LOCUS       V044080                11101 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044080
VERSION     V044080
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5955
                     /label="JAG1(NM_000214)"
                     /note="JAG1(NM_000214)"
                     /gene="JAG1"
     CDS             5962..6027
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6071..6282
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6295..6563
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6590..7189
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7196..7784
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7858..8091
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8163..8284
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8303..8438
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8476..8492)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8500..8516
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8524..8554)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8569..8590
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8878..9466)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9637..10497)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10498..10602)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11076..11092
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcgttcc ccacggacgc gcggccggtc cgggcgcccc
     2341 ctaagcctcc tgctcgccct gctctgtgcc ctgcgagcca aggtgtgtgg ggcctcgggt
     2401 cagttcgagt tggagatcct gtccatgcag aacgtgaacg gggagctgca gaacgggaac
     2461 tgctgcggcg gcgcccggaa cccgggagac cgcaagtgca cccgcgacga gtgtgacaca
     2521 tacttcaaag tgtgcctcaa ggagtatcag tcccgcgtca cggccggggg gccctgcagc
     2581 ttcggctcag ggtccacgcc tgtcatcggg ggcaacacct tcaacctcaa ggccagccgc
     2641 ggcaacgacc gcaaccgcat cgtgctgcct ttcagtttcg cctggccgag gtcctatacg
     2701 ttgcttgtgg aggcgtggga ttccagtaat gacaccgttc aacctgacag tattattgaa
     2761 aaggcttctc actcgggcat gatcaacccc agccggcagt ggcagacgct gaagcagaac
     2821 acgggcgttg cccactttga gtatcagatc cgcgtgacct gtgatgacta ctactatggc
     2881 tttggctgca ataagttctg ccgccccaga gatgacttct ttggacacta tgcctgtgac
     2941 cagaatggca acaaaacttg catggaaggc tggatgggcc ccgaatgtaa cagagctatt
     3001 tgccgacaag gctgcagtcc taagcatggg tcttgcaaac tcccaggtga ctgcaggtgc
     3061 cagtacggct ggcaaggcct gtactgtgat aagtgcatcc cacacccggg atgcgtccac
     3121 ggcatctgta atgagccctg gcagtgcctc tgtgagacca actggggcgg ccagctctgt
     3181 gacaaagatc tcaattactg tgggactcat cagccgtgtc tcaacggggg aacttgtagc
     3241 aacacaggcc ctgacaaata tcagtgttcc tgccctgagg ggtattcagg acccaactgt
     3301 gaaattgctg agcacgcctg cctctctgat ccctgtcaca acagaggcag ctgtaaggag
     3361 acctccctgg gctttgagtg tgagtgttcc ccaggctgga ccggccccac atgctctaca
     3421 aacattgatg actgttctcc taataactgt tcccacgggg gcacctgcca ggacctggtt
     3481 aacggattta agtgtgtgtg ccccccacag tggactggga aaacgtgcca gttagatgca
     3541 aatgaatgtg aggccaaacc ttgtgtaaac gccaaatcct gtaagaatct cattgccagc
     3601 tactactgcg actgtcttcc cggctggatg ggtcagaatt gtgacataaa tattaatgac
     3661 tgccttggcc agtgtcagaa tgacgcctcc tgtcgggatt tggttaatgg ttatcgctgt
     3721 atctgtccac ctggctatgc aggcgatcac tgtgagagag acatcgatga atgtgccagc
     3781 aacccctgtt tgaatggggg tcactgtcag aatgaaatca acagattcca gtgtctgtgt
     3841 cccactggtt tctctggaaa cctctgtcag ctggacatcg attattgtga gcctaatccc
     3901 tgccagaacg gtgcccagtg ctacaaccgt gccagtgact atttctgcaa gtgccccgag
     3961 gactatgagg gcaagaactg ctcacacctg aaagaccact gccgcacgac cccctgtgaa
     4021 gtgattgaca gctgcacagt ggccatggct tccaacgaca cacctgaagg ggtgcggtat
     4081 atttcctcca acgtctgtgg tcctcacggg aagtgcaaga gtcagtcggg aggcaaattc
     4141 acctgtgact gtaacaaagg cttcacggga acatactgcc atgaaaatat taatgactgt
     4201 gagagcaacc cttgtagaaa cggtggcact tgcatcgatg gtgtcaactc ctacaagtgc
     4261 atctgtagtg acggctggga gggggcctac tgtgaaacca atattaatga ctgcagccag
     4321 aacccctgcc acaatggggg cacgtgtcgc gacctggtca atgacttcta ctgtgactgt
     4381 aaaaatgggt ggaaaggaaa gacctgccac tcacgtgaca gtcagtgtga tgaggccacg
     4441 tgcaacaacg gtggcacctg ctatgatgag ggggatgctt ttaagtgcat gtgtcctggc
     4501 ggctgggaag gaacaacctg taacatagcc cgaaacagta gctgcctgcc caacccctgc
     4561 cataatgggg gcacatgtgt ggtcaacggc gagtccttta cgtgcgtctg caaggaaggc
     4621 tgggaggggc ccatctgtgc tcagaatacc aatgactgca gccctcatcc ctgttacaac
     4681 agcggcacct gtgtggatgg agacaactgg taccggtgcg aatgtgcccc gggttttgct
     4741 gggcccgact gcagaataaa catcaatgaa tgccagtctt caccttgtgc ctttggagcg
     4801 acctgtgtgg atgagatcaa tggctaccgg tgtgtctgcc ctccagggca cagtggtgcc
     4861 aagtgccagg aagtttcagg gagaccttgc atcaccatgg ggagtgtgat accagatggg
     4921 gccaaatggg atgatgactg taatacctgc cagtgcctga atggacggat cgcctgctca
