LOCUS       V047130                10286 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047130
VERSION     V047130
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4714
                     /label="EXOC6(NM_019053)"
                     /note="EXOC6(NM_019053)"
                     /gene="EXOC6"
     CDS             4721..4786
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4829..5040
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5053..5321
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5349..6374
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    6381..6969
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7043..7276
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7348..7469
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7488..7623
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7661..7677)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7685..7701
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7709..7739)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7754..7775
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8063..8651)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8822..9682)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9683..9787)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10261..10277
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc ggatccgcca ccatggcgga gaacagcgag agtctgggca ccgtccccga
     2341 gcacgagcgg atcttgcagg agatcgagag caccgacacc gcctgtgtgg ggcccaccct
     2401 ccggtctgtg tatgatgacc aaccaaatgc gcacaagaag tttatggaaa agttagatgc
     2461 ttgtatccgt aatcatgaca aggaaattga aaagatgtgt aattttcatc atcagggttt
     2521 tgtagatgct attacagaac tccttaaagt aaggactgat gcagaaaaac tgaaggtgca
     2581 agttactgat accaaccgaa ggtttcaaga tgctggaaaa gaggtgatag tccacacaga
     2641 agatatcatt cgatgtagaa ttcagcagag aaatattaca actgtagtag aaaaattgca
     2701 gttatgcctt cctgtgctag aaatgtacag taagctgaaa gaacagatga gtgccaaaag
     2761 gtactattct gccctaaaaa ctatggaaca attagagaat gtgtactttc cctgggttag
     2821 tcaataccgg ttttgtcagc tcatgataga aaatcttccc aaactccgtg aggatattaa
     2881 agaaatctcc atgtctgatc tcaaagactt tttggaaagt attcgaaaac attctgacaa
     2941 aataggtgaa acagcaatga aacaggcaca gcatcagaaa accttcagtg tttctctgca
     3001 gaaacaaaat aaaatgaaat ttgggaaaaa tatgtatata aatcgtgata gaattccaga
     3061 ggaaaggaat gaaactgtat tgaaacattc acttgaagaa gaggatgaga atgaagaaga
     3121 gatcttaact gttcaggatc ttgttgattt ttcccctgtt tatcgatgtt tgcacattta
     3181 ttctgttttg ggtgacgagg aaacatttga aaactattat cgaaaacaaa gaaagaaaca
     3241 agcaagactg gtattgcaac cccagtcgaa tatgcatgaa acagttgatg gctatagaag
     3301 atatttcact caaattgtag ggttctttgt ggtagaagat cacattttac atgtgaccca
     3361 aggattagta accagggcat acactgatga actttggaac atggccctct caaagataat
     3421 tgctgtcctt agagctcatt catcctattg cactgatcct gatcttgttc tggagctgaa
     3481 gaatcttact gtaatatttg cagatacttt acagggttat ggttttccag tgaaccgact
     3541 ttttgacctt ttatttgaaa taagagacca atacaatgaa acactgctta agaaatgggc
     3601 tggagttttc agggacattt ttgaagaaga taattacagc cccatccctg ttgtcaatga
     3661 agaagaatat aaaattgtca tcagcaaatt tccctttcaa gatccagacc ttgaaaagca
     3721 gtctttccca aagaaattcc ccatgtctca gtcagtgcct catatttaca ttcaagttaa
     3781 agaatttatt tatgccagcc ttaaattttc agagtcacta caccggagct caacagaaat
     3841 agacgatatg cttagaaaat caacaaatct gctgctgacc agaactttga gtagctgttt
     3901 actgaacctt attagaaaac ctcatatagg tttgacagag ctggtacaaa tcatcataaa
     3961 cacaacacac ctggagcaag cttgtaaata tcttgaggac tttataacta acattacaaa
     4021 tatttcccaa gaaactgttc atactacaag actttatgga ctttctactt tcaaggatgc
     4081 tcgacatgca gcagaaggag aaatatatac caaactgaat caaaaaattg atgaatttgt
     4141 tcagcttgct gattatgact ggacaatgtc tgagccagat ggaagagcta gtggttattt
     4201 aatggacctt ataaattttt tgagaagcat ctttcaagtg tttactcatt tgcctgggaa
     4261 agttgctcag acagcttgca tgtcagcctg ccagcatctg tcaacatcct taatgcagat
     4321 gctactggac agtgagttaa aacaaataag catgggagct gttcagcagt ttaacttaga
     4381 tgtcatacag tgtgaattgt ttgccagctc tgagcctgtg ccaggattcc agggggatac
     4441 cctgcagcta gcattcattg acctcagaca actccttgac ctgtttatgg tttgggattg
     4501 gtctacttac ctagctgatt atgggcagcc agcttctaag taccttcggg tgaatccaaa
     4561 cacagccctt actcttttgg agaagatgaa ggatactagc aaaaagaaca atatatttgc
     4621 tcagttcagg aagaatgatc gagacaaaca gaagttgata gagacagtcg tgaaacagct
     4681 gagaagtttg gtgaatggta tgtcccagca catgctcgag gactacaaag accatgacgg
     4741 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcaagg
     4801 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4861 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4921 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4981 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5041 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5101 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5161 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5221 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5281 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5341 acgtcgacat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     5401 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     5461 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     5521 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     5581 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     5641 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     5701 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     5761 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     5821 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     5881 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     5941 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     6001 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     6061 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     6121 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     6181 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     6241 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     6301 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     6361 gggcaaagga ataggtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6421 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6481 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6541 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6601 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6661 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6721 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6781 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6841 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6901 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6961 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7021 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     7081 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7141 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7201 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7261 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7321 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7441 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7501 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7561 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7621 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     7681 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7741 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7801 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7861 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7921 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7981 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8041 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8101 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8161 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8221 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8281 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8341 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8401 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8461 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8521 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8581 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8641 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8701 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8761 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8821 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8881 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8941 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9001 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9061 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9121 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9181 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9241 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9301 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9361 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9421 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9481 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9541 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9601 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9661 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9721 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9781 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9841 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9901 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9961 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
    10021 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    10081 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
    10141 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    10201 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    10261 gtaaaacgac ggccagtgcc aagctg
//
