LOCUS       V029253                13261 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029253
VERSION     V029253
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7287
                     /label="Iqgap1(NM_016721)"
                     /note="Iqgap1(NM_016721)"
                     /gene="Iqgap1"
     CDS             7294..7359
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7403..7614
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7627..7895
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7924..7933
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7954..8616
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8686..8739
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8740..9339
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9340..9928
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10002..10235
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10307..10428
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10468..10603
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10636..10652)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10660..10676
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10684..10714)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10729..10750
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11038..11626)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11797..12657)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12658..12762)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13236..13252
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ccgccgcgga ggaggttgat
     2341 ggcctgggtg tggtccggcc gcactatggc tccgtcctgg ataatgagag gctcactgca
     2401 gaggagatgg atgagcggag acggcagaac gtggcttatg aatacctttg tcatctggaa
     2461 gaagcaaaga ggtggatgga agcatgccta ggtgaggacc tgccgcccac cacagagcta
     2521 gaggagggcc ttagaaacgg agtctacctt gccaagctag ggaacttctt ctctcccaaa
     2581 gtggtgtccc tgaagaaaat ctatgatcga gaacagacca gatacaaggc taccggcctc
     2641 cacttcagac acacggataa tgtgattcag tggctgaatg ccatggatga gattgggttg
     2701 cctaagattt tttacccaga aaccacagat atctatgacc ggaagaacat gccaagatgc
     2761 atctactgta tccacgccct cagtttgtac ctgttcaaac tgggcctggc tcctcagatt
     2821 caagacctgt atggaaaggt tgatttcaca gaagaagaaa tcaacaacat gaagatcgag
     2881 ctggagaagt acgggatcca gatgcctgcc ttcagcaaga tcgggggcat cctggctaat
     2941 gagctctcag tggatgaagc tgcgctacat gctgctgtta ttgctattaa tgaagcgatt
     3001 gatcgcagag ttgcagctga cacttttacg gctctaaaaa accccaatgc catgctcgtc
     3061 aatcttgaag aaggcctggc tcccacgtac caagacgtgc tttaccaggc caagcaggac
     3121 aagatgacaa acgctaaaaa caggacggaa aactctgaca gagaaaggga cgtttatgag
     3181 gagctgctca cacaagctga aatccaaggg aatgtaaaca aagtcaacac atcttctgcc
     3241 ctggccaaca tcagcctggc tttagagcag ggctgtgcag tgaccctgct caaggctctg
     3301 cagtcactgg ctctgggcct ccgagggctg cagacccaga acagcgactg gtacatgaag
     3361 cagctacaga gtgatctgca gcaaaagaga cagagtggcc agactgaccc cctgcagaag
     3421 gaggaggtac aggccggagt ggatgctgcc aacagtgctg cccagcagta ccaacgacgg
     3481 ttggcagcag tggcagcaat caacgctgcc atccagaagg gcatcgctga gaagaccgtg
     3541 ttggagctaa tgaatcctga agcccagctg ccccaggtgt atccatttgc agctgatctc
     3601 tatcagaagg agttggccac cctgcagcag cagagccctg agcatagcct cacccatcct
     3661 gagctcactg ttgctgtgga gatgctgtca tccgtggccc tcatcaacag ggcgctggag
     3721 tcaggagaca tgaccactgt gtggaagcag ctgagcagct cagttacggg ccttaccaac
     3781 atcgaggaag aaaactgtca aaggtatctc gatgagctga tgaagctgaa ggctcaggca
     3841 catgccgaga ataatgcatt tattacatgg aatgacatcc aggcgtgtgt ggaccatgtg
     3901 aacctggtgg tccatgagga gcatgagcgg attttggcca tcggcttgat taatgaagcc
     3961 ctggatgaag gggacgctca gaagactctg caggccctgc agatccctgc agccaagctc
     4021 gagggcgtcc ttgcagaagt ggcacagcac tatcaagaca cgctgatcag agcaaagaga
     4081 gaaaaggccc aggaaacaca ggatgagtca gctgtgttat ggttggatga aattcaaggt
     4141 ggaatctggc agtccaacaa agacacccaa gaggcccaga ggtttgcctt aggaatctct
     4201 gccatcaatg aagcagtaga cagcggtgat gttggcagaa ccctgagtgc cctacgttct
     4261 cccgacgttg gcttatatgg agtgatcccc gaatgtgggg aaacgtacca gagtgacctt
     4321 gctgaagcca agaagaagag actggcagca ggagataata acagcaagtg ggtgaagcac
     4381 tgggtgaaag gcgggtacca ttactaccac aacctggaga cgcaagcagg aggatgggct
     4441 gagcccccag actttgtgca gaattctgtg cagctttctc gagaggagat ccagagctcc
     4501 atctctggag taaccgctgc atataaccga gagcagcttt ggctggccaa cgaaggcttg
     4561 atcaccaagc tgcaagcctg ctgccgtggg tacctcgttc gacaggaatt ccgatcccgg
     4621 atgaattttc tgaagaaaca gatccctgcc atcacctgca ttcagtcaca gtggagagga
     4681 tacaaacaga agaaggcata tcaagatcgg ctggcttacc tgcactccca taaagacgaa
     4741 gttgtgaaga ttcagtccct tgccaggatg catcaagctc gaaagcgcta tagagatcgc
     4801 ctacagtatt tccgagacca tataaatgac attatcaaaa tccaggcttt cattcgggcc
     4861 aacaaagctc gtgatgacta caagactctc atcaatgctg aggacccgcc tatgattgtg
     4921 gtccgaaagt ttgtccacct cctggaccaa agtgatcagg acttccagga ggaacttgat
     4981 ctcatgaaga tgcgcgagga ggtcatcacc ctcatccgtt ccaaccagca gctggagaac
     5041 gacctcaacc tcatggatat caaaatcgga ctgctggtga agaacaagat cacgctgcag
     5101 gatgtggttt cccatagtaa aaaacttacc aaaaaaaata aggaacagct gtccgacatg
     5161 atgatgataa acaagcagaa gggcgggctc aaggctttga gcaaagagaa gagggagaag
     5221 ctggaggcct atcagcatct cttttatctc ctgcagacca accctaccta tctggccaag
     5281 ctgatctttc agatgccaca aaacaagtcc accaaattca tggactctgt gatcttcacg
     5341 ctgtacaact atgcatctaa ccagcgggag gagtacctgc tgctgcggct cttccagaca
     5401 gctctgcagg aggagatcaa gtcaaaggtg gatcagattc aagaaatcgt gacaggaaac
     5461 cctacggtta ttaagatggt tgtaagtttc aaccgtggtg cccggggcca gaatgccctc
     5521 cggcagatct tggcccctgt cgtgaaggaa attatggatg acaagtctct caacatcaaa
     5581 accgaccctg tggatattta caagtcttgg gttaatcaga tggagtcgca gacaggagag
     5641 gcgagcaaac tgccctatga tgtgactcct gaacaagcct tgtctcatga agaagtgaag
     5701 acgaggttag acaactccat caggaacatg agggccgtga cagacaagtt cctctcagcc
     5761 atcgtcagct ctgtggacaa aatcccttat gggatgcgct tcattgccaa agtcctgaag
     5821 gattccctgc acgagaagtt ccctgacgct ggtgaggacg agctgctgaa gattatcggt
