LOCUS       V032094                11237 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032094
VERSION     V032094
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6069
                     /label="IRS4(NM_001379150)"
                     /note="IRS4(NM_001379150)"
                     /gene="IRS4"
     CDS             6076..6102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6106..6132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6136..6162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6207..6418
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6431..6699
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6726..7325
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7332..7920
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7994..8227
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8299..8420
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8439..8574
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8612..8628)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8636..8652
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8660..8690)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8705..8726
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9014..9602)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9773..10633)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10634..10738)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11212..11228
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcgagt tgctccttca ctcgcgacca agcgacaaga
     2341 agactaagag gtgcagcagc ggcggcagcg gcagctctag cagcagtggt gaccaccccg
     2401 cttctttcct cgggaacccc gaccgcactc attgggaccg ggtcgtcttg tccgggagcc
     2461 atgtggctct ccacggccac tggctcccgg tcagactccg agtccgaaga ggaggacctg
     2521 cccgtcgggg aggaagtctg caaacgcggc tacctgcgga aacagaagca tgggcacagg
     2581 cgctacttcg tgctcaaact cgagactgct gacgccccag ctcggctgga atactacgaa
     2641 aatgccagga agttccggca cagtgtccgc gccgcggcgg ctgcagcagc ggcggccgcc
     2701 tctggcgccg cgatcccccc gctcattcca ccgcggcgcg tgatcaccct ataccagtgc
     2761 ttttccgtga gccagcgagc agatgcaagg taccgacacc tcattgctct tttcacccaa
     2821 gacgaatact tcgcgatggt ggccgagaac gagtcggagc aggaaagctg gtacttgctg
     2881 ctcagccgcc tcatcctcga gagcaagcgc cgccgctgcg gcacgctcgg tgcgcagccg
     2941 gacggagagc cggccgcgct ggcggcggca gcggcggcgg agccaccctt ctataaagat
     3001 gtgtggcagg taatagtcaa acccaggggg ctggggcaca gaaaagagct gagcggcgtg
     3061 ttccggctgt gtctaaccga cgaggaggtc gtgtttgtga ggctgaacac cgaagtggcc
     3121 agcgtggtcg tccagctcct gagcatccgt cgctgtggac actcggagca gtatttcttc
     3181 ttggaagtag gcaggtccac tgtcatcggt ccgggagagc tctggatgca ggtcgatgac
     3241 tgtgtggttg cccaaaacat gcatgagctg tttttggaga agatgagagc cttgtgtgca
     3301 gacgaataca gagcccgctg ccgcagctac agcatcagca tcggcgccca cctgttaacc
     3361 ctgctgtccg ctaggaggca cctgggcttg gtgccgctcg agccgggagg ctggctcaga
     3421 aggtcccgct ttgagcagtt ttgccacctc agggccatcg gcgacgggga agacgagatg
     3481 cttttcacca ggcgcttcgt aacacccagc gagcctgtgg cccactccag gcgaggaaga
     3541 ctgcacctgc ccagagggcg caggtcaagg agagcggttt cagtgccggc cagctttttt
     3601 cgccgcttag cacccagccc agcacgtccc cggcaccctg cagaagcccc gaacaatgga
     3661 gctcgcctgt cttctgaagt gtctggttct ggctctggca actttgggga ggaaggcaat
     3721 ccccagggca aagaagatca ggaaggaagc ggaggtgact acatgcctat gaacaattgg
     3781 ggctcaggaa atggccgggg ctcaggaggt ggccagggct caaatggcca aggctccagt
     3841 agccatagct cgggaggaaa ccagtgttca ggcgagggac agggatcccg aggtggtcag
     3901 ggctcaaatg gccagggctc aggaggaaac cagtgctcta gagatggcca gggcaccgca
     3961 ggtgggcacg gttcaggtgg tggccagaga cctggaggtg ggcatggctc tggtggtggt
     4021 caaggtccag gtgatggtca tggttctggt ggtggcaaaa acagcggtgg tggtaaaggc
     4081 tccggcagcg gtaaaggatc cgatggtgat ggtgaacgtg gaaaatctct gaagaaaaga
     4141 tcctattttg gcaaattaac tcaaagcaag caacagcaaa tgccaccacc tccaccacct
     4201 cctcctccac ccccaccagc tggaggaact ggtggaaaag ggaagtctgg gggaagattc
     4261 agactttatt tttgtgttga cagaggagcc acgaaagaat gcaaagaagc caaagaagtg
     4321 aaagatgcag agatcccaga aggtgcagct cgaggtcccc acagagccag agcttttgat
     4381 gaagatgagg atgacccata cgtgccaatg aggccagggg tggccacccc tcttgtaagc
     4441 tccagtgatt atatgccaat ggctcctcaa aatgtctctg cttcaaaaaa gcgccactct
     4501 cgatcccctt ttgaagattc aagagggtac atgatgatgt ttcccagagt gagcccacca
     4561 cctgctccga gtcctccaaa agcacctgat actaataaag aggatgactc aaaggacaat
     4621 gacagtgaga gtgactacat gtttatggct cctggagccg gtgcaattcc aaaaaacccc
     4681 agaaatcctc agggtggctc ttcctccaaa agttggagct cctacttctc tctaccaaac
     4741 ccttttcgga gctcaccttt gggacagaat gacaacagtg agtatgtgcc aatgttacct
     4801 ggaaagttcc tggggagggg cctagacaaa gaagtctcct ataactggga ccccaaagat
     4861 gcagcttcaa agccttcagg tgagggatca ttctcaaagc ctggagatgg gggatcacct
     4921 tcaaagcctt cagatcatga gcccccaaag aataaagcta agagacctaa ccgactttct
     4981 tttattacaa aaggatataa aatcaagcca aaaccacaaa agcccacaca tgagcagaga
     5041 gaagctgaca gctctagtga ctacgtcaac atggacttca ctaaaagaga gagcaataca
     5101 ccagctccct ctactcaagg actaccagat tcgtggggca taattgctga acccagacag
     5161 tcagcctttt ctaattatgt gaatgttgag tttggagtgc catttccaaa tccagcaaac
     5221 gacctctcag atcttttaag agctatacca cgtgccaacc ccttatctct ggacagtgct
     5281 aggtggccac ttcctcccct tcccctcagt gctacaggta gcaatgctat tgaggaagag
     5341 ggtgactaca ttgaagtaat tttcaactca gcaatgacac cagccatggc tcttgctgac
