LOCUS       V036907                10957 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036907
VERSION     V036907
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4983
                     /label="Itih3(NM_008407)"
                     /note="Itih3(NM_008407)"
                     /gene="Itih3"
     CDS             4990..5055
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5099..5310
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5323..5591
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5620..5629
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5650..6312
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6382..6435
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6436..7035
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7036..7624
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7698..7931
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8003..8124
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8164..8299
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8332..8348)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8356..8372
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8380..8410)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8425..8446
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8734..9322)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9493..10353)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10354..10458)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10932..10948
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ggactatgtg gtggccctgc
     2341 cttgtcttgg ctctgctctc tggcttggag acctctggct ttccgagaag tcccctccag
     2401 ctgcttggga aacggagcct cccagagggg gtggtggatg gcattgaggt ttacagcacc
     2461 aagatcagct gcaaggtgac ctcccgcttt gctcacaatg ttgtcaccac aagggctgtc
     2521 aaccgtgcag acacagccaa ggaggtgtcc tttgatgtgg aactgcccaa gacagccttc
     2581 atcaccaact tcaccttgac cattgatggt gtcacctacc ccgggaacgt caaggagaag
     2641 gaagttgccc agaaacaata tgaaaaggct gtgtcccagg gcaagacagc cggtttggtc
     2701 aaggcctctg ggaggaaact ggagaaattc acagtgtctg tcaacgtggc tgcaggcagc
     2761 aaagtcacct ttgagctaac ctatgaagaa ctgctcaaga gaaacaaggg caagtacgag
     2821 atgtacctta aggtccagcc caaacaactg gtcagacact ttgagattga tgcacacatc
     2881 ttcgagccac agggcattag catgctagat gctgaagcct cattcatcac taatgacctc
     2941 ctgggaagcg ccctcaccaa gtccttctca ggcaagaagg ggcacgtgtc tttcaagccc
     3001 agcttagacc agcagcgttc atgcccgacc tgtacagact ccctcctcaa cggggacttc
     3061 accattgtct acgatgtgaa cagagagtct ccagggaatg tgcagatagt caatggctac
     3121 tttgtgcact tctttgcacc ccaaggcctt ccagtggtgc ctaagaacat agtctttgtg
     3181 attgatgtca gcggctccat gtctggtcgg aaaatccagc agaccaggga agcccttctt
     3241 aaaatcctgg atgacgtgaa agaagatgac tacctgaatt tcattctctt cagcaccgat
     3301 gtgaccacct ggaaagatca cctagttcaa gccacccctg caaacctcaa ggaggccaag
     3361 acatttgtga agaacatcca tgatcaaagc atgaccaaca tcaatgatgg gctgctgaag
     3421 ggtattgaaa tgctgaacaa ggcccgagag gaccacactg tcccagagag gagcacctcc
     3481 atcatcatca tgttgacaga cggggatgcc aatactggcg agagcagacc agaaaagatc
     3541 caggaaaacg tccggaatgc catcgggggc aagttccctt tgtataacct gggttttggc
     3601 aacaatctga attataattt cctggagact ctggccctgg agaaccatgg gcttgcccgg
     3661 cgcatttatg aagattctga tgccaacttg cagctgcagg gcttctatga agaggtagct
     3721 aatccactgc tgactaatgt ggaggtggaa taccccgaga acgccatcct agacctcacc
     3781 aggaacagtt acccccactt ctacgatggc tctgagattg ttgtagcagg gcgcctggtg
     3841 gacaggaaca tggacaactt caaagcagat gtgaagggcc atggggcctt gaatgacctg
     3901 accttcacag aggaggtaga catggaggaa atggatgcag ccctgaagga gcaaggctac
     3961 atttttgggg actacattga gcgactctgg gcctacctca ctattgagca gctactggag
     4021 aaacgcaaga atgccaaagg ggatgagaag gagaacatca cagcggaggc cctggatctg
     4081 tccctcaagt accactttgt aaccccactg acctccatgg tggtgaccaa gcctgaggac
     4141 aatgaagacc agacatccat tgctgacaag cctggggaag aagccatcgc tgagaccacg
     4201 accatgtcct tcttgaccac tcagcagtcc agtcagagcc cctactatta tgtggatggg
     4261 gacccccact ttatcatcca aatcccggga aaaaacgaca gcatctgctt caacatcgac
     4321 gagaagcctg gaaccgtgct gcgcctgatt caggacccag tcacaggtat cactgtgacc
     4381 ggacagatca tcggagataa gcgaagtaac gcttcttcca ggacagggaa aacttatttt
     4441 ggcaaactgg gcattaccaa tgcttggatg gacttccggg ttgaggtgac cacggagaaa
     4501 atcatcctag ggactggggc cgagctgagc acgttcagtt ggctagacac aatcacggtc
     4561 acacagactg ggctgtctgt aacaatcaac aggaaaaaga atatggtggt gtcctttgga
     4621 gatgggatta gctttgtgat catcctacac caggtttgga agaaacatcc agttcaccag
     4681 gacttcctag ggttctacgt ggtggacagt caccggatgt cagcacagac acatgggctg
     4741 ctgggccagt tcttccaacc ctttgacttt aaagtgtttg gcatccgccc aggctctgac
     4801 cctacaaagc cagatgccac aatggtggtg aagaatcatc ggttgactgt cacaaggggc
     4861 tcccagaaag attacaggaa ggatgccagt gttggcacca aggtcatctg ctggtttgtc
     4921 cataacaatg gagaaggact aattgatggt gtccataccg actatattgt tcccagcttg
     4981 ttcggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     5041 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     5101 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5161 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5221 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5281 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5341 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5401 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5461 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5521 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5581 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     5641 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     5701 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     5761 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     5821 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     5881 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     5941 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     6001 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     6061 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     6121 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     6181 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     6241 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     6301 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     6361 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6421 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6481 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6541 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6601 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6661 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6721 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6781 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6841 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6901 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6961 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7021 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7081 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7141 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7201 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7261 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7321 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7381 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7441 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7501 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7561 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7621 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7681 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     7741 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7801 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7861 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7921 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7981 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     8041 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8101 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8161 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8221 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8281 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     8341 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8401 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8461 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8521 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10501 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10561 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10621 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10681 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10741 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10801 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10861 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10921 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
