LOCUS       V045745                11570 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045745
VERSION     V045745
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5607
                     /label="Ipo5(NM_023579)"
                     /note="Ipo5(NM_023579)"
                     /gene="Ipo5"
     CDS             5608..5634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5638..5664
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5729..5940
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5953..6221
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6250..6259
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6280..6942
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7012..7065
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7066..7665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7666..8253
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8327..8560
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8632..8753
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8772..8907
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8945..8961)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8969..8985
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8993..9023)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9038..9059
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9347..9935)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10106..10966)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10967..11071)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11545..11561
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcggccgc ggcggagcag
     2341 caacagttct acctgctcct gggaaacctg ctcagccccg acaatgtggt ccggaaacag
     2401 gcggaggaaa cctatgagaa tatcccaggc cggtccaaga tcacattcct cttacaagcc
     2461 atcagaaaca caaccgctgc cgaggaggct agacaaatgg ctgctgttct cctaagacgt
     2521 cttttgtcct ctgcatttga tgaagtctac ccagctcttc catcagatgt ccagactgcc
     2581 atcaagagtg aattgctaat gatcattcag atggaaacac aatccagcat gaggaagaaa
     2641 atctgtgata ttgctgcaga attggccagg aatttaatag atgaggacgg caacaaccag
     2701 tggcctgaag gtttgaagtt cctctttgat tcagtcagct cacaaaacat gggactccgg
     2761 gaagctgccc ttcacatatt ttggaacttt cctggaattt ttgggaacca acaacagcac
     2821 tatttggacg tcatcaaacg gatgttagtt cagtgtatgc aagatcagga gcatccttcg
     2881 atcaggacac tgtctgccag agctactgct gccttcatac ttgccaatga gcataatgtc
     2941 gctctgttca aacactttgc agacttgctg cctggattct tacaggctgt caatgactca
     3001 tgctaccaga atgatgactc ggtcctaaaa tcccttgttg agattgcaga caccgtacca
     3061 aagtacttgc gccctcactt agaagcaact ctgcagttga gtctgaagtt gtgtggagac
     3121 actaacctca ataatatgca gcgccagctt gcccttgaag tgattgtgac actgtcagag
     3181 actgcagctg ctatgttaag aaagcatacc agtctcatcg cacagacgat tcctcagatg
     3241 ttggcaatga tggttgacct agaagaagat gaggactggg caaatgctga tgagctagaa
     3301 gatgatgatt tcgatagcaa tgcagttgct ggtgagagtg cattagaccg aatggcctgt
     3361 ggacttggtg gaaagctcgt tctgcccatg attaaggaac acattatgca aatgcttcaa
     3421 aaccctgatt ggaaatacag gcatgcagga ctgatggccc tatccgccat tggcgaagga
     3481 tgccaccagc agatggaagg aattctaaat gagattgtaa attttgtctt gcttttcctc
     3541 caggatcctc atccgagagt ccggtatgca gcctgcaatg ctgtgggtca aatggctaca
     3601 gattttgcac ctggttttca aaagaaattt catgagaagg tgattgcagc tttgcttcag
     3661 accatggaag accaaggcaa ccagcgggtg caggcccatg cagcggctgc cctcattaac
     3721 ttcactgaag attgtcccaa gtcgctgctt attccctact tggataactt ggtgaagcac
     3781 ctgcactcca ttatggtgct caagctccag gagctgattc agaaaggcac caagttagtt
     3841 ttggaacaag ttgtgacatc cattgcatca gttgcagata ctgcagaaga aaagtttgtc
     3901 ccctactatg acttgtttat gccatcactg aagcacattg tcgagaacgc agtccagaag
     3961 gagctgagac tgctgcgagg gaagaccatc gagtgcatca gcctcatcgg gctggccgtc
     4021 gggaaggaga aattcatgca ggatgcttca gatgtgatgc agctattgtt gaagacacag
     4081 acagacttca atgatatgga agatgacgac ccccagattt cttacatgat ctcagcatgg
     4141 gccaggatgt gcaaaatcct tgggaaagaa ttccagcagt accttcccgt ggttatgggg
     4201 ccgctgatga agactgcttc aattaagcct gaagtggccc ttctagacac ccaggacatg
     4261 gagaatatga gtgatgatga cggttgggaa tttgtgaacc tcggagatca gcaaagtttt
     4321 ggtattaaaa ctgcaggact ggaagaaaag tcaacggctt gtcagatgtt ggtttgctat
     4381 gctaaggaat taaaggaagg ttttgtggaa tacactgaac aggttgtcaa actgatggtc
     4441 ccgctgctga aattttattt ccacgatggg gtccgagtgg cagcagcaga gtccatgcct
     4501 cttctgcttg agtgtgcgag agtccggggt cccgagtacc ttacacagat gtggcacttc
     4561 atgtgcgacg ctctcatcaa ggccatcggc acagagcccg actcagatgt cctctcggag
     4621 atcatgcatt cctttgcaaa gtgcattgag gtcatgggag acgggtgcct caacaatgag
     4681 cactttgagg agctgggagg catcctgaag gcgaagcttg aagaacattt caaaaatcag
     4741 gagttgcggc aagttaaaag acaagatgaa gactatgacg agcaggttga agagtcacta
     4801 caagatgagg atgataatga tgtttatata ctgactaaag tgtcggatat tttacactca
     4861 atattcagta gctacaaaga aaaggtgttg ccgtggtttg aacagctgct cccattaatt
