LOCUS       V024212                10310 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024212
VERSION     V024212
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4353
                     /label="Heatr3(NM_172757)"
                     /note="Heatr3(NM_172757)"
                     /gene="Heatr3"
     CDS             4360..4425
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4469..4680
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4693..4961
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4990..4999
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5020..5682
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5752..5805
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5806..6405
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6406..6993
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7067..7300
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7372..7493
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7512..7647
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7685..7701)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7709..7725
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7733..7763)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7778..7799
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8087..8675)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8846..9706)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9707..9811)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10285..10301
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gtaagagtcg gacgaagcgc
     2341 ttcaagcgac ctcagttctc ccctatcgag agctgtcagg ccgaggcggc ggccgcgtcg
     2401 aacgggactg gggacgagga ggacgatggg cccgcggcgg agcttctgga gaagctccag
     2461 cacccgagcg ccgaggtccg cgagtgcgcc tgcgcgggac tggcccggct agtgcagcag
     2521 cggcccgcac taccagactt agcccgtcgg gatgctgtgc gccggctcgg gccgctgctg
     2581 ctggactcga gtctggcggt aagggagacg gcggccggag ctctgagaaa cctcagtgct
     2641 tgtggaggtt ttgacgtttg tgatgacatg gtggcaaagg acatcatgac gcccctggtg
     2701 gcactactga gagagtgtct ctctggactg gattcaaatg agatgtctcc acaggagaaa
     2761 gctgataaga gaaatcctgt agagagcata gccaatgagg ctgtgaatgt gctgtggaat
     2821 gtgtgtgaat gcagtggcag agcagtgtct atattcaaca aagaagggtg tttggagatt
     2881 gtgttacagt acttacgtag gttccccacc agtattgacc tggctgtctc cgtagcgtat
     2941 tgtttacaga cagtgacgga agataaccca gagctcctga aatcttttga tggcacagca
     3001 ctacgtgtgc tggagtctgc tttgctttgt cctgtcgcct ctatggaata cattctttta
     3061 aagacactgg tggcaggcac catctggaac ctaaaggaca ttattccatc taagagtcag
     3121 gcagaaatca ttaatgccat cctaggtgcc ttgtctgaag ttctgggtat gaatactggc
     3181 aacatggtca ttcagatgaa ggaagctgaa acccagcggc taaagactgc cgcagagagt
     3241 gaggacatcc tagcaaatgc taacggtgat gacttggttg aagatgatga aatggaagaa
     3301 atccctcata aaagaaaact cagaaggaaa acatttattt ccgatttact tccacctact
     3361 gacaaggagc tgcgagaggc catggcgttg ctgacagctc agcagactgc tctggaagtt
     3421 attgtcaaca tgtgctgcag tgaagatccc tcggatgatg agtgggaaga actgtctagt
     3481 agtgatgaaa gtgaagcctt tatggaaaac tccttcagtg agtgcagtgg gcacctgatg
     3541 tccccattgt gcctgtccca cgagattcac tctgccctta ccaactgcct cattccagag
     3601 aaggtttttg agaaaacatc ttcaccaaac agtgttgcag ttgacatatg ttctaagaac
     3661 cctacttgga aacctttgat taggaaaatg aacactattc agtgtagagc ccttatgtgt
     3721 ctccagagtc ttgtgtctct cctggacata gaccatctcg gtggagcccc tgctctccag
     3781 actcttgctc agcacctgtc acagattctt ttttctcagc cagattttgc taaacatgat
     3841 gattttctag aagccataag tagtgctttg agggcccttt tgcaaacaat ggcctccaag
     3901 aacattccac agtgtatgac ccctgagcag ctgatgacgc tgtgcagaga aggcattcat
     3961 agcagtagca ttggggttcg agtgaatgtg gtcagtattt tggggatcac tgggagcgtc
     4021 ctagccaaag acgacggcac acttgacact ctgaagacca ttgggtgctt tctgctggaa
     4081 gtcgccacca aagacccttc ccttgtggta acaggcgagg ctctggatgc cctcttcgat
     4141 gtttttgcag atggcgaagc agctgaaaag gcttcagttc aaattaaatt gttagcggct
     4201 ttgaaagaat tccaaccagt cttcaaaatg aagatacgga aagaagggag gggcaagtac
     4261 agtccagacc agctgtgtgt tctcgacaat gtgaagatga atttgagacg gtttattgct
     4321 taccaggaaa ctgttgagaa aagactgact tctggatccg actacaaaga ccatgacggt
     4381 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4441 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4501 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4561 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4621 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4681 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4741 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     4801 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     4861 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     4921 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     4981 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5041 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5101 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5161 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5221 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5281 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5341 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5401 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5461 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5521 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5581 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5641 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5701 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     5761 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     5821 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5881 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5941 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6001 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6061 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6121 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6181 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6241 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6301 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6361 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6421 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6481 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6541 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6601 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6661 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6721 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6781 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6841 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6901 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag
     6961 acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     7021 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7081 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7261 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7321 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7381 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7441 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7501 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7561 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7621 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     7681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8521 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9841 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9901 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9961 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10021 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10081 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10141 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10201 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10261 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
