LOCUS       V029683                12166 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029683
VERSION     V029683
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6192
                     /label="IFT122(NM_052985)"
                     /note="IFT122(NM_052985)"
                     /gene="IFT122"
     CDS             6199..6264
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6308..6519
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6532..6800
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6829..6838
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6859..7521
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7591..7644
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7645..8244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8245..8833
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8907..9140
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9212..9333
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9373..9508
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9541..9557)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9565..9581
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9589..9619)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9634..9655
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9943..10531)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10702..11562)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11563..11667)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12141..12157
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gggccgtgtt gacgtggaga
     2341 gataaagccg agcactgtat aaatgacatc gcatttaagc ctgatggaac tcaactgatt
     2401 ttggctgccg gaagcagatt actggtttat gacacctctg atggcacctt acttcagccc
     2461 ctcaagggac acaaagacac tgtgtactgt gtggcatatg cgaaggatgg caagcgcttt
     2521 gcttctggat cagctgacaa aagcgttatt atctggacat caaaactgga aggcattctg
     2581 aagtacacat cttggtctgt gatgtcttca ttgcacctcc atcttccatt tctgggcctc
     2641 cacaaaacag taagagtaac agccacagat aaagcaccta aaggccaagg tgggaggatt
     2701 gattgcttga ggccaagcgt tcaaaaccaa cctggccaaa agcacaatga tgctatacaa
     2761 tgtgtctcct acaatcctat tactcatcaa ctggcatctt gttcctccag tgactttggg
     2821 ttgtggtctc ctgaacagaa gtctgtctcc aaacacaaat caagcagcaa gatcatctgc
     2881 tgcagctgga caaatgatgg tcagtacctg gcgctgggga tgttcaatgg gatcatcagc
     2941 atacggaaca aaaatggcga ggagaaagta aagatcgagc ggccgggggg ctccctctcg
     3001 ccaatatggt ccatctgctg gaacccttca agccgatggg agagtttctg gatgaacaga
     3061 gagaatgagg atgccgagga tgtcattgtc aacagatata ttcaggaaat cccttccact
     3121 ctgaagtcag cagtgtacag tagtcagggt agtgaggcag aggaggaaga accagaggaa
     3181 gaggacgaca gtcccaggga cgacaactta gaggaacgta atgacatcct ggctgtggct
     3241 gactggggac agaaagtttc cttctaccag ctgagtggaa aacagattgg aaaggatcgg
     3301 gcactgaact ttgacccctg ctgcatcagc tactttacta aaggcgagta cattttgctg
     3361 gggggttcag acaagcaagt atctcttttc accaaggatg gagtgcggct tgggactgtt
     3421 ggggagcaga actcctgggt gtggacgtgt caagcgaaac cggattccaa ctatgtggtg
     3481 gtcggctgcc aggacggcac catttccttc taccagctta ttttcagcac agtccatggg
     3541 ctttacaagg accgctatgc ctacagggat agcatgactg acgtcattgt gcagcacctg
     3601 atcactgagc agaaagttcg gattaaatgc aaagagcttg tcaagaagat tgccatctac
     3661 agaaatcgat tggctatcca actgccagag aaaatcctca tctatgagtt gtattcagag
     3721 gacttatcag acatgcatta ccgggtaaag gagaagatta tcaagaagtt tgagtgcaac
     3781 ctcctggtgg tgtgtgccaa tcacatcatc ctgtgccagg agaaacggct gcagtgcctg
     3841 tccttcagcg gagtgaagga gcgggagtgg cagatggagt ctctcattcg ttacatcaag
     3901 gtgatcggtg gccctcctgg aagagaaggc ctcttagtgg ggctgaagaa tggacagatc
     3961 ctgaagatct tcgtggacaa tctctttgct atcgtcctgc tgaagcaggc cacagctgtg
     4021 cgctgcttgg acatgagtgc ctcccgtaag aagctggccg tggtagatga aaatgacact
     4081 tgcctggtgt atgacatcga caccaaggag ctgctttttc aggaaccaaa cgccaacagt
     4141 gtagcttgga acacccagtg tgaggacatg ctctgcttct cgggaggagg ctacctcaac
     4201 atcaaagcca gcaccttccc tgtgcaccgg cagaagctgc agggctttgt ggtcggctac
     4261 aatggctcca agatcttctg cctccatgtc ttctccattt ctgccgtgga ggtgccgcag
     4321 tccgctccca tgtaccagta cctggatagg aaactgttca aggaagccta ccagattgct
     4381 tgcttgggtg tcacagacac tgattggcgt gaactggcca tggaagcgct agaaggttta
     4441 gattttgaaa cagcaaagaa ggccttcatc agagtacaag acctccgata tttagagctc
     4501 atcagcagca ttgaggagag gaagaagcgg ggagagacca acaatgacct gtttctggca
     4561 gatgtgtttt cctaccaggg gaagttccat gaggccgcca aactgtacaa gaggagtggg
     4621 cacgagaacc tcgcgcttga aatgtacacc gacctctgca tgtttgagta tgccaaggat
     4681 ttccttggat ctggagaccc caaagaaaca aagatgctaa tcaccaaaca ggctgactgg
     4741 gccagaaata tcaaggagcc caaagccgcc gtggagatgt acatctcagc aggagagcac
     4801 gtcaaggcca tcgagatctg tggtgaccat ggctgggttg acatgttgat cgacatcgcc
     4861 cgcaaactgg acaaggctga gcgcgagccc ctgctgctgt gcgctaccta cctcaagaag
     4921 ctggacagcc ctggctatgc tgctgagacc tacctgaaga tgggtgacct caagtccctg
     4981 gtgcagctgc acgtggagac ccagcgctgg gatgaggcct ttgctttggg tgagaagcat
     5041 cctgagttta aggatgacat ctacatgccg tatgctcagt ggctagcaga gaacgatcgc
     5101 tttgaggaag cccagaaagc gttccacaag gctgggcgac agagagaagc ggtccaggtg
     5161 ctggagcagc tcacaaacaa tgccgtggcg gagagcaggt ttaatgatgc tgcctattat
     5221 tactggatgc tgtccatgca gtgcctcgat atagctcaag atcctgccca gaaggacaca
     5281 atgcttggca agttctacca cttccagcgt ttggcagagc tgtaccatgg ttaccatgcc
     5341 atccatcgcc acacggaaga tccgttcagt gtccatcgtc ctgaaactct tttcaacatc
     5401 tccaggttcc tgctgcacag cctgcccaag gacaccccct cgggcatctc taaagtgaaa
