LOCUS       V029045                10521 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029045
VERSION     V029045
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4540
                     /label="JPH3(NM_020655)"
                     /note="JPH3(NM_020655)"
                     /gene="JPH3"
     CDS             4547..4636
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4680..4891
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4904..5172
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5201..5210
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5231..5893
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5963..6016
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6017..6616
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6617..7204
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7278..7511
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7583..7704
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7723..7858
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7896..7912)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7920..7936
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7944..7974)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7989..8010
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8298..8886)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9057..9917)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9918..10022)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10496..10512
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt ccagtggggg caggtttaat tttgacgacg gagggtccta
     2341 ctgtggaggc tgggaggacg gcaaggcgca cggccatggc gtctgcaccg gccccaaggg
     2401 ccaaggcgaa tacaccggct cgtggagcca cggcttcgag gtgctgggcg tctacacctg
     2461 gcccagcggc aacacgtacc agggcacctg ggcgcagggc aagcgccacg gcatcggcct
     2521 ggagagcaag gggaagtggg tgtacaaggg cgagtggacg cacggattca aggggcgcta
     2581 cggggtgcgg gagtgcgcgg gcaacggggc caaatacgaa gggacctgga gcaacgggct
     2641 gcaggacggc tacgggaccg agacctactc ggacggaggg acctaccagg gccagtgggt
     2701 cggtggcatg cgccagggct acggcgtccg gcagagcgtc ccgtatggca tggccgcggt
     2761 catccgctca cccctgagga cgtccatcaa ctccctgcgc agcgagcaca ccaacggcac
     2821 ggcgctgcat cccgacgcct ctccggcggt ggccggcagc ccggccgtgt cccgcggggg
     2881 cttcgtgctc gtggcccaca gtgactccga gatcctcaag agcaagaaga aggggctgtt
     2941 tcggcgctcg ctgctgagtg ggctgaagct gcgcaagtcg gagtccaaga gcagcctggc
     3001 cagccaacgc agcaagcaga gctcctttcg cagcgaggcg ggcatgagca ccgtcagctc
     3061 cacggccagc gacatccact ccaccatcag cctgggcgag gctgaggccg agctggcggt
     3121 catcgaggac gacatcgacg ccaccaccac cgagacctac gtgggcgagt ggaagaacga
     3181 caaacgctcc ggcttcggcg tgagccagcg ctcggacggg ctcaagtacg agggcgagtg
     3241 ggccagcaac cggcgccatg gctacggctg catgaccttc ccggacggca ccaaggagga
     3301 gggcaagtac aagcagaaca tcctcgtcgg cggcaagcgc aagaacctca tccccctgcg
     3361 ggccagcaag atccgcgaga aggtggaccg cgccgttgag gccgctgagc gggccgccac
     3421 catcgccaag cagaaggctg agatcgcggc ttccaggacc tcccactctc gggcaaaggc
     3481 cgaggcagcc ctcacagcag ctcagaaagc ccaggaggag gcgcggatcg ccaggatcac
     3541 tgccaaagag ttctcccctt ccttccagca ccgggaaaac gggctggagt accagaggcc
     3601 gaagcgtcag acctcctgtg acgacatcga ggtgctgtcc accgggacac ccctgcagca
     3661 ggagagcccc gagctgtacc gcaagggcac cactccctcc gacctgaccc ccgacgacag
     3721 ccccctgcag agcttcccca ccagccccgc ggccaccccg ccgcccgcgc ccgccgccag
     3781 gaacaaggtc gcccacttct cgaggcaggt gtcggtggac gaggagcggg gcggggacat
     3841 ccagatgctc ctggagggcc gggccgggga ctgcgcccgc agcagctggg gcgaggagca
     3901 ggccgggggc tccaggggtg tccgcagcgg tgccctgcgc ggcggcctgc tcgtggatga
     3961 cttccgcacc cgaggttcgg gccgcaagca gcccgggaac cccaagccgc gggagcggcg
     4021 gacggagtca ccccccgtgt tcacgtggac ttcccaccac cgggccagca accacagccc
     4081 cggaggctcc aggctgctgg agctgcagga ggagaagctg agcaactacc ggatggagat
     4141 gaaacccttg ctgaggatgg agacgcatcc ccagaaaaga cgctacagca agggcggcgc
     4201 ctgccggggc ttgggggacg accaccgccc cgaggaccgg ggcttcgggg tgcagagact
     4261 gcggtccaag gcccagaaca aggagaactt caggccggcc tcctccgcgg agcccgccgt
     4321 gcagaaactg gcgagcctgc ggctgggcgg ggccgagccc cggttgctgc gttgggactt
     4381 gaccttctcc ccgccccaga aatccttgcc tgtcgctcta gagtccgacg aggagaatgg
     4441 ggatgagctc aagtccagta cgggctcagc gcctatcctg gtggtcatgg tgatcttgct
     4501 caacatcgga gtcgccattc tgtttattaa ctttttcatc ggatccgaac aaaaactaat
     4561 atcagaggaa gatttggaac agaaactaat cagtgaagaa gatttagagc aaaagttaat
     4621 ttccgaggag gacttatagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     4681 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     4741 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4801 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4861 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     4921 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     4981 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     5041 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     5101 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     5161 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga
     5221 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga
     5281 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat
     5341 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta
     5401 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat
     5461 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca
     5521 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg
     5581 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac
     5641 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt
     5701 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag
     5761 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga
     5821 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc
     5881 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg
     5941 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     6001 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6061 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6121 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6181 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6241 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6301 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6361 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6421 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6481 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6541 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6601 caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6661 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6721 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6781 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6841 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6901 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6961 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7021 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7081 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7141 gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc
     7201 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7261 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     7321 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7381 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7441 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7501 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7561 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7621 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7681 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7741 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7801 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7861 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7921 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7981 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8041 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8101 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8161 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8221 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8281 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8341 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8401 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8461 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8521 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8581 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8641 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8701 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8761 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8821 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8881 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8941 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9001 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9061 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9121 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9181 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9241 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9301 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9361 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9421 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9481 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9541 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9601 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9661 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9721 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9781 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9841 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9901 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9961 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10021 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10081 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    10141 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10201 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
    10261 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    10321 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
    10381 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10441 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10501 acgacggcca gtgccaagct g
//
