LOCUS       V035837                11713 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035837
VERSION     V035837
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5739
                     /label="ITGA7(NM_001144996)"
                     /note="ITGA7(NM_001144996)"
                     /gene="ITGA7"
     CDS             5746..5811
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5855..6066
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6079..6347
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6376..6385
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6406..7068
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7138..7191
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7192..7791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7792..8380
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8454..8687
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8759..8880
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8920..9055
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9088..9104)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9112..9128
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9136..9166)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9181..9202
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9490..10078)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10249..11109)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11110..11214)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11688..11704
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccggggctcg gagccgcgac
     2341 ccttgggggg cctccgggat ttgctacctt tttggctccc tgctcgtcga actgctcttc
     2401 tcacgggctg tcgccttcaa tctggacgtg atgggtgcct tgcgcaagga gggcgagcca
     2461 ggcagcctct tcggcttctc tgtggccctg caccggcagt tgcagccccg accccagagc
     2521 tggctgctgg tgggtgctcc ccaggccctg gctcttcctg ggcagcaggc gaatcgcact
     2581 ggaggcctct tcgcttgccc gttgagcctg gaggagactg actgctacag agtggacatc
     2641 gaccagggag ctgatatgca aaaggaaagc aaggagaacc agtggttggg agtcagtgtt
     2701 cggagccagg ggcctggggg caagattgtt acctgtgcac accgatatga ggcaaggcag
     2761 cgagtggacc agatcctgga gacgcgggat atgattggtc gctgctttgt gctcagccag
     2821 gacctggcca tccgggatga gttggatggt ggggaatgga agttctgtga gggacgcccc
     2881 caaggccatg aacaatttgg gttctgccag cagggcacag ctgccgcctt ctcccctgat
     2941 agccactacc tcctctttgg ggccccagga acctataatt ggaagggcac ggccagggtg
     3001 gagctctgtg cacagggctc agcggacctg gcacacctgg acgacggtcc ctacgaggcg
     3061 gggggagaga aggagcagga cccccgcctc atcccggtcc ctgccaacag ctactttggc
     3121 ttctctattg actcggggaa aggtctggtg cgtgcagaag agctgagctt tgtggctgga
     3181 gccccccgcg ccaaccacaa gggtgctgtg gtcatcctgc gcaaggacag cgccagtcgc
     3241 ctggtgcccg aggttatgct gtctggggag cgcctgacct ccggctttgg ctactcactg
     3301 gctgtggctg acctcaacag tgatggctgg ccagacctga tagtgggtgc cccctacttc
     3361 tttgagcgcc aagaagagct ggggggtgct gtgtatgtgt acttgaacca ggggggtcac
     3421 tgggctggga tctcccctct ccggctctgc ggctcccctg actccatgtt cgggatcagc
     3481 ctggctgtcc tgggggacct caaccaagat ggctttccag atattgcagt gggtgccccc
     3541 tttgatggtg atgggaaagt cttcatctac catgggagca gcctgggggt tgtcgccaaa
     3601 ccttcacagg tgctggaggg cgaggctgtg ggcatcaaga gcttcggcta ctccctgtca
     3661 ggcagcttgg atatggatgg gaaccaatac cctgacctgc tggtgggctc cctggctgac
     3721 accgcagtgc tcttcagggc cagacccatc ctccatgtct cccatgaggt ctctattgct
     3781 ccacgaagca tcgacctgga gcagcccaac tgtgctggcg gccactcggt ctgtgtggac
     3841 ctaagggtct gtttcagcta cattgcagtc cccagcagct atagccctac tgtggccctg
     3901 gactatgtgt tagatgcgga cacagaccgg aggctccggg gccaggttcc ccgtgtgacg
     3961 ttcctgagcc gtaacctgga agaacccaag caccaggcct cgggcaccgt gtggctgaag
     4021 caccagcatg accgagtctg tggagacgcc atgttccagc tccaggaaaa tgtcaaagac
     4081 aagcttcggg ccattgtagt gaccttgtcc tacagtctcc agacccctcg gctccggcga
     4141 caggctcctg gccaggggct gcctccagtg gcccccatcc tcaatgccca ccagcccagc
     4201 acccagcggg cagagatcca cttcctgaag caaggctgtg gtgaagacaa gatctgccag
     4261 agcaatctgc agctggtccg cgcccgcttc tgtacccggg tcagcgacac ggaattccaa
     4321 cctctgccca tggatgtgga tggaacaaca gccctgtttg cactgagtgg gcagccagtc
     4381 attggcctgg agctgatggt caccaacctg ccatcggacc cagcccagcc ccaggctgat
     4441 ggggatgatg cccatgaagc ccagctcctg gtcatgcttc ctgactcact gcactactca
     4501 ggggtccggg ccctggaccc tgcggagaag ccactctgcc tgtccaatga gaatgcctcc
     4561 catgttgagt gtgagctggg gaaccccatg aagagaggtg cccaggtcac cttctacctc
     4621 atccttagca cctccgggat cagcattgag accacggaac tggaggtaga gctgctgttg
     4681 gccacgatca gtgagcagga gctgcatcca gtctctgcac gagcccgtgt cttcattgag
     4741 ctgccactgt ccattgcagg aatggccatt ccccagcaac tcttcttctc tggtgtggtg
     4801 aggggcgaga gagccatgca gtctgagcgg gatgtgggca gcaaggtcaa gtatgaggtc
     4861 acggtttcca accaaggcca gtcgctcaga accctgggct ctgccttcct caacatcatg
     4921 tggcctcatg agattgccaa tgggaagtgg ttgctgtacc caatgcaggt tgagctggag
