LOCUS V037269 9247 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037269 VERSION V037269 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..3343 /label="JUNB(NM_002229)" /note="JUNB(NM_002229)" /gene="JUNB" CDS 3344..3361 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" promoter 3406..3617 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3630..3898 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 3927..3936 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 3957..4619 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 4689..4742 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4743..5342 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5343..5930 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6004..6237 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6309..6430 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6449..6584 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6622..6638) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6646..6662 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6670..6700) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6715..6736 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7024..7612) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7783..8643) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8644..8748) /gene="bla" /label="AmpR promoter" primer_bind 9222..9238 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgtgcac taaaatggaa cagcccttct accacgacga 2341 ctcatacaca gctacgggat acggccgggc ccctggtggc ctctctctac acgactacaa 2401 actcctgaaa ccgagcctgg cggtcaacct ggccgacccc taccggagtc tcaaagcgcc 2461 tggggctcgc ggacccggcc cagagggcgg cggtggcggc agctactttt ctggtcaggg 2521 ctcggacacc ggcgcgtctc tcaagctcgc ctcttcggag ctggaacgcc tgattgtccc 2581 caacagcaac ggcgtgatca cgacgacgcc tacacccccg ggacagtact tttacccccg 2641 cgggggtggc agcggtggag gtgcaggggg cgcagggggc ggcgtcaccg aggagcagga 2701 gggcttcgcc gacggctttg tcaaagccct ggacgatctg cacaagatga accacgtgac 2761 accccccaac gtgtccctgg gcgctaccgg ggggcccccg gctgggcccg ggggcgtcta 2821 cgccggcccg gagccacctc ccgtttacac caacctcagc agctactccc cagcctctgc 2881 gtcctcggga ggcgccgggg ctgccgtcgg gaccgggagc tcgtacccga cgaccaccat 2941 cagctacctc ccacacgcgc cgcccttcgc cggtggccac ccggcgcagc tgggcttggg 3001 ccgcggcgcc tccaccttca aggaggaacc gcagaccgtg ccggaggcgc gcagccggga 3061 cgccacgccg ccggtgtccc ccatcaacat ggaagaccaa gagcgcatca aagtggagcg 3121 caagcggctg cggaaccggc tggcggccac caagtgccgg aagcggaagc tggagcgcat 3181 cgcgcgcctg gaggacaagg tgaagacgct caaggccgag aacgcggggc tgtcgagtac 3241 cgccggcctc ctccgggagc aggtggccca gctcaaacag aaggtcatga cccacgtcag 3301 caacggctgt cagctgctgc ttggggtcaa gggacacgcc ttccatcatc accatcacca 3361 ttgagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 3421 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 3481 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 3541 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 3601 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 3661 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 3721 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 3781 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 3841 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 3901 agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg 3961 ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg 4021 gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag 4081 gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact 4141 tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct 4201 acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct 4261 accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg 4321 aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc 4381 accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg 4441 gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca 4501 gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca 4561 acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca 4621 gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg 4681 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc 4741 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 4801 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 4861 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 4921 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 4981 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 5041 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 5101 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 5161 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 5221 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 5281 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 5341 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 5401 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 5461 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 5521 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 5581 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 5641 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 5701 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 5761 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 5821 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 5881 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt 5941 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6001 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 6061 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6121 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6181 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6241 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6301 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6421 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 6481 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 6541 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 6601 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 6661 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6721 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6781 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 6841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7441 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8761 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8821 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 8881 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 8941 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9001 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9061 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9121 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9181 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9241 caagctg //