LOCUS V024755 9308 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024755 VERSION V024755 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..3309 /label="JUN(NM_002228)" /note="JUN(NM_002228)" /gene="JUN" CDS 3316..3342 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3346..3372 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3376..3402 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3467..3678 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3691..3959 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 3988..3997 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4018..4680 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 4750..4803 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4804..5403 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5404..5991 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6065..6298 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6370..6491 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6510..6645 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6683..6699) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6707..6723 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6731..6761) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6776..6797 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7085..7673) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7844..8704) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8705..8809) /gene="bla" /label="AmpR promoter" primer_bind 9283..9299 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ctgcaaagat ggaaacgacc 2341 ttctatgacg atgccctcaa cgcctcgttc ctcccgtccg agagcggacc ttatggctac 2401 agtaacccca agatcctgaa acagagcatg accctgaacc tggccgaccc agtggggagc 2461 ctgaagccgc acctccgcgc caagaactcg gacctcctca cctcgcccga cgtggggctg 2521 ctcaagctgg cgtcgcccga gctggagcgc ctgataatcc agtccagcaa cgggcacatc 2581 accaccacgc cgacccccac ccagttcctg tgccccaaga acgtgacaga tgagcaggag 2641 ggcttcgccg agggcttcgt gcgcgccctg gccgaactgc acagccagaa cacgctgccc 2701 agcgtcacgt cggcggcgca gccggtcaac ggggcaggca tggtggctcc cgcggtagcc 2761 tcggtggcag ggggcagcgg cagcggcggc ttcagcgcca gcctgcacag cgagccgccg 2821 gtctacgcaa acctcagcaa cttcaaccca ggcgcgctga gcagcggcgg cggggcgccc 2881 tcctacggcg cggccggcct ggcctttccc gcgcaacccc agcagcagca gcagccgccg 2941 caccacctgc cccagcagat gcccgtgcag cacccgcggc tgcaggccct gaaggaggag 3001 cctcagacag tgcccgagat gcccggcgag acaccgcccc tgtcccccat cgacatggag 3061 tcccaggagc ggatcaaggc ggagaggaag cgcatgagga accgcatcgc tgcctccaag 3121 tgccgaaaaa ggaagctgga gagaatcgcc cggctggagg aaaaagtgaa aaccttgaaa 3181 gctcagaact cggagctggc gtccacggcc aacatgctca gggaacaggt ggcacagctt 3241 aaacagaaag tcatgaacca cgttaacagt gggtgccaac tcatgctaac gcagcagttg 3301 caaacatttc tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta 3361 cctgattatg ctagctatcc ttatgatgtc ccagactacg catagggatc cctgcgatat 3421 ccagcgcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 3481 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 3541 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 3601 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 3661 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 3721 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 3781 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 3841 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 3901 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 3961 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 4021 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 4081 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 4141 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 4201 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 4261 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 4321 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 4381 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 4441 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 4501 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 4561 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 4621 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 4681 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 4741 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 4801 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 4861 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 4921 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 4981 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 5041 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5101 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5161 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5221 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5281 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5341 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5401 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5461 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5521 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5581 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 5641 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5701 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 5761 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 5821 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5881 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 5941 ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg cctggtacct 6001 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 6061 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 6121 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 6181 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 6241 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 6301 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6361 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6481 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 6541 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 6601 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 6661 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6721 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6781 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6841 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6901 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6961 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 7081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7501 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7621 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7681 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7741 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7801 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7861 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7921 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7981 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 8041 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 8101 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 8161 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8221 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8281 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8341 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8401 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8461 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8521 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8581 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8641 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8701 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8761 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8821 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 8881 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 8941 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 9001 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 9061 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 9121 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 9181 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 9241 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 9301 ccaagctg //