LOCUS       V033025                11379 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033025
VERSION     V033025
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5461
                     /label="Itga5(NM_010577)"
                     /note="Itga5(NM_010577)"
                     /gene="Itga5"
     CDS             5468..5533
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5577..5788
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5801..6069
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6104..6811
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6821..6874
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6875..7474
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7475..8062
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8136..8369
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8441..8562
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8581..8716
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8754..8770)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8778..8794
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8802..8832)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8847..8868
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9156..9744)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9915..10775)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10776..10880)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11354..11370
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggggag ctggacgcca cggtcgcctc gatctcctct
     2341 ccacgcggtg ctgctgcgct gggggccccg acgcctaccg ccgctgctgc ctctgctgct
     2401 gctactgtgg ccgccaccac tccaggttgg gggcttcaac ctagacgcgg aggccccggc
     2461 ggtgctctcc gggccccccg gctccctttt tggcttctcc gtggagtttt accggccggg
     2521 aagggacgga gtcagtgtgc tggttggggc acccaaggct aacactagcc agccaggtgt
     2581 actgcaaggt ggtgctgtct atgtgtgtcc ctggggcacc agtcctatcc agtgcaccac
     2641 cattcaattt gacagcaaag gctcccggat tctggagtcc tcactgtaca gtgccaaggg
     2701 agaggagcct gtggagtaca agtccttgca gtggttcgga gcaacagttc gggcccatgg
     2761 ctcctccatc ttggcatgtg ctccactgta tagctggcgc acagaaaagg acccacagaa
     2821 tgacccagtg ggcacctgct acctctccac agaaaacttc acccggattc tggagtacgc
     2881 accttgccgc tcagattttg gcagtgcagc agggcagggc tactgccaag ggggcttcag
     2941 tgctgagttc accaagactg gccgtgtggt cctgggtgga cctggaagct acttctggca
     3001 aggccagatc ctgtccgcca ctcaagagca gatctcggag tcctattacc cagagtatct
     3061 catcaaccct gttcaggggc agctgcagac ccgccaggcc agctccgtct atgatgacag
     3121 ctacttggga tactctgtgg ctgtgggtga attcagtggt gatgacacag aagactttgt
     3181 tgctggcgtg cccaagggga acctcaccta tggctatgtc accgtcctta atggctcaga
     3241 catccactcc ctctacaacg tctcaggaga acagatggcc tcctacttcg gctatgctgt
     3301 ggctgccact gataccaatg gagatgggct agatgaccta ctggtagggg cacccctgct
     3361 catggagcgg acagctgatg ggagacctca ggaggtgggc agggtctaca tctatctgca
     3421 gcgcccagcg ggcatagatc ccacacccac cctcaccctc actgggcaag atgagttcag
     3481 ccgattcggc agctccttga cacccttggg ggacctggac caagacggct acaatgatgt
     3541 cgccattggg gctccatttg gtggggaggc ccagcaggga gtcgtattta tattcccggg
     3601 aggcccagga ggactgagca ctaaaccttc ccaggttttg cagcctcttt gggcagctgg
     3661 ccgtacccca gacttctttg gctctgccct tcgaggagga cgagatctgg atggcaatgg
     3721 ataccctgat ctaatcgttg gatcctttgg tgtggacaag gctctggtgt acagagggcg
     3781 gcccatcata tctgccagcg catctctcac catcttcccc tccatgttca acccagagga
     3841 gcgcagttgc agcctggaag ggaaccctgt gtcctgcatc aaccttagct tctgcctcaa
     3901 tgcctctgga aaacatgtcc ccaactctat aggcttcgag gtggaactcc aactggactg
     3961 gcagaagcaa aagggagggg tccggcgggc actgttcctg acttccaagc aggccaccct
     4021 tacccagacc ctgcttatcc agaatggggc tcgggaggac tgcagggaga tgaagatcta
     4081 cctcaggaat gaatcagaat tcagagacaa actctcccca attcacattg ccctcaactt
     4141 ctccttggac cccaaagctc ccatggacag ccatggcctc cggccagttc tacactacca
     4201 aagcaaaagc aggatagagg acaaggccca gatcttgctg gactgtggtg aagacaatat
     4261 ctgtgtgcct gacctgcagc tggatgtgta tggggagaag aaacatgtgt acctgggtga
     4321 caagaacgca ctgaacctga cattccatgc ccaaaatctg ggtgagggcg gtgcctatga
     4381 agccgagctt cgggtcacag cccctctaga ggccgagtac tcaggacttg tcagacaccc
     4441 agggaacttc tccagcctga gctgtgacta ctttgctgtg aaccagagcc gccagctggt
     4501 gtgtgacctg ggcaacccca tgaaggcagg caccagtctc tggggtggcc ttcggttcac
     4561 tgttcctcat cttcaagaca caaagaaaac catccagttt gactttcaga tcctcagcaa
     4621 gaacctgaac aactcacaaa gcaacgtggt ctccttccca ctctcggtgg aggctcaagc
     4681 ccaggtctcc cttaatggtg tctccaagcc tgaagctgtg attttcccag tcagcgactg
     4741 gaatcctcaa gaccagcctc agaaggagga agacttgggc ccagctgtcc accatgtcta
     4801 cgagctcatc aaccaggggc ccagctccat cagccagggt gtgctggagc tcagctgtcc
     4861 acaggctctg gaaggccaac agctcctcta tgtgaccaag gtgacaggac tcagcaactg
     4921 cacctccaac tacaccccca actcacaggg cctggagttg gatccagaga cctctccaca
     4981 ccacctgcag aaacgagagg ctccagggag gagttctact gcctcaggaa cacaagttct
     5041 gaaatgccct gaagccaagt gtttcaggct gcgctgtgag tttgggccac tgcaccgcca
     5101 agagagccgt agtctgcagc tgcatttccg agtctgggcc aagaccttct tgcagcggga
     5161 ataccagcca tttagccttc agtgtgaggc tgtatatgaa gctctgaaga tgccctacca
     5221 gatcctgcct cggcagcttc cccaaaagaa acttcaggtg gccacagccg tgcagtggac
     5281 caaggcagaa ggcagcaatg gtgtcccgtt gtggatcatc atcctagcca ttctttttgg
     5341 cctcctgctc ctaggtctgc tcatctacgt cctctacaag ctcggcttct tcaaacgttc
     5401 cctcccctac ggcacagcca tggaaaaagc tcagctcaag cctccagcca cctcagatgc
     5461 cggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     5521 tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5581 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5641 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5701 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5761 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5821 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5881 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5941 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     6001 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     6061 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga
     6121 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     6181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6241 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     6301 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     6361 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6421 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6481 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     6541 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     6601 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     6661 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     6721 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6781 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct
     6841 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     6901 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6961 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7021 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7081 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7141 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7201 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7261 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7321 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7381 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7441 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     7501 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7561 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7621 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7681 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7741 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7801 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7861 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7921 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7981 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     8041 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     8101 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8161 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8221 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8281 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8341 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8401 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8461 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8521 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8581 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8641 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8701 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     8761 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8821 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8881 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8941 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10921 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10981 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    11041 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    11101 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11161 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    11221 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    11281 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    11341 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