     5881 aacctgcttt actaccgata catgaaccca gccatcgtcg ctcccgatgc cttcgacatc
     5941 attgacctgt cagcaggggg ccagctcacc acagaccagc gcagaaacct gggctccatt
     6001 gccaagatgc tccagcacgc ggcgtccaac aagatgtttc tgggcgataa tgcccactta
     6061 agcatcatta atgagtatct ctcgcagtcc taccagaaat tcagacggtt tttccaagtg
     6121 gcttgtgacg tcccagagct gcaggataaa tttaacgtgg atgagtactc tgacctagtc
     6181 accctcacta agccagttat ctacatctcc attggcgaaa tcatcaacac ccacactctc
     6241 ctgttggacc atcaggatgc cattgctcca gagcataacg accccatcca cgaacttctg
     6301 gacgaccttg gggaggtgcc caccattgag tcccttatag gagaaagctg tggcaattca
     6361 aacgacccca acaaggaggc tctggctaag acggaagtgt ctctcacgtt gaccaacaag
     6421 tttgacgtgc ctggtgacga gaacgcagag atggacgctc ggaccatctt actgaataca
     6481 aaacgtttaa ttgtggatgt catccggttc cagccaggag agaccttgac tgaaattcta
     6541 gaaaccccag ccaccaatga acaggaagct gaacatcaga gggccatgca gagacgggct
     6601 atccgcgatg ccaaaacccc tgacaagatg aaaaaatcaa agcccatgaa ggaggataac
     6661 aacctcagcc tccaggagaa gaaagagaag atccagactg gcctaaagaa gctaacggag
     6721 cttgggacgg tggacccaaa gaacagatac caggaactca tcaacgacat tgccaaggat
     6781 atccggaatc agcggagata caggcagagg aggaaagctg aattggtaaa actgcagcag
     6841 acgtactcgg cgctgaactc taaggccacc ttttacggcg agcaggtgga ctactacaag
     6901 agctacatca aaacctgctt ggataacttg gccagcaagg gcaaggtctc caaaaagcct
     6961 agggaaatga aaggcaagaa aagcaaaaag atttctctga agtacacagc agcgaggctg
     7021 catgagaagg gcgtccttct ggagattgaa gaccttcagg caaaccaatt taaaaatgtt
     7081 atcttcgaaa ttggtccaac agaagaagtt ggagactttg aagtaaaagc caagttcatg
     7141 ggagttcaga tggagacttt catgttgcat tatcaggact tgctgcagct acagtatgaa
     7201 ggagttgcag ttatgaaatt atttgataga gctaaagtga atgtcaacct cctgatcttc
     7261 cttctcaaca aaaagttcta tgggaaggga tccgactaca aagaccatga cggtgattat
     7321 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     7381 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     7441 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     7501 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     7561 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     7621 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     7681 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     7741 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     7801 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     7861 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     7921 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     7981 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     8041 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     8101 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     8161 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     8221 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     8281 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     8341 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     8401 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     8461 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     8521 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     8581 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     8641 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     8701 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     8761 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8821 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     9001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     9061 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     9121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     9181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     9241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     9301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     9361 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     9421 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     9481 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     9541 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     9601 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     9661 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9721 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     9781 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9841 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9901 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     9961 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    10021 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
    10081 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10141 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10201 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    10261 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
    10321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
    10441 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10501 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10561 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
    10621 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10681 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11461 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11581 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11641 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11701 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12661 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12721 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12781 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    12841 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    12901 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    12961 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    13021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    13081 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    13141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    13201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    13261 g
//
