaggagag agaagcaacg ttgaataaca gcctgatgag gctgagggaa
     4381 caaattgtta aggccaatct attggtgaga gaagctaatt acattgctga ggagctggat
     4441 aaaagaacag aatacaaagt taccctacag attccagcct ccagcctgga tgccaacagg
     4501 aagcgaggct ctcttcttag tgagcctgca atccaggtga gaagaaaagg aaaaggaaag
     4561 cagatttggt ctttggaaaa actggacaac aggctgttgg atatgagaga cctttatcag
     4621 gagtggaaag agtgtgaaga agataaccca gtaatacgat catacttcaa acgtgctgat
     4681 ccattctatg atgagcagga aaatcacagt ctcattgggg tggccaatgt cttcctcgag
     4741 tcacttttct atgatgtgaa gttacaatac gctgttccca tcatcaacca gaaaggagag
     4801 gtggcaggtc ggctgcacgt ggaggtgatg cgactcagtg gtgatgttgg ggagaggatc
     4861 gcaggaggcg atgaggtggc agaggtctcc tttgagaagg agacccagga gaacaaactg
     4921 gtgtgcatgg ttaaaatcct gcaagctact gggttgccac agcatctgtc ccactttgtg
     4981 ttctgcaaat acagcttctg ggatcaacag gagccggtga ttgtcgctcc tgaagtggac
     5041 acctcctcct cttccgtcag caaggagccg cactgcatgg ttgtctttga tcattgcaat
     5101 gagttttctg ttaacatcac cgaagacttt atcgagcatc tttccgaagg agcattggca
     5161 attgaagtat atggacataa aataaacgat ccccggaaaa accccgccct gtgggatttg
     5221 ggaatcatcc aagcaaagac acgtagtctt cgggacagat ggagtgaagt gaccaggaaa
     5281 ttggaattct gggttcaaat cttggaacag aatgagaatg gtgaatactg ccctgtagaa
     5341 gtgatttctg caaaggatgt cccaacagga ggaatcttcc agctccggca ggggcagtcc
     5401 cggagagttc aagtcgaagt gaagtcagtg caggaatctg ggactttacc actgatggaa
     5461 gaatgtatac tgtctgttgg cattggatgt gtcaaagtta gaccgctcag agcccccaga
     5521 acacatgaga ccttccatga ggaagaggaa gacatggaca gctaccagga tcgagattta
     5581 gagagacttc gtagaaaatg gctaaatgca ttaacaaaac gtcaggagta cttggatcaa
     5641 caattgcaaa agcttgtcag taaacgtgat aaaacagagg atgatgctga ccgtgaagcg
     5701 cagcttctgg agatgcggtt gaccctaact gaggagagga acgcggtgat ggtcccctct
     5761 gctggcagtg gtattccagg ggccccagca gaatggaccc cagtacctgg gatggagaca
     5821 cacattcctg ttatattcct ggacttaaat gctgatgatt tcagctctca ggataatctt
     5881 gatgacccag aagctggtgg atgggatgcg accttgactg gggaagaaga agaggagttc
     5941 tttgaattgc agattgtgaa gcagcatgat ggggaggtga aagcagaagc ctcctgggac
     6001 tccgcggtgc atggctgccc tcagctcagc aggggcacgc ccgtggacga gcggttgttc
     6061 ctgatcgtgc gcgtgacggt ccagctcagc caccctgctg acatgcaact ggtgttacgc
     6121 aagagaatct gtgtcaatgt tcacggccgc cagggttttg cacagagtct cctaaaaaag
     6181 atgtctcatc gaagttctat tcctggctgt ggagtgactt ttgaaattgt ctccaatatt
     6241 ccagaggatg cccagggagt ggaagaacgg gaagcattag caagaatggc agccaatgtt
     6301 gaaaacccag cttctgctga ctcggaggct tatattgaaa agtacctcag gagcgtgctg
     6361 gctgtagaaa acctcctgac tttagatcgt ctgcgccagg aagttgcagt gaaggaacag
     6421 ttaacaggaa aaggaaagtt gagcaggagg agtatcagtt ctccaaatgt gaacagattg
     6481 tctggaagcc gacaagatct cattccatca tacagtctag gcagcaacaa gggccggtgg
     6541 gaaagtcagc aggatgtatc ccaaaccaca gtttccagag gaatagctcc tgcccccgcc
     6601 ctctctgttt ctccccaaaa taaccattct ccagatccag gactcagtaa ccttgcagca
     6661 tcctacttga atcctgtcaa atccttcgtg ccgcaaatgc caaagctcct caagtctctc
     6721 tttcccgtcc gcgatgagaa gaggggcaag cggccgtctc ccctcgcaca ccagcccgtg
     6781 ccccgcatca tggtgcagtc agccagcccg gacatcaggg tgaccaggat ggaggaggct
     6841 cagccggaga tgggccctga cgtgctggtg cagacgatgg gggccccggc cttgaagatc
     6901 tgcgacaaac ctgccaaagt gccttcccca ccgcctgtca tagctgtcac agcggtcacc
     6961 ccggctccgg aggcacagga cgggcccccc agccccctga gtgaagcctc tagcgggtac
     7021 ttctcccaca gcgtctccac cgcgaccctg tcggacgccc tgggccccgg cctggacgct
     7081 gcggccccgc cggggtccat gcccaccgcc cctgaggccg agcccgaggc gcccatcagc
     7141 caccccccac cgcccacggc cgtccccgcc gaggagcccc ctggccccca gcagctcgtg
     7201 agccccggtc gggagcgccc cgacctcgag gccccggcgc ccggctcccc gttccgcgtc
     7261 cggagggtgc gggcctcgga gttgcgctcc ttctcgcgca tgctggctgg ggaccccggc
     7321 tgctccccgg gggccgaggg gaatgcgccg gccccgggcg ccgggggaca ggccctggcc
     7381 tctgattccg aggaagctga cgaggtcccg gagtggctcc gagagggcga gttcgtcacc
     7441 gtgggcgccc acaaaacggg cgtggtgaga tacgtggggc ctgccgactt ccaagagggc
     7501 acgtgggtcg gcgtggagct cgacctgccc tcaggtaaga atgacggttc catcggcggg
     7561 aagcagtact tcaggtgtaa ccctggctac gggctgctgg tcaggcccag ccgggtccgc
     7621 agggccacgg gccctgtgcg gcggcgcagc acaggactcc ggctgggtgc ccccgaggcc
     7681 cgccggagcg ccaccctctc gggctccgcc accaacctgg cctcgctgac agctgccctg
     7741 gccaaggccg acaggagcca caagaaccct gagaaccgga aatcctgggc cagcggatcc
     7801 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     7861 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     7921 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     7981 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     8041 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     8101 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     8161 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     8221 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     8281 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     8341 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     8401 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     8461 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     8521 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     8581 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     8641 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     8701 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     8761 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     8821 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     8881 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     8941 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     9001 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     9061 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     9121 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     9181 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     9241 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     9301 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     9361 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     9421 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     9481 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     9541 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     9601 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     9661 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     9721 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     9781 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     9841 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10381 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
    10441 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10501 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
    10561 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10621 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10681 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10741 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    10801 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10861 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10921 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
    10981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    11041 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    11101 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
    11161 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11221 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    12001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    12061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    12181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    12241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    12301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    13021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    13081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    13141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    13201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    13261 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    13321 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    13381 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    13441 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    13501 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    13561 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    13621 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    13681 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    13741 ttgtaaaacg acggccagtg ccaagctg
//
