LOCUS       V047564                10832 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047564
VERSION     V047564
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5260
                     /label="FHIP1B(NM_032127)"
                     /note="FHIP1B(NM_032127)"
                     /gene="FHIP1B"
     CDS             5267..5332
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5375..5586
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5599..5867
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5895..6920
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    6927..7515
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7589..7822
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7894..8015
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8034..8169
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8207..8223)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8231..8247
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8255..8285)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8300..8321
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8609..9197)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9368..10228)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10229..10333)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10807..10823
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc ggatccgcca ccatggagag gatgaattgg ctgagcagac tggcctcccg
     2341 gggccctggg caccgtatac ctcaaggggc caatctccaa accccagtca tggctgatcc
     2401 cgagacctgc ctcatggtct tcaagaatca ctggtcccag gtggtgcgaa tcctggagcg
     2461 gcaaggccct cgggcagctc ctgggggtgc agacgatctc agtgctgtgc gcaaccacac
     2521 ttaccagatg ttgacactgc tggcagagga ccgtgcagtt ccctcggccc ccacaggccc
     2581 tgggcccctg ctggagtttg ctctgcacga ggatctgctg acccgtgtgt tgacatggca
     2641 gctgcaatgg gatgagcttg gggatggggt cgaggaacgg cgggctgagc aactgaaact
     2701 atttgaaatg ctagtgagcg aagctcgcca gccactgttg cggcatggtc cagttcgtga
     2761 ggctctgctc accctgctgg atgcctgtgg ccgccctgtg cccagtagcc cagcactgga
     2821 tgaaggcttg gtgctacttc tcagccagct gtgtgtttgt gtggcccagg agccttcatt
     2881 gctcgagttc ttcctgcagc cacctcctga gcctggagcc gctccccgtc ttcttctctt
     2941 ttctcgcctt gtcccttttg tgcatcgaga gggcaccctg ggccagcagg cccgtgatgc
     3001 cctacttctt ctcatggctt tgtcagctgg gagccccact gtgggccgct acatcgcgga
     3061 tcactcttac ttctgcccgg tgctggccac agggctcagt gccctgtact catcactgcc
     3121 tcgaaagatt gaggttccag gggatgattg gcactgtctg cgacgggaag actggctggg
     3181 agtgccagcc cttgcactct tcatgagttc cctggagttc tgcaatgcag taattcaggt
     3241 ggctcacccc ctggtgcaga agcagttggt tgattatatc cataatgggt tcctggtgcc
     3301 tgtcatgggt cctgccttgc acaagacctc tgtggaggag atgatcgcca gtaccgccta
     3361 tctggaactt ttcctacgga gtatctcaga gcctgctttg ctccgtacct tcctgcgatt
     3421 cctgttgttg caccggcatg acacccacac catcctcgac accctcgttg ctcgtattgg
     3481 cagtaactcc cggctctgca tggtctctct gagtctcttc aggaccctcc tgaacctcag
     3541 ctgtgaggat gtcctgctgc agctggttct caggtatctt gttccatgta accacgttat
     3601 gctgagccag aagccggctg ttcgtgatgt ggacctatat ggacgagcag ctgacaagtt
     3661 tctctcccta atcccacgct gttgtcggca ccacgccccc agcccacctc gtccagagca
     3721 tgcctcatgg gcacgaggtg ggcctagcag agagacaggg agaagggagg acatcacggg
     3781 tcctggaagc ccaagtgtgg actcctcttc tgtgacgaca gtaccccggc cctccacacc
     3841 atctcgtctg gctctcttcc tgcggcagca gagcctgggt ggctctgagt ctccaggccc
     3901 agccccttgc tcaccagggc tttctgcatc ccccgcctcc agccctggcc gacggcctac
     3961 ccctgcagag gagcctggag agctggaaga caattacctg gagtatctgc gtgaggcacg
     4021 tcgtggtgtg gaccgctgtg tccgagcctg ccgtacctgg tctgccccct atgatggcga
     4081 gcggccctct cctgagccca gtccttttgg ctcccggact aagaaacgca gcctactgcc
     4141 tgaggaggac aggaacaacg tgggggaagg ggaggaggaa gagctgggga ggagggggcg
     4201 ggctgggggt gcaggggagg gccctggtca cctgccccct ccccagctca atggagtgcc
     4261 aggatcatgg cctgaggggg ccaagaaggt tcgtctggtg ccaaaggagg gagctgggga
     4321 actgctagag ggcatctccg agggcatggc aggactagag ggctttgggc aggagctccg
     4381 ggagctagag gtggcattga gcaatggggg aactggctca gagtccccct tagaacctcc
     4441 actgcccctt gaggaggagg aggcctacga gagcttcacc tgtccccctg agccccctgg
     4501 ccccttcctc agcagccctt tgcggactct caaccagctg ccaagccagc ccttcactgg
     4561 ccccttcatg gctgtgctct ttgccaaact cgagaacatg ctgcagaact ccgtctatgt
     4621 caacttcctg ctgacggggc tggtggccca gctggcctgt cacccccagc ccctgctccg
     4681 ctctttcctg ctcaacacca acatggtctt ccagcccagt gtcaagtccc tgctgcaggt
     4741 gctgggctct gtgaagaata agattgagaa ctttgcggct tcccaggagg acttcccagc
     4801 actgctgtcc aaagccaaga agtacctcat tgcccgtggc aagttggact gggctgaggg
     4861 ccctgcagca ggacctgccc cacgccgttc tgatccccta gtgaagagcc ggaggccatc
     4921 cttgggggag ttactcctgc ggcatgcaca cagtccaacc agggcccggc aggcggcaca
     4981 attggtcctt cagcctgggc gagacggagc aggacttggc ctaagtgggg gctcccctgg
     5041 ggcttcaact ccagttctac tcacccgggg cggggcccct gaacgccaag gtgaggctct
     5101 tcgagtcaag aatgctgtct actgtgcagt cattttccct gaatttctca aggagttggc
     5161 tgccatctcc caggctcatg ccgtcacctc gcctttcttg ttggagactt cagaggaagg
     5221 atctggccct ctcatctcag gctgtgggcc cctcaatcct ctcgaggact acaaagacca
     5281 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc
     5341 gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5401 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5461 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5521 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5581 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5641 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5701 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5761 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5821 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5881 gcgcctacgt cgacatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     5941 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     6001 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     6061 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     6121 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     6181 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     6241 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     6301 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     6361 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     6421 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     6481 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     6541 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     6601 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     6661 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     6721 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     6781 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     6841 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     6901 gtccgagggc aaaggaatag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     6961 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7021 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7081 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7141 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7201 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7261 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7321 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7381 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7441 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7501 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7561 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     7621 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7861 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8041 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8101 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8161 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     8221 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8281 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8341 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8401 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8581 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8641 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8701 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8761 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8821 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8881 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8941 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9001 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9061 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10321 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10381 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    10441 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    10501 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    10561 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    10621 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    10681 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    10741 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    10801 gacgttgtaa aacgacggcc agtgccaagc tg
//
