LOCUS       V029365                10622 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029365
VERSION     V029365
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2304..4697
                     /label="ITGB1(NM_002211)"
                     /note="ITGB1(NM_002211)"
                     /gene="ITGB1"
     CDS             4704..4769
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4820..5031
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5044..5312
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5347..6054
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6064..6117
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6118..6717
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6718..7305
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7379..7612
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7684..7805
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7824..7959
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7997..8013)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8021..8037
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8045..8075)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8090..8111
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8399..8987)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9158..10018)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10019..10123)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10597..10613
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattccgcc accatgaatt tacaaccaat tttctggatt ggactgatca
     2341 gttcagtttg ctgtgtgttt gctcaaacag atgaaaatag atgtttaaaa gcaaatgcca
     2401 aatcatgtgg agaatgtata caagcagggc caaattgtgg gtggtgcaca aattcaacat
     2461 ttttacagga aggaatgcct acttctgcac gatgtgatga tttagaagcc ttaaaaaaga
     2521 agggttgccc tccagatgac atagaaaatc ccagaggctc caaagatata aagaaaaata
     2581 aaaatgtaac caaccgtagc aaaggaacag cagagaagct caagccagag gatattactc
     2641 agatccaacc acagcagttg gttttgcgat taagatcagg ggagccacag acatttacat
     2701 taaaattcaa gagagctgaa gactatccca ttgacctcta ctaccttatg gacctgtctt
     2761 actcaatgaa agacgatttg gagaatgtaa aaagtcttgg aacagatctg atgaatgaaa
     2821 tgaggaggat tacttcggac ttcagaattg gatttggctc atttgtggaa aagactgtga
     2881 tgccttacat tagcacaaca ccagctaagc tcaggaaccc ttgcacaagt gaacagaact
     2941 gcaccagccc atttagctac aaaaatgtgc tcagtcttac taataaagga gaagtattta
     3001 atgaacttgt tggaaaacag cgcatatctg gaaatttgga ttctccagaa ggtggtttcg
     3061 atgccatcat gcaagttgca gtttgtggat cactgattgg ctggaggaat gttacacggc
     3121 tgctggtgtt ttccacagat gccgggtttc actttgctgg agatgggaaa cttggtggca
     3181 ttgttttacc aaatgatgga caatgtcacc tggaaaataa tatgtacaca atgagccatt
     3241 attatgatta tccttctatt gctcaccttg tccagaaact gagtgaaaat aatattcaga
     3301 caatttttgc agttactgaa gaatttcagc ctgtttacaa ggagctgaaa aacttgatcc
     3361 ctaagtcagc agtaggaaca ttatctgcaa attctagcaa tgtaattcag ttgatcattg
     3421 atgcatacaa ttccctttcc tcagaagtca ttttggaaaa cggcaaattg tcagaaggcg
     3481 taacaataag ttacaaatct tactgcaaga acggggtgaa tggaacaggg gaaaatggaa
     3541 gaaaatgttc caatatttcc attggagatg aggttcaatt tgaaattagc ataacttcaa
     3601 ataagtgtcc aaaaaaggat tctgacagct ttaaaattag gcctctgggc tttacggagg
     3661 aagtagaggt tattcttcag tacatctgtg aatgtgaatg ccaaagcgaa ggcatccctg
     3721 aaagtcccaa gtgtcatgaa ggaaatggga catttgagtg tggcgcgtgc aggtgcaatg
     3781 aagggcgtgt tggtagacat tgtgaatgca gcacagatga agttaacagt gaagacatgg
     3841 atgcttactg caggaaagaa aacagttcag aaatctgcag taacaatgga gagtgcgtct
     3901 gcggacagtg tgtttgtagg aagagggata atacaaatga aatttattct ggcaaattct
     3961 gcgagtgtga taatttcaac tgtgatagat ccaatggctt aatttgtgga ggaaatggtg
     4021 tttgcaagtg tcgtgtgtgt gagtgcaacc ccaactacac tggcagtgca tgtgactgtt
     4081 ctttggatac tagtacttgt gaagccagca acggacagat ctgcaatggc cggggcatct
     4141 gcgagtgtgg tgtctgtaag tgtacagatc cgaagtttca agggcaaacg tgtgagatgt
     4201 gtcagacctg ccttggtgtc tgtgctgagc ataaagaatg tgttcagtgc agagccttca
     4261 ataaaggaga aaagaaagac acatgcacac aggaatgttc ctattttaac attaccaagg
     4321 tagaaagtcg ggacaaatta ccccagccgg tccaacctga tcctgtgtcc cattgtaagg
     4381 agaaggatgt tgacgactgt tggttctatt ttacgtattc agtgaatggg aacaacgagg
     4441 tcatggttca tgttgtggag aatccagagt gtcccactgg tccagacatc attccaattg
     4501 tagctggtgt ggttgctgga attgttctta ttggccttgc attactgctg atatggaagc
     4561 ttttaatgat aattcatgac agaagggagt ttgctaaatt tgaaaaggag aaaatgaatg
     4621 ccaaatggga cacgggtgaa aatcctattt ataagagtgc cgtaacaact gtggtcaatc
     4681 cgaagtatga gggaaaactc gaggactaca aagaccatga cggtgattat aaagatcatg
     4741 acatcgacta caaggatgac gatgacaagt agggatccgc ggccgcgaag gatctgcgat
     4801 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4861 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4921 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4981 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5041 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5101 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5161 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     5221 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5281 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     5341 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     5401 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     5461 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     5521 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     5581 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     5641 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     5701 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     5761 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     5821 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     5881 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     5941 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     6001 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggatct
     6061 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6121 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6181 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6241 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6301 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6361 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6421 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6481 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6541 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6601 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6661 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     6721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7081 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc
     7261 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga
     7321 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7381 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     7441 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7501 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7561 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7621 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7681 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7801 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     7861 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7921 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     7981 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8821 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10141 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10201 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10381 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10441 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10501 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10561 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    10621 tg
//
