LOCUS       V040308                11827 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040308
VERSION     V040308
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5853
                     /label="Itga1(NM_001033228)"
                     /note="Itga1(NM_001033228)"
                     /gene="Itga1"
     CDS             5860..5925
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5969..6180
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6193..6461
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6490..6499
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6520..7182
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7252..7305
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7306..7905
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7906..8494
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8568..8801
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8873..8994
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9034..9169
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9202..9218)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9226..9242
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9250..9280)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9295..9316
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9604..10192)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10363..11223)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11224..11328)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11802..11818
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tccccaggcg tcctgctagc
     2341 ctagaggtca ctgtagcctg catctggctt ctcaccgtta tcctaggcgt ctgcatctcc
     2401 ttcaacgttg atgtcaaaaa ctccatgagc ttcagtggtc cagtggagga catgtttgga
     2461 tacaccgttc aacagtatga aaacgaagaa ggcaaatggg ttcttattgg ttctccttta
     2521 gttggccaac ccaaagcaag aactggagat gtctataagt gtccagttgg gagagagaga
     2581 tcaatgcctt gtgtgaagtt ggatttacca gttaacacat cgattcctaa tgtcacagaa
     2641 ataaaggaaa acatgacatt tggatcaact ttagtcacca atccgaaggg aggatttctg
     2701 gcatgtgggc ccttgtatgc ctatagatgt ggacatttgc attatacgac tggcatatgt
     2761 tctgatgtca gccctacatt tcaagttgtg aactcgttcg cccctgtaca agaatgtagc
     2821 acacagcttg acatagtcat cgtcctagat ggctccaaca gcatctaccc ctgggaaagt
     2881 gtcactgcct ttttaaacga ccttcttaag aggatggaca ttggccccaa acagacacag
     2941 gttggaattg tacaatatgg agcaaatgta acacatgagt tcaacctcaa taagtattca
     3001 tccacagaag aggtcctggt ggcagcaaac aaaataggcc gcaggggtgg tctccagacg
     3061 atgacagccc ttggaataga cacagccagg aaagaggcat tcactgaagc tcggggggcc
     3121 agaaggggag tgaaaaaagt catggttatt gtgactgatg gagaatcgca tgacaactat
     3181 cgcctgaaac aggtcatcca agactgtgag gatgaaaaca ttcagcgatt ttccatagct
     3241 atccttggcc actataacag ggggaactta agcaccgaaa aatttgtgga ggagataaaa
     3301 tcaatcgcaa gtgagcccac cgaaaagcac ttcttcaacg tctcagatga gttggccctg
     3361 gtcactattg ttaaagctct gggagaaagg atatttgctt tggaagctac agctgaccag
     3421 tcagcagctt cgtttgaaat ggagatgtct cagaccggct tcagtgctca ttattcacag
     3481 gactgggtca tgcttggagc tgttggagcc tatgactgga acggaactgt ggtcatgcag
     3541 aaggctaacc agattgtcat ccctcataac accacctttc aaactgagcc caccaagatg
     3601 aacgagcctc ttgcttctta cttgggttac acagtgaact ctgccaccat cccgggagat
     3661 gtgctctata tcgctgggca gcctcggtac aatcacaccg gccaggtcgt catctacaag
     3721 atggaggatg gggacgtcaa cattctgcag acactcagtg gagagcagat cggatcctac
     3781 tttggtagcg tcttaaccac aattgacatc gacaaagatt cttacactga tctgcttctc
     3841 gtcggggctc ccatgtatat ggggacagag aaagaggaac agggcaaggt gtacgtgtac
     3901 gctgtgaatc agacgaggtt tgaatatcaa atgagcctgg aaccaattaa gcagacctgc
     3961 tgttcatccc tgaaggataa ttcatgcaca aaagaaaaca agaatgagcc ctgtggggcc
     4021 cgttttggaa cagcagttgc tgctgtaaaa gacctcaacg tggatggctt taatgacgtc
     4081 gtgattggag ctcccctgga agacgaccac gcaggagctg tgtacattta tcatggcagt
     4141 ggcaagacca taaggaaaga gtatgcgcaa cgcattccct caggtgggga tgggaagacg
     4201 ctgaaatttt ttggccagtc tatccacgga gagatggatt taaatggcga tggtctgact
     4261 gacgtgacca ttggaggcct tggaggagct gccctcttct gggccagaga tgtggctgta
     4321 gttaaagtga ccatgaattt tgaacccaac aaagtgaata ttcaaaagaa aaactgccgt
     4381 gtggagggca aagaaacagt atgcataaat gctacaatgt gttttcatgt gaaattgaag
     4441 tctaaagaag actcagttta cgaggctgat ctgcagtacc gtgtcaccct tgattcgctg
     4501 aggcagatat cacggagctt tttttctgga actcaggaaa ggaggattca aagaaacctc
     4561 accgttcgag aatccgaatg catcaggcac tccttctaca tgttggataa gcacgacttt
     4621 caggactccg tgagagtgac tttggatttt aacctcactg atccagaaaa tgggcccgtg
     4681 ctcgatgacg ctctgccaaa ctcagtccat ggacatattc cttttgccaa agactgtggg
     4741 aacaaggaaa gatgcgtttc agacctcacc ctggatgtgt ccacaacaga aaagaacctg
     4801 ctgattgtca gatcccagaa tgacaagttc aatgtcagcc tcaccgtcaa aaacaaggga
     4861 gacagtgcgt acaacacccg gacagtggtt cagtattctc caaatctgat tttttcagga
     4921 attgaggaga tccaaaaaga tagctgcgaa tccaatcaaa atatcacgtg tagagttgga
     4981 tatcctttcc tgaggacagg agacgtggtt aacttcaaaa taatattcca gtttaataca