     4981 aaggtctggt gtggccctcg accttgcctg ctccacaaag ggcacagcga gtgccccagc
     5041 gggcagagct gcatccccat cctggacgac cagtgcttcg tccacccctg cactggtgtg
     5101 ggcgagtgtc ggtcttccag tctccagccg gtgaagacaa agtgcacctc tgactcctat
     5161 taccaggata actgtgcgaa catcacattt acctttaaca aggagatgat gtcaccaggt
     5221 cttactacgg agcacatttg cagtgaattg aggaatttga atattttgaa gaatgtttcc
     5281 gctgaatatt caatctacat cgcttgcgag ccttcccctt cagcgaacaa tgaaatacat
     5341 gtggccattt ctgctgaaga tatacgggat gatgggaacc cgatcaagga aatcactgac
     5401 aaaataatcg atcttgttag taaacgtgat ggaaacagct cgctgattgc tgccgttgca
     5461 gaagtaagag ttcagaggcg gcctctgaag aacagaacag atttccttgt tcccttgctg
     5521 agctctgtct taactgtggc ttggatctgt tgcttggtga cggccttcta ctggtgcctg
     5581 cggaagcggc ggaagccggg cagccacaca cactcagcct ctgaggacaa caccaccaac
     5641 aacgtgcggg agcagctgaa ccagatcaaa aaccccattg agaaacatgg ggccaacacg
     5701 gtccccatca aggattatga gaacaagaac tccaaaatgt ctaaaataag gacacacaat
     5761 tctgaagtag aagaggacga catggacaaa caccagcaga aagcccggtt tgccaagcag
     5821 ccggcgtaca cgctggtaga cagagaagag aagcccccca acggcacgcc gacaaaacac
     5881 ccaaactgga caaacaaaca ggacaacaga gacttggaaa gtgcccagag cttaaaccga
     5941 atggagtaca tcgtaggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     6001 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     6061 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6121 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6181 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6241 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6301 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6361 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6421 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6481 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6541 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta
     6601 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     6661 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6721 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6781 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6841 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6901 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6961 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7021 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     7081 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7141 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca
     7201 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7261 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7321 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7381 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7441 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7501 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7561 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7621 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7681 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7741 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     7801 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7861 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     7921 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7981 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8041 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8101 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8161 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8281 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     8341 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8401 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     8461 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8521 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8581 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8641 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9301 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10021 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10321 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10381 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10441 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10501 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10561 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10621 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10681 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10741 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10801 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10861 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10921 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10981 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    11041 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    11101 g
//
