LOCUS       V041323                10786 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041323
VERSION     V041323
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4812
                     /label="KAT2B(NM_003884)"
                     /note="KAT2B(NM_003884)"
                     /gene="KAT2B"
     CDS             4819..4884
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4928..5139
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5152..5420
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5449..5458
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5479..6141
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6211..6264
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6265..6864
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6865..7453
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7527..7760
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7832..7953
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7993..8128
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8161..8177)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8185..8201
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8209..8239)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8254..8275
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8563..9151)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9322..10182)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10183..10287)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10761..10777
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ccgaggctgg cggggccggg
     2341 ccgggcggct gcggggcagg agccggggca ggggccgggc ccggggcgct gcccccgcag
     2401 cctgcggcgc ttccgcccgc gcccccgcag ggctccccct gcgccgctgc cgccgggggc
     2461 tcgggcgcct gcggtccggc gacggcagtg gctgcagcgg gcacggccga aggaccggga
     2521 ggcggtggct cggcccgaat cgccgtgaag aaagcgcaac tacgctccgc tccgcgggcc
     2581 aagaaactgg agaaactcgg agtgtactcc gcctgcaagg ccgaggagtc ttgtaaatgt
     2641 aatggctgga aaaaccctaa cccctcaccc actcccccca gagccgacct gcagcaaata
     2701 attgtcagtc taacagaatc ctgtcggagt tgtagccatg ccctagctgc tcatgtttcc
     2761 cacctggaga atgtgtcaga ggaagaaatg aacagactcc tgggaatagt attggatgtg
     2821 gaatatctct ttacctgtgt ccacaaggaa gaagatgcag ataccaaaca agtttatttc
     2881 tatctattta agctcttgag aaagtctatt ttacaaagag gaaaacctgt ggttgaaggc
     2941 tctttggaaa agaaaccccc atttgaaaaa cctagcattg aacagggtgt gaataacttt
     3001 gtgcagtaca aatttagtca cctgccagca aaagaaaggc aaacaatagt tgagttggca
     3061 aaaatgttcc taaaccgcat caactattgg catctggagg caccatctca acgaagactg
     3121 cgatctccca atgatgatat ttctggatac aaagagaact acacaaggtg gctgtgttac
     3181 tgcaacgtgc cacagttctg cgacagtcta cctcggtacg aaaccacaca ggtgtttggg
     3241 agaacattgc ttcgctcggt cttcactgtt atgaggcgac aactcctgga acaagcaaga
     3301 caggaaaaag ataaactgcc tcttgaaaaa cgaactctaa tcctcactca tttcccaaaa
     3361 tttctgtcca tgctagaaga agaagtatat agtcaaaact ctcccatctg ggatcaggat
     3421 tttctctcag cctcttccag aaccagccag ctaggcatcc aaacagttat caatccacct
     3481 cctgtggctg ggacaatttc atacaattca acctcatctt cccttgagca gccaaacgca
     3541 gggagcagca gtcctgcctg caaagcctct tctggacttg aggcaaaccc aggagaaaag
     3601 aggaaaatga ctgattctca tgttctggag gaggccaaga aaccccgagt tatgggggat
     3661 attccgatgg aattaatcaa cgaggttatg tctaccatca cggaccctgc agcaatgctt
     3721 ggaccagaga ccaattttct gtcagcacac tcggccaggg atgaggcggc aaggttggaa
     3781 gagcgcaggg gtgtaattga atttcacgtg gttggcaatt ccctcaacca gaaaccaaac
     3841 aagaagatcc tgatgtggct ggttggccta cagaacgttt tctcccacca gctgccccga
     3901 atgccaaaag aatacatcac acggctcgtc tttgacccga aacacaaaac ccttgcttta
     3961 attaaagatg gccgtgttat tggtggtatc tgtttccgta tgttcccatc tcaaggattc
     4021 acagagattg tcttctgtgc tgtaacctca aatgagcaag tcaagggcta tggaacacac
     4081 ctgatgaatc atttgaaaga atatcacata aagcatgaca tcctgaactt cctcacatat
     4141 gcagatgaat atgcaattgg atactttaag aaacagggtt tctccaaaga aattaaaata
     4201 cctaaaacca aatatgttgg ctatatcaag gattatgaag gagccacttt aatgggatgt
     4261 gagctaaatc cacggatccc gtacacagaa ttttctgtca tcattaaaaa gcagaaggag
     4321 ataattaaaa aactgattga aagaaaacag gcacaaattc gaaaagttta ccctggactt
     4381 tcatgtttta aagatggagt tcgacagatt cctatagaaa gcattcctgg aattagagag
     4441 acaggctgga aaccgagtgg aaaagagaaa agtaaagagc ccagagaccc tgaccagctt
     4501 tacagcacgc tcaagagcat cctccagcag gtgaagagcc atcaaagcgc ttggcccttc
     4561 atggaacctg tgaagagaac agaagctcca ggatattatg aagttataag gttccccatg
     4621 gatctgaaaa ccatgagtga acgcctcaag aataggtact acgtgtctaa gaaattattc
     4681 atggcagact tacagcgagt ctttaccaat tgcaaagagt acaacccccc tgagagtgaa
     4741 tactacaaat gtgccaatat cctggagaaa ttcttcttca gtaaaattaa ggaagctgga
     4801 ttaattgaca agggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     4861 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     4921 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4981 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5041 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5101 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5161 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     5221 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     5281 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5341 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5401 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     5461 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     5521 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     5581 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     5641 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     5701 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     5761 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     5821 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     5881 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     5941 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     6001 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     6061 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     6121 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     6181 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     6241 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     6301 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6361 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6421 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6481 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6541 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6601 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6661 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6721 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6781 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6841 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     6901 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6961 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7021 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7081 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7141 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7201 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7261 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7321 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7381 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7441 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7501 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     7561 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7741 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7801 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7981 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8041 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8101 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     8161 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8221 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8281 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8341 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9001 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10321 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10381 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10441 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10501 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10561 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10621 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10681 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10741 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
