LOCUS       V027546                11458 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027546
VERSION     V027546
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5484
                     /label="KIF11(NM_004523)"
                     /note="KIF11(NM_004523)"
                     /gene="KIF11"
     CDS             5491..5556
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5600..5811
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5824..6092
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6121..6130
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6151..6813
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6883..6936
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6937..7536
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7537..8125
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8199..8432
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8504..8625
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8665..8800
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8833..8849)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8857..8873
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8881..8911)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8926..8947
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9235..9823)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9994..10854)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10855..10959)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11433..11449
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgtcgcagcc aaattcgtct
     2341 gcgaagaaga aagaggagaa ggggaagaac atccaggtgg tggtgagatg cagaccattt
     2401 aatttggcag agcggaaagc tagcgcccat tcaatagtag aatgtgatcc tgtacgaaaa
     2461 gaagttagtg tacgaactgg aggattggct gacaagagct caaggaaaac atacactttt
     2521 gatatggtgt ttggagcatc tactaaacag attgatgttt accgaagtgt tgtttgtcca
     2581 attctggatg aagttattat gggctataat tgcactatct ttgcgtatgg ccaaactggc
     2641 actggaaaaa cttttacaat ggaaggtgaa aggtcaccta atgaagagta tacctgggaa
     2701 gaggatccct tggctggtat aattccacgt acccttcatc aaatttttga gaaacttact
     2761 gataatggta ctgaattttc agtcaaagtg tctctgttgg agatctataa tgaagagctt
     2821 tttgatcttc ttaatccatc atctgatgtt tctgagagac tacagatgtt tgatgatccc
     2881 cgtaacaaga gaggagtgat aattaaaggt ttagaagaaa ttacagtaca caacaaggat
     2941 gaagtctatc aaattttaga aaagggggca gcaaaaagga caactgcagc tactctgatg
     3001 aatgcatact ctagtcgttc ccactcagtt ttctctgtta caatacatat gaaagaaact
     3061 acgattgatg gagaagagct tgttaaaatc ggaaagttga acttggttga tcttgcagga
     3121 agtgaaaaca ttggccgttc tggagctgtt gataagagag ctcgggaagc tggaaatata
     3181 aatcaatccc tgttgacttt gggaagggtc attactgccc ttgtagaaag aacacctcat
     3241 gttccttatc gagaatctaa actaactaga atcctccagg attctcttgg agggcgtaca
     3301 agaacatcta taattgcaac aatttctcct gcatctctca atcttgagga aactctgagt
     3361 acattggaat atgctcatag agcaaagaac atattgaata agcctgaagt gaatcagaaa
     3421 ctcaccaaaa aagctcttat taaggagtat acggaggaga tagaacgttt aaaacgagat
     3481 cttgctgcag cccgtgagaa aaatggagtg tatatttctg aagaaaattt tagagtcatg
     3541 agtggaaaat taactgttca agaagagcag attgtagaat tgattgaaaa aattggtgct
     3601 gttgaggagg agctgaatag ggttacagag ttgtttatgg ataataaaaa tgaacttgac
     3661 cagtgtaaat ctgacctgca aaataaaaca caagaacttg aaaccactca aaaacatttg
     3721 caagaaacta aattacaact tgttaaagaa gaatatatca catcagcttt ggaaagtact
     3781 gaggagaaac ttcatgatgc tgccagcaag ctgcttaaca cagttgaaga aactacaaaa
     3841 gatgtatctg gtctccattc caaactggat cgtaagaagg cagttgacca acacaatgca
     3901 gaagctcagg atatttttgg caaaaacctg aatagtctgt ttaataatat ggaagaatta
     3961 attaaggatg gcagctcaaa gcaaaaggcc atgctagaag tacataagac cttatttggt
     4021 aatctgctgt cttccagtgt ctctgcatta gataccatta ctacagtagc acttggatct
     4081 ctcacatcta ttccagaaaa tgtgtctact catgtttctc agatttttaa tatgatacta
     4141 aaagaacaat cattagcagc agaaagtaaa actgtactac aggaattgat taatgtactc
     4201 aagactgatc ttctaagttc actggaaatg attttatccc caactgtggt gtctatactg
     4261 aaaatcaata gtcaactaaa gcatattttc aagacttcat tgacagtggc cgataagata
     4321 gaagatcaaa aaaaggaact agatggcttt ctcagtatac tgtgtaacaa tctacatgaa
     4381 ctacaagaaa ataccatttg ttccttggtt gagtcacaaa agcaatgtgg aaacctaact
     4441 gaagacctga agacaataaa gcagacccat tcccaggaac tttgcaagtt aatgaatctt
     4501 tggacagaga gattctgtgc tttggaggaa aagtgtgaaa atatacagaa accacttagt
     4561 agtgtccagg aaaatataca gcagaaatct aaggatatag tcaacaaaat gacttttcac
     4621 agtcaaaaat tttgtgctga ttctgatggc ttctcacagg aactcagaaa ttttaaccaa
     4681 gaaggtacaa aattggttga agaatctgtg aaacactctg ataaactcaa tggcaacctg
     4741 gaaaaaatat ctcaagagac tgaacagaga tgtgaatctc tgaacacaag aacagtttat
     4801 ttttctgaac agtgggtatc ttccttaaat gaaagggaac aggaacttca caacttattg
     4861 gaggttgtaa gccaatgttg tgaggcttca agttcagaca tcactgagaa atcagatgga
     4921 cgtaaggcag ctcatgagaa acagcataac atttttcttg atcagatgac tattgatgaa
     4981 gataaattga tagcacaaaa tctagaactt aatgaaacca taaaaattgg tttgactaag
     5041 cttaattgct ttctggaaca ggatctgaaa ctggatatcc caacaggtac gacaccacag
     5101 aggaaaagtt atttataccc atcaacactg gtaagaactg aaccacgtga acatctcctt
     5161 gatcagctga aaaggaaaca gcctgagctg ttaatgatgc taaactgttc agaaaacaac
     5221 aaagaagaga caattccgga tgtggatgta gaagaggcag ttctggggca gtatactgaa
     5281 gaacctctaa gtcaagagcc atctgtagat gctggtgtgg attgttcatc aattggcggg
     5341 gttccatttt tccagcataa aaaatcacat ggaaaagaca aagaaaacag aggcattaac
     5401 acactggaga ggtctaaagt ggaagaaact acagagcact tggttacaaa gagcagatta
     5461 cctctgcgag cccagatcaa ccttggatcc gactacaaag accatgacgg tgattataaa
     5521 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     5581 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     5641 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5701 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5761 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5821 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5881 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5941 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     6001 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     6061 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     6121 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     6181 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     6241 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     6301 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     6361 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     6421 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     6481 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     6541 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     6601 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     6661 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     6721 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     6781 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     6841 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     6901 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     6961 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7021 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7081 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7141 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7201 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7261 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7321 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7381 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7441 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7501 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     7561 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7621 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7681 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7741 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7801 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7861 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7921 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7981 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8041 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8101 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     8161 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8221 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8281 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8341 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8401 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8461 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8641 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8701 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8761 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
     8821 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    11041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    11101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    11161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    11221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    11281 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11341 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11401 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
