LOCUS V021742 9784 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021742 VERSION V021742 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2301..4709 /label="HSP90B1(NM_003299)" /note="HSP90B1(NM_003299)" /gene="HSP90B1" promoter 4754..4965 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4978..5246 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5273..5872 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5879..6467 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6541..6774 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6846..6967 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6986..7121 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7159..7175) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7183..7199 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7207..7237) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7252..7273 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7561..8149) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8320..9180) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9181..9285) /gene="bla" /label="AmpR promoter" primer_bind 9759..9775 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta 2281 gagctagcga attcgccacc atgagggccc tgtgggtgct gggcctctgc tgcgtcctgc 2341 tgaccttcgg gtcggtcaga gctgacgatg aagttgatgt ggatggtaca gtagaagagg 2401 atctgggtaa aagtagagaa ggatcaagga cggatgatga agtagtacag agagaggaag 2461 aagctattca gttggatgga ttaaatgcag cacaaataag agaacttaga gagaagtcgg 2521 aaaagtttgc cttccaagcc gaagttaaca gaatgatgaa acttatcatc aattcattgt 2581 ataaaaataa agagattttc ctgagagaac tgatttcaaa tgcttctgat gctttagata 2641 agataaggct aatatcactg actgatgaaa atgctctttc tggaaatgag gaactaacag 2701 tcaaaattaa gtgtgataag gagaagaacc tgctgcatgt cacagacacc ggtgtaggaa 2761 tgaccagaga agagttggtt aaaaaccttg gtaccatagc caaatctggg acaagcgagt 2821 ttttaaacaa aatgactgaa gcacaggaag atggccagtc aacttctgaa ttgattggcc 2881 agtttggtgt cggtttctat tccgccttcc ttgtagcaga taaggttatt gtcacttcaa 2941 aacacaacaa cgatacccag cacatctggg agtctgactc caatgaattt tctgtaattg 3001 ctgacccaag aggaaacact ctaggacggg gaacgacaat tacccttgtc ttaaaagaag 3061 aagcatctga ttaccttgaa ttggatacaa ttaaaaatct cgtcaaaaaa tattcacagt 3121 tcataaactt tcctatttat gtatggagca gcaagactga aactgttgag gagcccatgg 3181 aggaagaaga agcagccaaa gaagagaaag aagaatctga tgatgaagct gcagtagagg 3241 aagaagaaga agaaaagaaa ccaaagacta aaaaagttga aaaaactgtc tgggactggg 3301 aacttatgaa tgatatcaaa ccaatatggc agagaccatc aaaagaagta gaagaagatg 3361 aatacaaagc tttctacaaa tcattttcaa aggaaagtga tgaccccatg gcttatattc 3421 actttactgc tgaaggggaa gttaccttca aatcaatttt atttgtaccc acatctgctc 3481 cacgtggtct gtttgacgaa tatggatcta aaaagagcga ttacattaag ctctatgtgc 3541 gccgtgtatt catcacagac gacttccatg atatgatgcc taaatacctc aattttgtca 3601 agggtgtggt ggactcagat gatctcccct tgaatgtttc ccgcgagact cttcagcaac 3661 ataaactgct taaggtgatt aggaagaagc ttgttcgtaa aacgctggac atgatcaaga 3721 agattgctga tgataaatac aatgatactt tttggaaaga atttggtacc aacatcaagc 3781 ttggtgtgat tgaagaccac tcgaatcgaa cacgtcttgc taaacttctt aggttccagt 3841 cttctcatca tccaactgac attactagcc tagaccagta tgtggaaaga atgaaggaaa 3901 aacaagacaa aatctacttc atggctgggt ccagcagaaa agaggctgaa tcttctccat 3961 ttgttgagcg acttctgaaa aagggctatg aagttattta cctcacagaa cctgtggatg 4021 aatactgtat tcaggccctt cccgaatttg atgggaagag gttccagaat gttgccaagg 4081 aaggagtgaa gttcgatgaa agtgagaaaa ctaaggagag tcgtgaagca gttgagaaag 4141 aatttgagcc tctgctgaat tggatgaaag ataaagccct taaggacaag attgaaaagg 4201 ctgtggtgtc tcagcgcctg acagaatctc cgtgtgcttt ggtggccagc cagtacggat 4261 ggtctggcaa catggagaga atcatgaaag cacaagcgta ccaaacgggc aaggacatct 4321 ctacaaatta ctatgcgagt cagaagaaaa catttgaaat taatcccaga cacccgctga 4381 tcagagacat gcttcgacga attaaggaag atgaagatga taaaacagtt ttggatcttg 4441 ctgtggtttt gtttgaaaca gcaacgcttc ggtcagggta tcttttacca gacactaaag 4501 catatggaga tagaatagaa agaatgcttc gcctcagttt gaacattgac cctgatgcaa 4561 aggtggaaga agagcccgaa gaagaacctg aagagacagc agaagacaca acagaagaca 4621 cagagcaaga cgaagatgaa gaaatggatg tgggaacaga tgaagaagaa gaaacagcaa 4681 aggaatctac agctgaaaaa gatgaattgt aagcggccgc gaaggatctg cgatcgctcc 4741 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4801 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4861 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4921 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4981 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 5041 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 5101 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 5161 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 5221 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga 5281 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 5341 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 5401 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5461 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5521 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5581 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5641 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5701 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5761 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5821 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa 5881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 6001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 6061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 6121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 6181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 6241 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 6301 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 6361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 6421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 6481 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6541 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6781 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6841 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6961 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 7021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 7081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 7141 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7201 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7261 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7321 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 8041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8281 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8341 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8401 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8461 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8521 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8581 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8641 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8701 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8761 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8821 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8881 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8941 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9001 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9061 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9121 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9181 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9241 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9301 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9361 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9541 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9601 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9661 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9721 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9781 gctg //