     5041 tcacatctct cagaaaatgc aattattcat ttaagtgcaa caagtgacag tgaagagccc
     5101 ctggaatctc tttacgataa tgaagtaaat atttccatcc ccgtaaaata tgaagtcgga
     5161 ctgcagtttt acagttctgc gagtgaacac cacatttcag ttgctgccaa tgagactgtt
     5221 cctgagctga ttaattccac caaggacatt ggggatgaaa ttaatgtctt ctacacgatt
     5281 agaaaaagag ggcatttccc aatgccagaa cttcggctgg caatttcatt tcccaatctg
     5341 acgtcagatg gctatcctgt actgtaccca actggatggt catcttctga taatgtaaac
     5401 tgcagacctc gaagccttga ggaccctttg ggtatcaact ctgggaagaa aatgacaata
     5461 tcaaagtctg aggttctcaa aagaggcaca atccaggact gcagtacctg caagattgct
     5521 accatcacgt gtcatctcct tccctcggat gtgagtcaag tgaatgtctc actcatcttg
     5581 tggaaaccaa ctttcataaa agcacatttt tccagcttaa atcttaccat acggggagaa
     5641 cttcagagtg aaaactcatc gctgacttta agcagcagca accggaaacg agagctggct
     5701 attcagatat ccaaagacgg gctcccaggc cgagtgccgc tgtgggttat cctcctgagc
     5761 gcctttgcgg gactcctgct gttaatgctg cttatattag ctctgtggaa gattggattc
     5821 ttcaaaaggc cactaaagaa gaaaatggag aaaggatccg actacaaaga ccatgacggt
     5881 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5941 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6001 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     6061 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6121 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6181 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6241 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6301 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6361 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6421 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6481 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     6541 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6601 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6661 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6721 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6781 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6841 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6901 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6961 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     7021 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     7081 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     7141 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     7201 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     7261 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     7321 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7381 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7441 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7501 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7561 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7621 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7681 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7741 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7801 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7861 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7921 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7981 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8041 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8101 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8161 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8221 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8281 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8341 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8401 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8461 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     8521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8581 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8821 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8881 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8941 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9001 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     9181 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9241 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9301 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9361 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9421 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10021 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    10081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11341 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11401 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    11461 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    11521 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    11581 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11641 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11701 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11761 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11821 caagctg
//
