LOCUS       V022371                10372 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022371
VERSION     V022371
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4486
                     /label="Hgf(NM_010427)"
                     /note="Hgf(NM_010427)"
                     /gene="Hgf"
     promoter        4531..4742
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4755..5023
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5052..5061
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5082..5744
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5814..5867
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5868..6467
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6468..7055
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7129..7362
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7434..7555
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7574..7709
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7747..7763)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7771..7787
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7795..7825)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7840..7861
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8149..8737)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8908..9768)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9769..9873)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10347..10363
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgatgtg ggggaccaaa cttctgccgg tcctgttgct
     2341 gcagcatgtc ctcctgcacc tcctcctgct tcatgtcgcc atcccctatg cagaaggaca
     2401 gaagaaaaga agaaatacac ttcatgaatt taaaaagtca gcaaaaacta ctcttaccaa
     2461 ggaagaccca ttactgaaga ttaaaaccaa aaaagtgaac tctgcagatg agtgtgccaa
     2521 caggtgtatc aggaacaggg gctttacgtt cacttgcaag gccttcgttt ttgataagtc
     2581 aagaaaacga tgctactggt atcctttcaa tagtatgtca agtggagtga aaaaagggtt
     2641 tggccatgaa tttgacctct atgaaaacaa agactatatt agaaactgca tcattggtaa
     2701 aggaggcagc tataaaggga cggtatccat cactaagagt ggcatcaaat gccagccttg
     2761 gaattccatg atcccccatg aacacagctt tttgccttcg agctatcgcg gtaaagacct
     2821 acaggaaaac tactgtcgaa atcctcgagg ggaagaaggg ggaccctggt gtttcacaag
     2881 caatccagag gtacgctacg aagtctgtga cattcctcag tgttcagaag ttgaatgcat
     2941 gacctgcaat ggtgaaagct acagaggtcc catggatcac acagaatcag gcaagacttg
     3001 tcagcgctgg gaccagcaga caccacaccg gcacaagttc ttgccagaaa gatatcccga
     3061 caagggcttt gatgataatt attgccgcaa tcctgatggc aagccgaggc catggtgcta
     3121 cactcttgac cctgacaccc cttgggagta ttgtgcaatt aaaacgtgcg ctcacagtgc
     3181 tgtgaatgag actgatgtcc ctatggaaac aactgaatgc attcaaggcc aaggagaagg
     3241 ttacagggga accagcaata ccatttggaa tggaattccc tgtcagcgtt gggattcgca
     3301 gtaccctcac aagcatgata tcactcccga gaacttcaaa tgcaaggacc ttagagaaaa
     3361 ttattgccgc aatccagatg gggctgaatc accatggtgt tttaccactg acccaaacat
     3421 ccgagttggc tactgctctc aaattcccaa gtgtgacgtg tcaagtggac aagattgtta
     3481 tcgtggcaat gggaaaaatt acatgggcaa cttatccaaa acaaggtctg gacttacatg
     3541 ttccatgtgg gacaagaata tggaggattt acaccgtcat atcttctggg agccagatgc
     3601 tagcaaattg aataagaatt actgccggaa tcctgatgat gatgcccatg gaccttggtg
     3661 ctacacgggg aatcctctta ttccttggga ttattgccct atttcccgtt gtgaaggaga
     3721 tactacacct acaattgtca atttggacca tcctgtaata tcctgtgcca aaacaaaaca
     3781 actgcgggtt gtaaatggca ttccaacaca aacaacagta gggtggatgg ttagtttgaa
     3841 atacagaaat aaacatatct gtggaggatc attgataaag gaaagttggg ttcttactgc
     3901 aagacaatgt tttccagcca gaaacaaaga cttgaaagac tatgaagctt ggcttggcat
     3961 ccacgatgtt catgagagag gcgaggagaa gcgcaagcag atcttaaaca tttcccagct
     4021 ggtctatggt cctgaaggct cagacttggt tttactgaag cttgctcgac ctgcaatcct
     4081 ggataacttt gtcagtacaa ttgatttacc tagttatggt tgtacaatcc ctgaaaagac
     4141 cacttgcagt atttacggct ggggctacac tggattgatc aacgcggatg gtttattacg
     4201 agtagctcat ctgtatatta tggggaatga gaaatgcagt cagcaccatc aaggcaaggt
     4261 gactttgaat gagtctgagt tatgtgctgg ggctgaaaag attggatcag gaccatgtga
     4321 gggagattat ggtggcccac tcatttgtga acaacacaaa atgagaatgg ttcttggtgt
     4381 cattgttcct ggtcgtggat gtgccatccc aaatcgtcct ggtatttttg ttcgagtagc
     4441 atattatgca aaatggatac acaaagtaat tttgacatac aagttgtaag cggccgcgaa
     4501 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     4561 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     4621 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     4681 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     4741 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     4801 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     4861 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     4921 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     4981 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     5041 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt
     5101 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca
     5161 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca
     5221 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca
     5281 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca
     5341 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg
     5401 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct
     5461 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata
     5521 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct
     5581 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg
     5641 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca
     5701 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt
     5761 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca
     5821 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca
     5881 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5941 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6001 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6061 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6121 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6181 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6241 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6301 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6361 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6421 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg
     6481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6601 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6661 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6721 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6781 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6841 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6901 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6961 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc
     7021 agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt
     7081 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7141 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7381 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7501 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7561 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7681 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     7741 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7801 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7861 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7921 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8581 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9961 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10021 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10081 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10141 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10201 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10261 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10321 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
