LOCUS       V033000                10134 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033000
VERSION     V033000
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2297..2306
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2306..2371
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2393..2410
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2450..4411
                     /label="HSPA5(NM_005347)"
                     /note="HSPA5(NM_005347)"
                     /gene="HSPA5"
     CDS             4418..4483
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4527..4738
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4751..5019
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5054..5761
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5771..5824
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5825..6223
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6230..6817
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6891..7124
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7196..7317
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7336..7471
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7509..7525)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7533..7549
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7557..7587)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7602..7623
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7911..8499)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8670..9530)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9531..9635)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10109..10125
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggacta caaagaccat gacggtgatt ataaagatca
     2341 tgatatcgat tacaaggatg acgatgacaa agaatttaat agcgccgtcg accatcatca
     2401 tcatcatcac gaattctgca gatatccatc acactggcgg ccgctcgaga tgaagctctc
     2461 cctggtggcc gcgatgctgc tgctgctcag cgcggcgcgg gccgaggagg aggacaagaa
     2521 ggaggacgtg ggcacggtgg tcggcatcga cctggggacc acctactcct gcgtcggcgt
     2581 gttcaagaac ggccgcgtgg agatcatcgc caacgatcag ggcaaccgca tcacgccgtc
     2641 ctatgtcgcc ttcactcctg aaggggaacg tctgattggc gatgccgcca agaaccagct
     2701 cacctccaac cccgagaaca cggtctttga cgccaagcgg ctcatcggcc gcacgtggaa
     2761 tgacccgtct gtgcagcagg acatcaagtt cttgccgttc aaggtggttg aaaagaaaac
     2821 taaaccatac attcaagttg atattggagg tgggcaaaca aagacatttg ctcctgaaga
     2881 aatttctgcc atggttctca ctaaaatgaa agaaaccgct gaggcttatt tgggaaagaa
     2941 ggttacccat gcagttgtta ctgtaccagc ctattttaat gatgcccaac gccaagcaac
     3001 caaagacgct ggaactattg ctggcctaaa tgttatgagg atcatcaacg agcctacggc
     3061 agctgctatt gcttatggcc tggataagag ggagggggag aagaacatcc tggtgtttga
     3121 cctgggtggc ggaaccttcg atgtgtctct tctcaccatt gacaatggtg tcttcgaagt
     3181 tgtggccact aatggagata ctcatctggg tggagaagac tttgaccagc gtgtcatgga
     3241 acacttcatc aaactgtaca aaaagaagac gggcaaagat gtcaggaaag acaatagagc
     3301 tgtgcagaaa ctccggcgcg aggtagaaaa ggccaaacgg gccctgtctt ctcagcatca
     3361 agcaagaatt gaaattgagt ccttctatga aggagaagac ttttctgaga ccctgactcg
     3421 ggccaaattt gaagagctca acatggatct gttccggtct actatgaagc ccgtccagaa
     3481 agtgttggaa gattctgatt tgaagaagtc tgatattgat gaaattgttc ttgttggtgg
     3541 ctcgactcga attccaaaga ttcagcaact ggttaaagag ttcttcaatg gcaaggaacc
     3601 atcccgtggc ataaacccag atgaagctgt agcgtatggt gctgctgtcc aggctggtgt
     3661 gctctctggt gatcaagata caggtgacct ggtactgctt gatgtatgtc cccttacact
     3721 tggtattgaa actgtgggag gtgtcatgac caaactgatt ccaaggaaca cagtggtgcc
     3781 taccaagaag tctcagatct tttctacagc ttctgataat caaccaactg ttacaatcaa
     3841 ggtctatgaa ggtgaaagac ccctgacaaa agacaatcat cttctgggta catttgatct
     3901 gactggaatt cctcctgctc ctcgtggggt cccacagatt gaagtcacct ttgagataga
     3961 tgtgaatggt attcttcgag tgacagctga agacaagggt acagggaaca aaaataagat
     4021 cacaatcacc aatgaccaga atcgcctgac acctgaagaa atcgaaagga tggttaatga
     4081 tgctgagaag tttgctgagg aagacaaaaa gctcaaggag cgcattgata ctagaaatga
     4141 gttggaaagc tatgcctatt ctctaaagaa tcagattgga gataaagaaa agctgggagg
     4201 taaactttcc tctgaagata aggagaccat ggaaaaagct gtagaagaaa agattgaatg
     4261 gctggaaagc caccaagatg ctgacattga agacttcaaa gctaagaaga aggaactgga
     4321 agaaattgtt caaccaatta tcagcaaact ctatggaagt gcaggccctc ccccaactgg
     4381 tgaagaggat acagcagaaa aagatgagtt gggatccgac tacaaagacc atgacggtga
     4441 ttataaagat catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat
     4501 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4561 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     4621 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     4681 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     4741 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     4801 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     4861 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     4921 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     4981 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     5041 gctagacgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     5101 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     5161 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     5221 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     5281 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     5341 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     5401 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     5461 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     5521 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     5581 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     5641 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     5701 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     5761 gggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     5821 ccctatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     5881 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     5941 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     6001 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     6061 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     6121 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     6181 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgaca atcaacctct
     6241 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6301 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6361 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6421 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6481 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6541 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6601 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6661 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6721 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
     6781 tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac
     6841 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6901 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     6961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7021 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7081 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7141 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7321 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7441 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     7501 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7561 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7621 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7681 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7741 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7801 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8101 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8221 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8281 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8341 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8401 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8461 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8521 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8581 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8641 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8701 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8761 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8821 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8881 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8941 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9001 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9061 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9121 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9181 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9241 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9301 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9361 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9421 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9481 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9661 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9721 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9781 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9841 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9901 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9961 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    10021 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    10081 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
