LOCUS       V021742                 9784 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021742
VERSION     V021742
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..4709
                     /label="HSP90B1(NM_003299)"
                     /note="HSP90B1(NM_003299)"
                     /gene="HSP90B1"
     promoter        4754..4965
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4978..5246
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5273..5872
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5879..6467
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6541..6774
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6846..6967
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6986..7121
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7159..7175)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7183..7199
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7207..7237)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7252..7273
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7561..8149)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8320..9180)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9181..9285)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9759..9775
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atgagggccc tgtgggtgct gggcctctgc tgcgtcctgc
     2341 tgaccttcgg gtcggtcaga gctgacgatg aagttgatgt ggatggtaca gtagaagagg
     2401 atctgggtaa aagtagagaa ggatcaagga cggatgatga agtagtacag agagaggaag
     2461 aagctattca gttggatgga ttaaatgcag cacaaataag agaacttaga gagaagtcgg
     2521 aaaagtttgc cttccaagcc gaagttaaca gaatgatgaa acttatcatc aattcattgt
     2581 ataaaaataa agagattttc ctgagagaac tgatttcaaa tgcttctgat gctttagata
     2641 agataaggct aatatcactg actgatgaaa atgctctttc tggaaatgag gaactaacag
     2701 tcaaaattaa gtgtgataag gagaagaacc tgctgcatgt cacagacacc ggtgtaggaa
     2761 tgaccagaga agagttggtt aaaaaccttg gtaccatagc caaatctggg acaagcgagt
     2821 ttttaaacaa aatgactgaa gcacaggaag atggccagtc aacttctgaa ttgattggcc
     2881 agtttggtgt cggtttctat tccgccttcc ttgtagcaga taaggttatt gtcacttcaa
     2941 aacacaacaa cgatacccag cacatctggg agtctgactc caatgaattt tctgtaattg
     3001 ctgacccaag aggaaacact ctaggacggg gaacgacaat tacccttgtc ttaaaagaag
     3061 aagcatctga ttaccttgaa ttggatacaa ttaaaaatct cgtcaaaaaa tattcacagt
     3121 tcataaactt tcctatttat gtatggagca gcaagactga aactgttgag gagcccatgg
     3181 aggaagaaga agcagccaaa gaagagaaag aagaatctga tgatgaagct gcagtagagg
     3241 aagaagaaga agaaaagaaa ccaaagacta aaaaagttga aaaaactgtc tgggactggg
     3301 aacttatgaa tgatatcaaa ccaatatggc agagaccatc aaaagaagta gaagaagatg
     3361 aatacaaagc tttctacaaa tcattttcaa aggaaagtga tgaccccatg gcttatattc
     3421 actttactgc tgaaggggaa gttaccttca aatcaatttt atttgtaccc acatctgctc
     3481 cacgtggtct gtttgacgaa tatggatcta aaaagagcga ttacattaag ctctatgtgc
     3541 gccgtgtatt catcacagac gacttccatg atatgatgcc taaatacctc aattttgtca
     3601 agggtgtggt ggactcagat gatctcccct tgaatgtttc ccgcgagact cttcagcaac
     3661 ataaactgct taaggtgatt aggaagaagc ttgttcgtaa aacgctggac atgatcaaga
     3721 agattgctga tgataaatac aatgatactt tttggaaaga atttggtacc aacatcaagc
     3781 ttggtgtgat tgaagaccac tcgaatcgaa cacgtcttgc taaacttctt aggttccagt
     3841 cttctcatca tccaactgac attactagcc tagaccagta tgtggaaaga atgaaggaaa
     3901 aacaagacaa aatctacttc atggctgggt ccagcagaaa agaggctgaa tcttctccat
     3961 ttgttgagcg acttctgaaa aagggctatg aagttattta cctcacagaa cctgtggatg
     4021 aatactgtat tcaggccctt cccgaatttg atgggaagag gttccagaat gttgccaagg
     4081 aaggagtgaa gttcgatgaa agtgagaaaa ctaaggagag tcgtgaagca gttgagaaag
     4141 aatttgagcc tctgctgaat tggatgaaag ataaagccct taaggacaag attgaaaagg
     4201 ctgtggtgtc tcagcgcctg acagaatctc cgtgtgcttt ggtggccagc cagtacggat
     4261 ggtctggcaa catggagaga atcatgaaag cacaagcgta ccaaacgggc aaggacatct
     4321 ctacaaatta ctatgcgagt cagaagaaaa catttgaaat taatcccaga cacccgctga
     4381 tcagagacat gcttcgacga attaaggaag atgaagatga taaaacagtt ttggatcttg
     4441 ctgtggtttt gtttgaaaca gcaacgcttc ggtcagggta tcttttacca gacactaaag
     4501 catatggaga tagaatagaa agaatgcttc gcctcagttt gaacattgac cctgatgcaa
     4561 aggtggaaga agagcccgaa gaagaacctg aagagacagc agaagacaca acagaagaca
     4621 cagagcaaga cgaagatgaa gaaatggatg tgggaacaga tgaagaagaa gaaacagcaa
     4681 aggaatctac agctgaaaaa gatgaattgt aagcggccgc gaaggatctg cgatcgctcc
     4741 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4801 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4861 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4921 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     4981 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5041 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5101 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5161 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5221 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     5281 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     5341 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     5401 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5461 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5521 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5581 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5641 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5701 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5761 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5821 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     5881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6241 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6301 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     6481 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6541 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     6601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6781 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6841 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6961 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     7021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     7141 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7201 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7261 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7321 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8281 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8341 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8401 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8461 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8521 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8581 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8641 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8701 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8761 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8821 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8881 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8941 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9001 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9061 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9121 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9181 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9241 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9301 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9361 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     9541 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     9601 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     9661 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     9721 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     9781 gctg
//