     5461 atactcttca ccttggccaa gcagagcaag gccctcggtg cctacaggct ggcccggcac
     5521 gcctatgaca agctgcgtgg cctgtacatc cctgccagat tccaaaagtc cattgagctg
     5581 ggtaccctga ccatccgcgc caagcccttc cacgacagtg aggagttggt gcccttgtgc
     5641 taccgctgct ccaccaacaa cccgctgctc aacaacctgg gcaacgtctg catcaactgc
     5701 cgccagccct tcatcttctc cgcctcttcc tacgacgtgc tacacctggt tgagttctac
     5761 ctggaggaag ggatcactga tgaagaagcc atctccctca tcgacctgga ggtgctgaga
     5821 cccaagcggg atgacagaca gctagagatt gcaaacaaca gctcccagat tctgcggcta
     5881 gtggagacca aggactccat cggagatgag gacccgttca cagctaagct gagctttgag
     5941 caaggtggct cagagttcgt gccagtggtg gtgagccggc tggtgctgcg ctccatgagc
     6001 cgccgggatg tcctcatcaa gcgatggccc ccacccctga ggtggcaata cttccgctca
     6061 ctgctgcctg acgcctccat taccatgtgc ccctcctgct tccagatgtt ccattctgag
     6121 gactatgagt tgctggtgct tcagcatggc tgctgcccct actgccgcag gtgcaaggat
     6181 gaccctggcc caggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     6241 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     6301 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6361 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6421 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6481 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     6541 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6601 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6661 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6721 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6781 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6841 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6901 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6961 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     7021 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     7081 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     7141 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     7201 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     7261 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     7321 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     7381 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7441 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7501 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     7561 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7621 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     8041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8221 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     8281 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8341 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8401 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8461 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8521 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8581 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8641 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8701 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8761 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8821 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8881 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8941 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9001 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9061 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9121 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9181 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9241 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9301 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     9361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9481 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     9541 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9601 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9661 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9721 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10381 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10681 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10741 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10801 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10861 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10921 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10981 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11041 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11101 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11161 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11221 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11281 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11341 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11401 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11461 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11521 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11581 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11641 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11701 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11761 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11821 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11881 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11941 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    12001 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    12061 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    12121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