     5401 agtgccattc gctatgatgc tgaaacaggt cgaatctatg tggtcgaccc attttctgag
     5461 tgctgtatgg atatttctct ctcccccagc cgatgttctg aaccaccacc tgtagctagg
     5521 ctgctgcagg aagaagagca ggagagaaga cgcccacaaa gccgttctca aagtttcttt
     5581 gcagcagcca gagccgctgt ctctgctttt ccaacagaca gcctcgagag agacctttcc
     5641 ccatcctcag ccccggctgt cgcttcggct gcagagccga ctttagccct cagccaagtt
     5701 gtagctgcgg cctccgcgct cgccgcagcc ccgggcatcg gcgcagcagc cgcagctgct
     5761 ggatttgact ccgcctctgc ccgctggttt caacctgttg ctaatgctgc tgatgccgaa
     5821 gcagtaaggg gagcccaaga cgttgccggt ggctcgaacc ctggagccca caacccatct
     5881 gcaaaccttg ccagaggtga taaccaggct ggcggggctg ccgctgcagc tgccgctccg
     5941 gaaccaccac ctcgcagtcg ccgggtgcca agacccccgg agagagaaga ttctgacaac
     6001 gacgacgaca ctcacgtgag aatggatttt gccagacgtg ataatcagtt cgactctccc
     6061 aaaagagagg gatcctaccc atacgacgta cctgattatg caacttatcc ttatgatgta
     6121 cctgattatg ctagctatcc ttatgatgtc ccagactacg cataggcggc cgcgaaggat
     6181 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     6241 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     6301 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     6361 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     6421 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     6481 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     6541 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     6601 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     6661 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     6721 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     6781 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     6841 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6901 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6961 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     7021 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7081 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     7141 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7201 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7261 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     7321 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7381 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7441 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7501 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7561 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7621 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7681 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7741 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7801 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7861 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7921 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7981 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     8041 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8101 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8161 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8221 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8281 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8341 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8401 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8461 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8521 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     8581 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8641 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8701 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8761 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8821 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8881 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8941 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9001 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9061 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9181 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9421 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     9481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9661 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9721 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9841 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9901 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9961 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10021 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10081 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10141 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10201 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10261 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10321 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10381 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10621 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10741 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    10801 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    10861 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10921 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    10981 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    11041 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    11101 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    11161 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    11221 cggccagtgc caagctg
//
