LOCUS       V038107                10931 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038107
VERSION     V038107
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5359
                     /label="COL6A2(NM_001849)"
                     /note="COL6A2(NM_001849)"
                     /gene="COL6A2"
     CDS             5366..5431
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5474..5685
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5698..5966
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5994..7019
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    7026..7614
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7688..7921
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7993..8114
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8133..8268
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8306..8322)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8330..8346
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8354..8384)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8399..8420
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8708..9296)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9467..10327)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10328..10432)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10906..10922
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc ggatccgcca ccatgctcca gggcacctgc tccgtgctcc tgctctgggg
     2341 aatcctgggg gccatccagg cccagcagca ggaggtcatc tcgccggaca ctaccgagag
     2401 aaacaacaac tgcccagaga agaccgactg ccccatccac gtgtacttcg tgctggacac
     2461 ctcggagagc gtcaccatgc agtcccccac ggacatcctg ctcttccaca tgaagcagtt
     2521 cgtgccgcag ttcatcagcc agctgcagaa cgagttctac ctggaccagg tggcgctgag
     2581 ctggcgctac ggcggcctgc acttctctga ccaggtggag gtgttcagcc caccgggcag
     2641 cgaccgggcc tccttcatca agaacctgca gggcatcagc tccttccgcc gcggcacctt
     2701 caccgactgc gcgctggcca acatgacgga gcagatccgg caggaccgca gcaagggcac
     2761 cgtccacttc gccgtggtca tcaccgacgg ccacgtcacc ggcagcccct gcgggggcat
     2821 caagctgcag gccgagcggg cccgcgagga gggcatccgg ctcttcgccg tggcccccaa
     2881 ccagaacctg aaggagcagg gcctgcggga catcgccagc acgccgcacg agctctaccg
     2941 caacgactac gccaccatgc tgcccgactc caccgagatc gaccaggaca ccatcaaccg
     3001 catcatcaag gtcatgaaac acgaagccta cggagagtgc tacaaggtga gctgcctgga
     3061 aatccctggg ccctctggcc ccaagggcta ccgtggacag aagggtgcca agggcaacat
     3121 gggtgagccg ggagagcctg gccagaaggg aagacaggga gacccgggca tcgaaggccc
     3181 cattggattc ccaggaccca agggcgttcc tggcttcaaa ggagagaagg gtgaatttgg
     3241 agccgacggt cgcaaggggg cccctggcct ggctggcaag aacgggaccg atggacagaa
     3301 gggcaagctg gggcgcatcg gacctcctgg ctgcaaggga gaccctggaa accggggccc
     3361 cgacggttac ccgggggaag cagggagtcc aggggagcga ggagaccaag gcggcaaggg
     3421 ggaccctggc cgcccaggac gcagagggcc cccgggagaa atcggggcca agggaagcaa
     3481 ggggtatcaa ggcaacagtg gagccccagg aagtcctggt gtgaaaggag ccaagggcgg
     3541 gcctgggccc cgcggaccca aaggcgagcc ggggcgcagg ggagaccccg gcaccaaggg
     3601 cagcccaggc agcgatggcc ccaaggggga gaagggggac cctggccctg aggggccccg
     3661 cggcctggct ggagaggttg gcaacaaagg agccaaggga gaccgaggct tgcctggacc
     3721 cagaggcccc cagggagctc ttggggagcc cggaaagcag ggatctcggg gagaccccgg
     3781 tgatgcagga ccccgtggag actcaggaca gccaggcccc aagggagacc ccggcaggcc
     3841 tggattcagc tacccaggac cccgaggagc acccggagaa aaaggcgagc ccggcccacg
     3901 cggccccgag ggaggccgag gcgactttgg cttgaaagga gaacctggga ggaaaggaga
     3961 gaaaggagag cctgcggatc ctggtccccc tggtgagcca ggccctcggg ggccaagagg
     4021 agtcccagga cccgagggtg agcccggccc ccctggagac cccggtctca cggagtgtga
     4081 cgtcatgacc tacgtgaggg agacctgcgg gtgctgcgac tgtgagaagc gctgtggcgc
     4141 cctggacgtg gtcttcgtca tcgacagctc cgagagcatt gggtacacca acttcacact
     4201 ggagaagaac ttcgtcatca acgtggtcaa caggctgggt gccatcgcta aggaccccaa
     4261 gtccgagaca gggacgcgtg tgggcgtggt gcagtacagc cacgagggca cctttgaggc
     4321 catccagctg gacgacgaac gtatcgactc cctgtcgagc ttcaaggagg ctgtcaagaa
     4381 cctcgagtgg attgcgggcg gcacctggac accctcagcc ctcaagtttg cctacgaccg
     4441 cctcatcaag gagagccggc gccagaagac acgtgtgttt gcggtggtca tcacggacgg
     4501 gcgccacgac cctcgggacg atgacctcaa cttgcgggcg ctgtgcgacc gcgacgtcac
     4561 agtgacggcc atcggcatcg gggacatgtt ccacgagaag cacgagagtg aaaacctcta
     4621 ctccatcgcc tgcgacaagc cacagcaggt gcgcaacatg acgctgttct ccgacctggt
     4681 cgctgagaag ttcatcgatg acatggagga cgtcctctgc ccggaccctc agatcgtgtg
     4741 cccagacctt ccctgccaaa cagagctgtc cgtggcacag tgcacgcagc ggcccgtgga
     4801 catcgtcttc ctgctggacg gctccgagcg gctgggtgag cagaacttcc acaaggcccg
     4861 gcgcttcgtg gagcaggtgg cgcggcggct gacgctggcc cggagggacg acgaccctct
     4921 caacgcacgc gtggcgctgc tgcagtttgg tggccccggc gagcagcagg tggccttccc
     4981 gctgagccac aacctcacgg ccatccacga ggcgctggag accacacaat acctgaactc
     5041 cttctcgcac gtgggcgcag gcgtggtgca cgccatcaat gccatcgtgc gcagcccgcg
     5101 tggcggggcc cggaggcacg cagagctgtc cttcgtgttc ctcacggacg gcgtcacggg
     5161 caacgacagt ctgcacgagt cggcgcactc catgcgcaag cagaacgtgg tacccaccgt
     5221 gctggccttg ggcagcgacg tggacatgga cgtgctcacc acgctcagcc tgggtgaccg
     5281 cgccgccgtg ttccacgaga aggactatga cagcctggcg caacccggct tcttcgaccg
     5341 cttcatccgc tggatctgcc tcgaggacta caaagaccat gacggtgatt ataaagatca
     5401 tgacatcgac tacaaggatg acgatgacaa gtagcggccg caaggatctg cgatcgctcc
     5461 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5521 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5581 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5641 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5701 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5761 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5821 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5881 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5941 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgtc gacatgaaaa
     6001 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     6061 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     6121 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     6181 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     6241 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     6301 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     6361 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     6421 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     6481 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     6541 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     6601 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     6661 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     6721 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     6781 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     6841 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     6901 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     6961 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagg
     7021 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7081 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7141 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7201 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7261 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7321 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7381 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7441 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7501 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7561 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     7621 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7681 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     7741 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7801 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7861 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7921 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7981 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8101 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     8161 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8221 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     8281 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8341 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8401 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8521 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8581 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8881 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9121 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9421 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9481 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9541 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9601 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9661 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9721 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9781 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9841 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9901 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9961 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10021 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10081 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10141 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10201 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10261 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10321 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10441 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10501 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    10561 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    10621 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    10681 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    10741 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10801 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10861 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10921 gtgccaagct g
//
