LOCUS       V028743                11179 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028743
VERSION     V028743
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5205
                     /label="Ireb2(NM_022655)"
                     /note="Ireb2(NM_022655)"
                     /gene="Ireb2"
     CDS             5212..5277
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5321..5532
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5545..5813
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5842..5851
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5872..6534
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6604..6657
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6658..7257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7258..7846
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7920..8153
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8225..8346
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8386..8521
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8554..8570)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8578..8594
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8602..8632)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8647..8668
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8956..9544)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9715..10575)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10576..10680)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11154..11170
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg actccccaag tgcaggatac
     2341 acctttgagt accttattga aacattaaat ggcaattcac agaagaagtt cttcaatgta
     2401 cctaaacttg gcggcaccaa gtatgatatt ctgccttact caatacgggt cctattggaa
     2461 gctgctgtac gaaattgtga tggcttttta atgaaaaagg aagatgttat gaacattttg
     2521 gactggaaaa ccaaacagag caatgttgaa gtgccctttt tccctgcccg tgttgttctt
     2581 caagatttca ctggaatacc ggcaatggtg gattttgctg ctatgaggga ggcagtgaaa
     2641 actcttggag gtgatcctaa gaaagtccac cctgcctgtc cgacagatct cacagtggac
     2701 cactctttac agattgactt cagtaaatgt gcaatacaga atgcaccaaa tcctggaggt
     2761 ggtgacctac agaaagcagg aaagctctct ccacttaaag tgcagtctaa gaagcttcca
     2821 tgtcgaggcc agactacctg ccggggatcg tgtgattctg gagagctaag ccggaactca
     2881 ggaacatttt cttcacagat tgagaataca cctgtcttgt gtccttttca tttgcaacca
     2941 gtgcctgaac ctgagacagt gttaaaaaat caagaagtag aatttggcag aaatcgagag
     3001 aggcttcagt ttttcaagtg gagctcagga gcttttaaga atgtggcagt catcccgcct
     3061 ggaactggaa tggctcatca agtgaactta gaatatttat caagagtagt ttttgaagaa
     3121 acggacctgc tcttcccaga cagtgtagtt ggcacagatt ctcatataac catggtgaac
     3181 ggattgggga ttcttgggtg gggagttggt ggcattgaga cagaggcagt tatgcttggc
     3241 ctgccagtta ctcttacttt accagaggtg gttggatgtg agctaactgg gtcatccaat
     3301 gcttttgtta catccataga tattgtcctt ggcattacaa agcacctcag gcaagtaggc
     3361 gtggctggaa agtttgttga gttctttgga agtggagttt cacaattatc tattgtcgat
     3421 agaactacca tagcaaacat gtgtccagaa tatggtgcta tcctcagctt tttccctgtt
     3481 gacaatgtga cactacgaca tttagaacat acaggttttg acaaaaccaa acttgagtca
     3541 atggaaaaat accttaaagc tgtgaaattg tttcgaaatg atgagaattc ttcagaacct
     3601 gaatattctc aggtgataca gattaatctg aattcaatag ttgcatctgt cagtggtcca
     3661 aaaaggcctc aggatagggt tgctgtaaca gatatgaaaa gtgattttca ggcttgctta
     3721 aatgaaaagg ttggatttaa aggcttccaa gttgcagcag aaaaacaaag tgacactgtc
     3781 tctgttcgtt atgatggaag tgagtataag ctgtcccatg gatccgtggt cattgctgca
     3841 gttatcagtt gtaccaataa ttgcaatcca tctgtgatgc ttgctgcagg tcttctggct
     3901 aaaaaggctg ttgaaattgg tctacgagtt aaaccttata tacgaacaag tttgtctcca
     3961 ggcagtggga tggttacaca ttacctcagt tcaagtggag tgctacccta tcttagcaag
     4021 ctagggtttg acatagttgg ctatggatgt tcaacatgtg taggaaatac agcaccttta
     4081 tcagaagcag ttttgaatgc agtaaaacag ggtgatttgg ttacctgtgg agttttatct
     4141 ggaaacaaac atttcgaagg ccgtctctgt gattgtgtcc gtgccaatta tctcgcctct
     4201 ccgcccttag tggtggctta tgccatagca ggcacagtga atatagactt ccagacagag
     4261 cctttaggta ctgactctac gggcaaggaa atttacctgc atgacatttg gcctagtcga
     4321 gaagaagttc atcagatgga ggaagaacat gttatattgt caatgtttaa aacactaaaa
     4381 gagaaagtag agatgggaaa taaacggtgg aattcgttag aagctccaga ctccgtgctg
     4441 tttccatggg atgtgaagtc tacttacatc agatgccctt cattttttga taagcttacc
     4501 aaagagccag ctgcatccca gcctattgag aatgcccatg tcctgttgta tttgggagac
     4561 tctgtcacta cagatcatat atcacccgct ggaagtattg ctaggagtag tgctgctgct
     4621 aagtatttga caaacagagg ccttactccc cgagagttca actcttatgg agctcgaaga
     4681 ggtaatgatg ctgtgatgac aagaggcaca tttgcaaaca tcaagctttt taataagttt
     4741 attgggaagc cagctcccaa aacaattcat tttccatcgg gacaaacgct cgatgtattt
     4801 gaagctgcag aactgtacca gaaagaaggt atcccactga taattttagc aggaaaaaaa
     4861 tatggttcag gaaattcaag agattgggct gccaagggac cctatttgct gggtgtaaaa
     4921 gctgttttgg ctgagagcta tgaaaaaata cacaaagatc atttgattgg aattggtata
     4981 gcaccacttg aattcctccc aggagaaaat gcagattcct tgggcctctc tggcagagaa
     5041 gtattttctt tatcatttcc tgaagaactc tttcctggaa ttacattaaa tataaagaca
     5101 agcactggaa aagagttcag cgtgattgca tcatttgcaa atgacgtgga gataactttg
     5161 tacaaacatg gaggcttatt aaactttgtg gctcgaaaat tcttaggatc cgactacaaa
     5221 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     5281 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5341 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5401 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5461 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5521 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5581 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5641 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5701 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5761 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5821 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     5881 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     5941 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6001 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6061 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6121 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     6181 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     6241 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     6301 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6361 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6421 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6481 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     6541 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     6601 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6661 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6721 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6781 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6841 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6901 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6961 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7021 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7081 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7141 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7201 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     7261 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7321 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7381 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7441 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7501 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7561 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7621 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7681 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7741 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7801 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     7861 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7921 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     7981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8161 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8221 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8341 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8461 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8521 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     8581 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8641 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8701 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8761 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8821 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8881 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8941 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9001 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9061 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9121 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9181 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9241 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9301 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9361 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9421 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9481 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9601 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9661 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9721 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9781 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9841 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9901 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9961 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10021 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10081 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10141 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10201 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10261 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10321 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10381 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10441 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10501 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10561 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10621 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10681 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10741 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10801 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10861 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10921 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10981 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11041 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11101 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11161 gacggccagt gccaagctg
//
