LOCUS       V041466                11362 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041466
VERSION     V041466
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5388
                     /label="HERC5(NM_016323)"
                     /note="HERC5(NM_016323)"
                     /gene="HERC5"
     CDS             5395..5460
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5504..5715
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5728..5996
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6025..6034
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6055..6717
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6787..6840
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6841..7440
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7441..8029
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8103..8336
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8408..8529
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8569..8704
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8737..8753)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8761..8777
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8785..8815)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8830..8851
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9139..9727)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9898..10758)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10759..10863)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11337..11353
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agcggaggtc gcggaggaag
     2341 tcgcggcgca acgggcgctc gaccgcgggc aaggccgccg cgacccagcc cgcgaagtct
     2401 ccgggcgcac agctctggct ctttcccagc gccgcgggcc tccaccgcgc gctgctccgg
     2461 agggtggagg tgacgcgcca actctgctgc tcgccggggc gcctcgcggt cttggaacgc
     2521 ggcggggcgg gcgtccaggt tcaccagctg ctcgccggga gcggcggcgc ccggacgccg
     2581 aaatgcatta aattaggaaa aaacatgaag atacattccg tggaccaagg agcagagcac
     2641 atgctgattc tctcatcaga tggaaaacca tttgagtatg acaactatag catgaaacat
     2701 ctaaggtttg aaagcatttt acaagaaaaa aaaataattc agatcacatg tggagattac
     2761 cattctcttg cactctcaaa aggtggtgag ctttttgcct ggggacagaa cctgcatggg
     2821 cagcttggag ttggaaggaa atttccctca accaccacac cacagattgt ggagcacctc
     2881 gcaggagtac ccttggctca gatttctgcc ggagaagccc acagcatggc cttatccatg
     2941 tctggcaaca tttattcatg gggaaaaaat gaatgtggac aactaggcct gggccacact
     3001 gagagtaaag atgatccatc ccttattgaa ggactagaca atcagaaagt tgaatttgtc
     3061 gcttgtggtg gctctcacag tgccctactc acacaggatg ggctgctgtt tactttcggt
     3121 gctggaaaac atgggcaact tggtcataat tcaacacaga atgagctaag accctgtttg
     3181 gtggctgagc ttgttgggta tagagtgact cagatagcat gtggaaggtg gcacacactt
     3241 gcctatgttt ctgatttggg aaaggtcttt tcctttggtt ctggaaaaga tggacaactg
     3301 ggaaatggtg gaacacgtga ccagctgatg ccgcttccag tgaaagtatc atcaagtgaa
     3361 gaactcaaac ttgaaagcca tacctcagaa aaggagttaa taatgattgc tggagggaat
     3421 caaagcattt tgctctggat aaagaaagag aattcatatg ttaatctgaa gaggacaatt
     3481 cctactctga atgaagggac tgtaaagaga tggattgctg atgtggagac taaacggtgg
     3541 cagagcacaa aaagggaaat ccaagagata ttttcatctc ctgcttgtct aactggaagt
     3601 tttttaagga aaagaagaac tacagaaatg atgcctgttt atttggactt aaataaagca
     3661 agaaacatct tcaaggagtt aacccaaaag gactggatta ctaacatgat aaccacctgc
     3721 ctcaaagata atctgctcaa aagacttcca tttcattctc caccccaaga agctttagaa
     3781 attttcttcc ttctcccaga atgtcctatg atgcatattt ccaacaactg ggagagcctt
     3841 gtggttccat ttgcaaaggt tgtttgtaaa atgagtgacc agtcttcact ggttctggaa
     3901 gagtattggg caactctgca agaatccact ttcagcaaac tggtccagat gtttaaaaca
     3961 gccgtcatat gccagttgga ttactgggat gaaagtgctg aggagaatgg taatgttcaa
     4021 gctctcctag aaatgttgaa gaagctgcac agggtaaacc aggtgaaatg tcaactacct
     4081 gaaagtattt tccaagtaga cgaactcttg caccgtctca atttttttgt agaagtatgc
     4141 agaaggtact tgtggaaaat gactgtggac gcttcagaaa atgtacaatg ctgcgtcata
     4201 ttcagtcact ttccatttat ctttaataat ctgtcgaaaa ttaaactact acatacagac
     4261 acacttttaa aaatagagag taaaaaacat aaagcttatc ttaggtcggc agcaattgag
     4321 gaagaaagag agtctgaatt cgctttgagg cccacgtttg atctaacagt cagaaggaat
     4381 cacttgattg aggatgtttt gaatcagcta agtcaatttg agaatgaaga cctgaggaaa
     4441 gagttatggg tttcatttag tggagaaatt gggtatgacc tcggaggagt caagaaagag
     4501 ttcttctact gtctgtttgc agagatgatc cagccggaat atgggatgtt catgtatcct
     4561 gaaggggctt cctgcatgtg gtttcctgtc aagcctaaat ttgagaagaa aagatacttc
     4621 ttttttgggg ttctatgtgg actttccctg ttcaattgca atgttgccaa ccttcctttc
     4681 ccactggcac tgtttaagaa acttttggac caaatgccat cattggaaga cttgaaagaa
     4741 ctcagtcctg atttgggaaa gaatttgcaa acacttctgg atgatgaagg tgataacttt
     4801 gaggaagtat tttacatcca ttttaatgtg cactgggaca gaaacgacac aaacttaatt
     4861 cctaatggaa gtagcataac tgtcaaccag actaacaaga gagactatgt ttctaagtat
     4921 atcaattaca ttttcaacga ctctgtaaag gcggtttatg aagaatttcg gagaggattt
     4981 tataaaatgt gcgacgaaga cattatcaaa ttattccacc ccgaagaact gaaggatgtg
     5041 attgttggaa atacagatta tgattggaaa acatttgaaa agaatgcacg ttatgaacca
     5101 ggatataaca gttcacatcc caccatagtg atgttttgga aggctttcca caaattgact
     5161 ctggaagaaa agaaaaaatt ccttgtattt cttacaggaa ctgacagact acaaatgaaa
     5221 gatttaaata atatgaaaat aacattttgc tgtcctgaaa gttggaatga aagagaccct
     5281 ataagagcac tgacatgttt cagtgtcctc ttcctcccta aatattctac aatggaaaca
     5341 gttgaagaag cgcttcaaga agccatcaac aacaacagag gatttggcgg atccgactac
     5401 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     5461 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5521 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5581 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5641 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5701 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5761 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5821 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5881 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5941 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     6001 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     6061 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     6121 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     6181 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     6241 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     6301 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     6361 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     6421 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     6481 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     6541 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     6601 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     6661 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6721 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6781 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6841 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6901 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6961 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     7021 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     7081 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     7141 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     7201 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     7261 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7321 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7381 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     7441 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7501 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7561 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7621 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7681 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7741 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7801 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7861 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7921 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7981 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     8041 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8101 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     8161 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8221 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8281 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8341 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8401 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8461 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8521 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8641 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8701 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     8761 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8821 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8881 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9541 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9841 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9901 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9961 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10021 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10081 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10141 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10201 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10261 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10321 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10381 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10441 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10501 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10561 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10621 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10681 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10741 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10801 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10861 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10921 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10981 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11041 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    11101 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    11161 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    11221 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    11281 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11341 aacgacggcc agtgccaagc tg
//