     4921 gtcaacctga tttgtccgca aagaccatgg ccagacagac aatggggatt gtgcatcttc
     4981 gatgatatcg tagaacactg tagtccagct tcatttaagt atgcagaata tttcataagt
     5041 ccaatgctcc agtatgtgtg tgacaacagc ccagaagtca ggcaagctgc ggcatatggc
     5101 ctcggcgtca tggcgcagtt tggtggagat aactaccgcc ctttctgtac cgatgcactc
     5161 ccgctgcttg taagagtcat tcaggctcca gaggctaaga ccaaagaaaa tgtcaacgcc
     5221 acggagaact gcatctcagc agtgggaaag atcatgaagt tcaagcccga ctgtgtgaat
     5281 gtggaggagg tcttaccaca ctggctgtca tggctgcccc tacacgaaga taaagaagaa
     5341 gctgtccaga ccttcagtta cctgtgcgac ctgattgaaa gtaatcaccc aattgttctt
     5401 ggcccaaaca ataccaatct accgaaaata ttcagtataa ttgcagaagg agaaatgcat
     5461 gaggccatta aacatgaaga cccctgtgcc aaacgcctgg ccaatgtagt tcgccaagta
     5521 cagacttctg gaggattgtg gactgaatgc atagctcaac tcagtccgga gcagcaggca
     5581 gccatccagg agctcctgaa ctctgcctac ccatacgatg ttcctgacta tgcgggctat
     5641 ccgtatgacg tcccggacta tgcataggga tccctgcgat atccagcgcg gccgcgaagg
     5701 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5761 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5821 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5881 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5941 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6001 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6061 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6121 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6181 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6241 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     6301 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6361 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6421 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6481 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6541 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6601 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6661 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6721 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6781 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6841 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6901 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6961 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     7021 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     7081 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7141 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7201 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7261 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7321 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7381 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7441 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7501 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7561 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7621 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7681 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7741 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7801 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7861 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7921 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7981 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8041 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     8101 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8161 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag
     8221 acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     8281 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     8341 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     8401 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8461 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8521 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     8581 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     8641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8761 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8821 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8881 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     8941 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9001 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9061 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9121 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11101 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    11161 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    11221 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    11281 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    11341 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    11401 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    11461 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    11521 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