     4981 ggcgggcagg ggcctgggca gaaagggctt tgctctccca ggcccaacat cctccacctg
     5041 gatgtggaca gtagggatag gaggcggcgg gagctggagc cacctgagca gcaggagcct
     5101 ggtgagcggc aggagcccag catgtcctgg tggccagtgt cctctgctga gaagaagaaa
     5161 aacatcaccc tggactgcgc ccggggcacg gccaactgtg tggtgttcag ctgcccactc
     5221 tacagctttg accgcgcggc tgtgctgcat gtctggggcc gtctctggaa cagcaccttt
     5281 ctggaggagt actcagctgt gaagtccctg gaagtgattg tccgggccaa catcacagtg
     5341 aagtcctcca taaagaactt gatgctccga gatgcctcca cagtgatccc agtgatggta
     5401 tacttggacc ccatggctgt ggtggcagaa ggagtgccct ggtgggtcat cctcctggct
     5461 gtactggctg ggctgctggt gctagcactg ctggtgctgc tcctgtggaa gatgggattc
     5521 ttcaaacggg cgaagcaccc cgaggccacc gtgccccagt accatgcggt gaagattcct
     5581 cgggaagacc gacagcagtt caaggaggag aagacgggca ccatcctgag gaacaactgg
     5641 ggcagccccc ggcgggaggg cccggatgca caccccatcc tggctgctga cgggcatccc
     5701 gagctgggcc ccgatgggca tccagggcca ggcaccgccg gatccgacta caaagaccat
     5761 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     5821 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5881 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5941 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6001 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6061 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     6121 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     6181 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     6241 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     6301 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     6361 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     6421 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     6481 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     6541 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     6601 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     6661 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     6721 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     6781 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6841 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     6901 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     6961 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     7021 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     7081 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     7141 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     7201 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7261 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7321 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7381 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7441 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7501 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7561 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7621 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7681 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7741 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     7801 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7861 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7921 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7981 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8041 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8101 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8161 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8221 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8281 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8341 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     8401 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8461 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     8521 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8581 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8641 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8701 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8881 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     9061 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     9121 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9181 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9301 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9901 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11221 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    11281 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    11341 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    11401 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    11461 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11521 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11581 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11641 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11701 cagtgccaag ctg
//
