LOCUS       V046413                10528 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046413
VERSION     V046413
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4623
                     /label="ITGB2(NM_000211)"
                     /note="ITGB2(NM_000211)"
                     /gene="ITGB2"
     promoter        4687..4898
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4911..5179
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5208..5217
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5238..5900
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5970..6023
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6024..6623
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6624..7211
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7285..7518
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7590..7711
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7730..7865
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7903..7919)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7927..7943
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7951..7981)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7996..8017
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8305..8893)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9064..9924)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9925..10029)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10503..10519
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tgggcctgcg ccccccactg
     2341 ctcgccctgg tggggctgct ctccctcggg tgcgtcctct ctcaggagtg cacgaagttc
     2401 aaggtcagca gctgccggga atgcatcgag tcggggcccg gctgcacctg gtgccagaag
     2461 ctgaacttca cagggccggg ggatcctgac tccattcgct gcgacacccg gccacagctg
     2521 ctcatgaggg gctgtgcggc tgacgacatc atggacccca caagcctcgc tgaaacccag
     2581 gaagaccaca atgggggcca gaagcagctg tccccacaaa aagtgacgct ttacctgcga
     2641 ccaggccagg cagcagcgtt caacgtgacc ttccggcggg ccaagggcta ccccatcgac
     2701 ctgtactatc tgatggacct ctcctactcc atgcttgatg acctcaggaa tgtcaagaag
     2761 ctaggtggcg acctgctccg ggccctcaac gagatcaccg agtccggccg cattggcttc
     2821 gggtccttcg tggacaagac cgtgctgccg ttcgtgaaca cgcaccctga taagctgcga
     2881 aacccatgcc ccaacaagga gaaagagtgc cagcccccgt ttgccttcag gcacgtgctg
     2941 aagctgacca acaactccaa ccagtttcag accgaggtcg ggaagcagct gatttccgga
     3001 aacctggatg cacccgaggg tgggctggac gccatgatgc aggtcgccgc ctgcccggag
     3061 gaaatcggct ggcgcaacgt cacgcggctg ctggtgtttg ccactgatga cggcttccat
     3121 ttcgcgggcg acgggaagct gggcgccatc ctgaccccca acgacggccg ctgtcacctg
     3181 gaggacaact tgtacaagag gagcaacgaa ttcgactacc catcggtggg ccagctggcg
     3241 cacaagctgg ctgaaaacaa catccagccc atcttcgcgg tgaccagtag gatggtgaag
     3301 acctacgaga aactcaccga gatcatcccc aagtcagccg tgggggagct gtctgaggac
     3361 tccagcaatg tggtccatct cattaagaat gcttacaata aactctcctc cagggtcttc
     3421 ctggatcaca acgccctccc cgacaccctg aaagtcacct acgactcctt ctgcagcaat
     3481 ggagtgacgc acaggaacca gcccagaggt gactgtgatg gcgtgcagat caatgtcccg
     3541 atcaccttcc aggtgaaggt cacggccaca gagtgcatcc aggagcagtc gtttgtcatc
     3601 cgggcgctgg gcttcacgga catagtgacc gtgcaggttc ttccccagtg tgagtgccgg
     3661 tgccgggacc agagcagaga ccgcagcctc tgccatggca agggcttctt ggagtgcggc
     3721 atctgcaggt gtgacactgg ctacattggg aaaaactgtg agtgccagac acagggccgg
     3781 agcagccagg agctggaagg aagctgccgg aaggacaaca actccatcat ctgctcaggg
     3841 ctgggggact gtgtctgcgg gcagtgcctg tgccacacca gcgacgtccc cggcaagctg
     3901 atatacgggc agtactgcga gtgtgacacc atcaactgtg agcgctacaa cggccaggtc
     3961 tgcggcggcc cggggagggg gctctgcttc tgcgggaagt gccgctgcca cccgggcttt
     4021 gagggctcag cgtgccagtg cgagaggacc actgagggct gcctgaaccc gcggcgtgtt
     4081 gagtgtagtg gtcgtggccg gtgccgctgc aacgtatgcg agtgccattc aggctaccag
     4141 ctgcctctgt gccaggagtg ccccggctgc ccctcaccct gtggcaagta catctcctgc
     4201 gccgagtgcc tgaagttcga aaagggcccc tttgggaaga actgcagcgc ggcgtgtccg
     4261 ggcctgcagc tgtcgaacaa ccccgtgaag ggcaggacct gcaaggagag ggactcagag
     4321 ggctgctggg tggcctacac gctggagcag caggacggga tggaccgcta cctcatctat
     4381 gtggatgaga gccgagagtg tgtggcaggc cccaacatcg ccgccatcgt cgggggcacc
     4441 gtggcaggca tcgtgctgat cggcattctc ctgctggtca tctggaaggc tctgatccac
     4501 ctgagcgacc tccgggagta caggcgcttt gagaaggaga agctcaagtc ccagtggaac
     4561 aatgataatc cccttttcaa gagcgccacc acgacggtca tgaaccccaa gtttgctgag
     4621 agttaggatc cctgcgatat ccagcgcggc cgcgaaggat ctgcgatcgc tccggtgccc
     4681 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     4741 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     4801 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     4861 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
     4921 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
     4981 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
     5041 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
     5101 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
     5161 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga
     5221 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     5281 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     5341 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     5401 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     5461 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     5521 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     5581 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     5641 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     5701 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     5761 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     5821 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     5881 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca
     5941 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg
     6001 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6061 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6121 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6181 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6241 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6301 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6361 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6421 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6481 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6541 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6601 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga
     6661 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6721 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6781 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6841 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6901 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6961 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7021 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7081 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7141 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc
     7201 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7261 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc
     7321 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7501 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7561 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7621 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7801 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7861 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt
     7921 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7981 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8101 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9361 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10021 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10081 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10141 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10201 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10261 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10321 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10381 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10441 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10501 ttgtaaaacg acggccagtg ccaagctg
//
