LOCUS       V033534                10954 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033534
VERSION     V033534
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4980
                     /label="HMGCR(NM_000859)"
                     /note="HMGCR(NM_000859)"
                     /gene="HMGCR"
     CDS             4987..5052
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5096..5307
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5320..5588
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5617..5626
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5647..6309
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6379..6432
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6433..7032
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7033..7621
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7695..7928
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8000..8121
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8161..8296
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8329..8345)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8353..8369
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8377..8407)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8422..8443
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8731..9319)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9490..10350)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10351..10455)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10929..10945
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tgtcaagact ttttcgaatg
     2341 catggcctct ttgtggcctc ccatccctgg gaagtcatag tggggacagt gacactgacc
     2401 atctgcatga tgtccatgaa catgtttact ggtaacaata agatctgtgg ttggaattat
     2461 gaatgtccaa agtttgaaga ggatgttttg agcagtgaca ttataattct gacaataaca
     2521 cgatgcatag ccatcctgta tatttacttc cagttccaga atttacgtca acttggatca
     2581 aaatatattt tgggtattgc tggccttttc acaattttct caagttttgt attcagtaca
     2641 gttgtcattc acttcttaga caaagaattg acaggcttga atgaagcttt gccctttttc
     2701 ctacttttga ttgacctttc cagagcaagc acattagcaa agtttgccct cagttccaac
     2761 tcacaggatg aagtaaggga aaatattgct cgtggaatgg caattttagg tcctacgttt
     2821 accctcgatg ctcttgttga atgtcttgtg attggagttg gtaccatgtc aggggtacgt
     2881 cagcttgaaa ttatgtgctg ctttggctgc atgtcagttc ttgccaacta cttcgtgttc
     2941 atgactttct tcccagcttg tgtgtccttg gtattagagc tttctcggga aagccgcgag
     3001 ggtcgtccaa tttggcagct cagccatttt gcccgagttt tagaagaaga agaaaataag
     3061 ccgaatcctg taactcagag ggtcaagatg attatgtctc taggcttggt tcttgttcat
     3121 gctcacagtc gctggatagc tgatccttct cctcaaaaca gtacagcaga tacttctaag
     3181 gtttcattag gactggatga aaatgtgtcc aagagaattg aaccaagtgt ttccctctgg
     3241 cagttttatc tctctaaaat gatcagcatg gatattgaac aagttattac cctaagttta
     3301 gctctccttc tggctgtcaa gtacatcttc tttgaacaaa cagagacaga atctacactc
     3361 tcattaaaaa accctatcac atctcctgta gtgacacaaa agaaagtccc agacaattgt
     3421 tgtagacgtg aacctatgct ggtcagaaat aaccagaaat gtgattcagt agaggaagag
     3481 acagggataa accgagaaag aaaagttgag gttataaaac ccttagtggc tgaaacagat
     3541 accccaaaca gagctacatt tgtggttggt aactcctcct tactcgatac ttcatcagta
     3601 ctggtgacac aggaacctga aattgaactt cccagggaac ctcggcctaa tgaagaatgt
     3661 ctacagatac ttgggaatgc agagaaaggt gcaaaattcc ttagtgatgc tgagatcatc
     3721 cagttagtca atgctaagca tatcccagcc tacaagttgg aaactctgat ggaaactcat
     3781 gagcgtggtg tatctattcg ccgacagtta ctttccaaga agctttcaga accttcttct
     3841 ctccagtacc taccttacag ggattataat tactccttgg tgatgggagc ttgttgtgag
     3901 aatgttattg gatatatgcc catccctgtt ggagtggcag gacccctttg cttagatgaa
     3961 aaagaatttc aggttccaat ggcaacaaca gaaggttgtc ttgtggccag caccaataga
     4021 ggctgcagag caataggtct tggtggaggt gccagcagcc gagtccttgc agatgggatg
     4081 actcgtggcc cagttgtgcg tcttccacgt gcttgtgact ctgcagaagt gaaagcctgg
     4141 ctcgaaacat ctgaagggtt cgcagtgata aaggaggcat ttgacagcac tagcagattt
     4201 gcacgtctac agaaacttca tacaagtata gctggacgca acctttatat ccgtttccag
     4261 tccaggtcag gggatgccat ggggatgaac atgatttcaa agggtacaga gaaagcactt
     4321 tcaaaacttc acgagtattt ccctgaaatg cagattctag ccgttagtgg taactattgt
     4381 actgacaaga aacctgctgc tataaattgg atagagggaa gaggaaaatc tgttgtttgt
     4441 gaagctgtca ttccagccaa ggttgtcaga gaagtattaa agactaccac agaggctatg
     4501 attgaggtca acattaacaa gaatttagtg ggctctgcca tggctgggag cataggaggc
     4561 tacaacgccc atgcagcaaa cattgtcacc gccatctaca ttgcctgtgg acaggatgca
     4621 gcacagaatg ttggtagttc aaactgtatt actttaatgg aagcaagtgg tcccacaaat
     4681 gaagatttat atatcagctg caccatgcca tctatagaga taggaacggt gggtggtggg
     4741 accaacctac tacctcagca agcctgtttg cagatgctag gtgttcaagg agcatgcaaa
     4801 gataatcctg gggaaaatgc ccggcagctt gcccgaattg tgtgtgggac cgtaatggct
     4861 ggggaattgt cacttatggc agcattggca gcaggacatc ttgtcaaaag tcacatgatt
     4921 cacaacaggt cgaagatcaa tttacaagac ctccaaggag cttgcaccaa gaagacagcc
     4981 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     5041 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     5101 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     5161 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     5221 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     5281 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     5341 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     5401 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5461 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5521 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5581 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5641 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5701 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5761 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5821 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5881 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5941 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     6001 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     6061 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     6121 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     6181 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     6241 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     6301 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     6361 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6421 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6481 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6541 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6601 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6661 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6721 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6781 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6841 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6901 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6961 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7021 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7081 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7141 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7201 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7261 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7321 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7381 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7441 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7501 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7561 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7621 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7681 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7741 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7801 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7861 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7921 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7981 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8101 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8161 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8221 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8281 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     8341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8401 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8461 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8521 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8641 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8701 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8761 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8821 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8881 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8941 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9001 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9061 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9301 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9361 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9421 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9481 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9601 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10381 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10441 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10501 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10561 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10621 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10681 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10741 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10801 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10861 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10921 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
