LOCUS       V028294                10718 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028294
VERSION     V028294
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2290..3003
                     /codon_start=1
                     /note="excitable with blue light"
                     /product="S65T variant of Aequorea victoria green
                     fluorescent protein (Heim et al., 1995)"
                     /transl_table=1
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
                     /label="GFP (S65T)"
     CDS             3040..5211
                     /label="HSP90AB1(NM_001271969)"
                     /note="HSP90AB1(NM_001271969)"
                     /gene="HSP90AB1"
     promoter        5261..5472
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5485..5753
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5781..6806
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    6813..7401
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7475..7708
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7780..7901
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7920..8055
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8093..8109)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8117..8133
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8141..8171)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8186..8207
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8495..9083)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9254..10114)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10115..10219)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10693..10709
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagccacca tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     2341 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     2401 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     2461 ccaacacttg tcactacttt cacttatggt gttcaatgct tttcaagata cccagatcat
     2521 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     2581 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     2641 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     2701 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     2761 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     2821 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     2881 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     2941 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     3001 aaaggcggtg gaggcagtgg tggaggcggt agcctcgaga tgcctgagga agtgcaccat
     3061 ggagaggagg aggtggagac ttttgccttt caggcagaaa ttgcccaact catgtccctc
     3121 atcatcaata ccttctattc caacaaggag attttccttc gggagttgat ctctaatgct
     3181 tctgatgcct tggacaagat tcgctatgag agcctgacag acccttcgaa gttggacagt
     3241 ggtaaagagc tgaaaattga catcatcccc aaccctcagg aacgtaccct gactttggta
     3301 gacacaggca ttggcatgac caaagctgat ctcataaata atttgggaac cattgccaag
     3361 tctggtacta aagcattcat ggaggctctt caggctggtg cagacatctc catgattggg
     3421 cagtttggtg ttggctttta ttctgcctac ttggtggcag agaaagtggt tgtgatcaca
     3481 aagcacaacg atgatgaaca gtatgcttgg gagtcttctg ctggaggttc cttcactgtg
     3541 cgtgctgacc atggtgagcc cattggcagg ggtaccaaag tgatcctcca tcttaaagaa
     3601 gatcagacag agtacctaga agagaggcgg gtcaaagaag tagtgaagaa gcattctcag
     3661 ttcataggct atcccatcac cctttatttg gagaaggaac gagagaagga aattagtgat
     3721 gatgaggcag aggaagagaa aggtgagaaa gaagaggaag ataaagatga tgaagaaaaa
     3781 cccaagatcg aagatgtggg ttcagatgag gaggatgaca gcggtaagga taagaagaag
     3841 aaaactaaga agatcaaaga gaaatacatt gatcaggaag aactaaacaa gaccaagcct
     3901 atttggacca gaaaccctga tgacatcacc caagaggagt atggagaatt ctacaagagc
     3961 ctcactaatg actgggaaga ccacttggca gtcaagcact tttctgtaga aggtcagttg
     4021 gaattcaggg cattgctatt tattcctcgt cgggctccct ttgacctttt tgagaacaag
     4081 aagaaaaaga acaacatcaa actctatgtc cgccgtgtgt tcatcatgga cagctgtgat
     4141 gagttgatac cagagtatct caattttatc cgtggtgtgg ttgactctga ggatctgccc
     4201 ctgaacatct cccgagaaat gctccagcag agcaaaatct tgaaagtcat tcgcaaaaac
     4261 attgttaaga agtgccttga gctcttctct gagctggcag aagacaagga gaattacaag
     4321 aaattctatg aggcattctc taaaaatctc aagcttggaa tccacgaaga ctccactaac
     4381 cgccgccgcc tgtctgagct gctgcgctat catacctccc agtctggaga tgagatgaca
     4441 tctctgtcag agtatgtttc tcgcatgaag gagacacaga agtccatcta ttacatcact
     4501 ggtgagagca aagagcaggt ggccaactca gcttttgtgg agcgagtgcg gaaacggggc
     4561 ttcgaggtgg tatatatgac cgagcccatt gacgagtact gtgtgcagca gctcaaggaa
     4621 tttgatggga agagcctggt ctcagttacc aaggagggtc tggagctgcc tgaggatgag
     4681 gaggagaaga agaagatgga agagagcaag gcaaagtttg agaacctctg caagctcatg
     4741 aaagaaatct tagataagaa ggttgagaag gtgacaatct ccaatagact tgtgtcttca
     4801 ccttgctgca ttgtgaccag cacctacggc tggacagcca atatggagcg gatcatgaaa
     4861 gcccaggcac ttcgggacaa ctccaccatg ggctatatga tggccaaaaa gcacctggag
     4921 atcaaccctg accaccccat tgtggagacg ctgcggcaga aggctgaggc cgacaagaat
     4981 gataaggcag ttaaggacct ggtggtgctg ctgtttgaaa ccgccctgct atcttctggc
     5041 ttttcccttg aggatcccca gacccactcc aaccgcatct atcgcatgat caagctaggt
     5101 ctaggtattg atgaagatga agtggcagca gaggaaccca atgctgcagt tcctgatgag
     5161 atcccccctc tcgagggcga tgaggatgcg tctcgcatgg aagaagtcga ttagggatcc
     5221 gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5281 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5341 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5401 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5461 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5521 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5581 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5641 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5701 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5761 tgaccggcgc ctacgtcgac atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5821 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5881 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5941 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     6001 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     6061 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     6121 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     6181 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     6241 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     6301 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     6361 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6421 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     6481 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6541 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6601 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6661 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6721 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6781 gcactcgtcc gagggcaaag gaataggtcg acaatcaacc tctggattac aaaatttgtg
     6841 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6901 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6961 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7021 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7081 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7141 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7201 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7261 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7321 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7381 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     7441 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7501 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7561 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7621 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7681 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7741 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7801 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7861 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8041 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc
     8101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9001 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9061 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9121 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9181 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9241 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9301 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9361 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9421 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9481 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9541 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9601 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9661 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9721 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9781 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9841 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9901 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9961 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10021 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10081 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10141 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10201 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10261 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10321 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10381 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10441 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10501 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
    10561 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10621 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10681 agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
