LOCUS       V041768                11309 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041768
VERSION     V041768
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2296..2988
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             3025..5736
                     /label="TLR3(NM_003265)"
                     /note="TLR3(NM_003265)"
                     /gene="TLR3"
     CDS             5743..5808
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5852..6063
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6076..6344
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6372..7397
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    7404..7992
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8066..8299
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8371..8492
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8511..8646
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8684..8700)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8708..8724
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8732..8762)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8777..8798
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9086..9674)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9845..10705)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10706..10810)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11284..11300
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg ccaccatgag cgagctgatc aaggagaaca tgcacatgaa gctgtacatg
     2341 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     2401 gagggcaccc agaccatgaa gatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     2461 gacatcctgg ctaccagctt catgtacggc agcaaagcct tcatcaacca cacccagggc
     2521 atccccgact tctttaagca gtccttccct gagggcttca catgggagag aatcaccaca
     2581 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcttccagaa cggctgcatc
     2641 atctacaacg tcaagatcaa cggggtgaac ttcccatcca acggccctgt gatgcagaag
     2701 aaaacacgcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctgaga
     2761 ggccacagcc agatggccct gaagctcgtg ggcgggggct acctgcactg ctccttcaag
     2821 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggctt ccacttcgtg
     2881 gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     2941 atggctgtgg ccaagtactg cgacctccct agcaaactgg ggcacagagg atccggtgga
     3001 ggcggatcag gaggcggtgg atctatgaga cagactttgc cttgtatcta cttttggggg
     3061 ggccttttgc cctttgggat gctgtgtgca tcctccacca ccaagtgcac tgttagccat
     3121 gaagttgctg actgcagcca cctgaagttg actcaggtac ccgatgatct acccacaaac
     3181 ataacagtgt tgaaccttac ccataatcaa ctcagaagat taccagccgc caacttcaca
     3241 aggtatagcc agctaactag cttggatgta ggatttaaca ccatctcaaa actggagcca
     3301 gaattgtgcc agaaacttcc catgttaaaa gttttgaacc tccagcacaa tgagctatct
     3361 caactttctg ataaaacctt tgccttctgc acgaatttga ctgaactcca tctcatgtcc
     3421 aactcaatcc agaaaattaa aaataatccc tttgtcaagc agaagaattt aatcacatta
     3481 gatctgtctc ataatggctt gtcatctaca aaattaggaa ctcaggttca gctggaaaat
     3541 ctccaagagc ttctattatc aaacaataaa attcaagcgc taaaaagtga agaactggat
     3601 atctttgcca attcatcttt aaaaaaatta gagttgtcat cgaatcaaat taaagagttt
     3661 tctccagggt gttttcacgc aattggaaga ttatttggcc tctttctgaa caatgtccag
     3721 ctgggtccca gccttacaga gaagctatgt ttggaattag caaacacaag cattcggaat
     3781 ctgtctctga gtaacagcca gctgtccacc accagcaata caactttctt gggactaaag
     3841 tggacaaatc tcactatgct cgatctttcc tacaacaact taaatgtggt tggtaacgat
     3901 tcctttgctt ggcttccaca actagaatat ttcttcctag agtataataa tatacagcat
     3961 ttgttttctc actctttgca cgggcttttc aatgtgaggt acctgaattt gaaacggtct
     4021 tttactaaac aaagtatttc ccttgcctca ctccccaaga ttgatgattt ttcttttcag
     4081 tggctaaaat gtttggagca ccttaacatg gaagataatg atattccagg cataaaaagc
     4141 aatatgttca caggattgat aaacctgaaa tacttaagtc tatccaactc ctttacaagt
     4201 ttgcgaactt tgacaaatga aacatttgta tcacttgctc attctccctt acacatactc
     4261 aacctaacca agaataaaat ctcaaaaata gagagtgatg ctttctcttg gttgggccac
     4321 ctagaagtac ttgacctggg ccttaatgaa attgggcaag aactcacagg ccaggaatgg
     4381 agaggtctag aaaatatttt cgaaatctat ctttcctaca acaagtacct gcagctgact
     4441 aggaactcct ttgccttggt cccaagcctt caacgactga tgctccgaag ggtggccctt
     4501 aaaaatgtgg atagctctcc ttcaccattc cagcctcttc gtaacttgac cattctggat
     4561 ctaagcaaca acaacatagc caacataaat gatgacatgt tggagggtct tgagaaacta
     4621 gaaattctcg atttgcagca taacaactta gcacggctct ggaaacacgc aaaccctggt
     4681 ggtcccattt atttcctaaa gggtctgtct cacctccaca tccttaactt ggagtccaac
     4741 ggctttgacg agatcccagt tgaggtcttc aaggatttat ttgaactaaa gatcatcgat
     4801 ttaggattga ataatttaaa cacacttcca gcatctgtct ttaataatca ggtgtctcta
     4861 aagtcattga accttcagaa gaatctcata acatccgttg agaagaaggt tttcgggcca
     4921 gctttcagga acctgactga gttagatatg cgctttaatc cctttgattg cacgtgtgaa
     4981 agtattgcct ggtttgttaa ttggattaac gagacccata ccaacatccc tgagctgtca
     5041 agccactacc tttgcaacac tccacctcac tatcatgggt tcccagtgag actttttgat
     5101 acatcatctt gcaaagacag tgcccccttt gaactctttt tcatgatcaa taccagtatc
     5161 ctgttgattt ttatctttat tgtacttctc atccactttg agggctggag gatatctttt
     5221 tattggaatg tttcagtaca tcgagttctt ggtttcaaag aaatagacag acagacagaa
     5281 cagtttgaat atgcagcata tataattcat gcctataaag ataaggattg ggtctgggaa
     5341 catttctctt caatggaaaa ggaagaccaa tctctcaaat tttgtctgga agaaagggac
     5401 tttgaggcgg gtgtttttga actagaagca attgttaaca gcatcaaaag aagcagaaaa
     5461 attatttttg ttataacaca ccatctatta aaagacccat tatgcaaaag attcaaggta
     5521 catcatgcag ttcaacaagc tattgaacaa aatctggatt ccattatatt ggttttcctt
     5581 gaggagattc cagattataa actgaaccat gcactctgtt tgcgaagagg aatgtttaaa
     5641 tctcactgca tcttgaactg gccagttcag aaagaacgga taggtgcctt tcgtcataaa
     5701 ttgcaagtag cacttggatc caaaaactct gtacatctcg aggactacaa agaccatgac
     5761 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta ggcggccgca
     5821 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5881 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5941 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     6001 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     6061 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     6121 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     6181 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     6241 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     6301 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     6361 cctacgtcga catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     6421 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     6481 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     6541 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     6601 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     6661 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     6721 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     6781 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     6841 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     6901 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     6961 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     7021 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     7081 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     7141 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     7201 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     7261 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     7321 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     7381 cgagggcaaa ggaataggtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     7441 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7501 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7561 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7621 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7681 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7741 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7801 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7861 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7921 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7981 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8041 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
     8101 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8161 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8221 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8281 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8341 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8521 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8581 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8641 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg
     8701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9001 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9061 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10801 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    10861 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    10921 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    10981 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    11041 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    11101 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    11161 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    11221 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    11281 gttgtaaaac gacggccagt gccaagctg
//
