LOCUS       V037599                11271 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037599
VERSION     V037599
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5290
                     /label="GTF2I(NM_032999)"
                     /note="GTF2I(NM_032999)"
                     /gene="GTF2I"
     CDS             5297..5386
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5430..5641
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5654..5922
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5951..5960
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5981..6643
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6713..6766
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6767..7366
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7367..7954
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8028..8261
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8333..8454
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8473..8608
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8646..8662)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8670..8686
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8694..8724)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8739..8760
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9048..9636)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9807..10667)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10668..10772)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11246..11262
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg cccaagttgc aatgtccacc ctccccgttg aagatgagga
     2341 gtcctcggag agcaggatgg tggtgacatt cctcatgtca gctctcgagt ccatgtgtaa
     2401 agaactggcc aagtccaaag ccgaagtggc ctgcattgca gtgtatgaaa cagacgtgtt
     2461 tgtcgtcgga actgaaagag gacgtgcttt tgtcaatacc agaaaggatt ttcaaaaaga
     2521 ttttgtaaaa tattgtgttg aagaagaaga aaaagctgca gagatgcata aaatgaaatc
     2581 tacaacccag gcaaatcgga tgagtgtaga tgctgtagaa attgaaacac tcagaaaaac
     2641 agttgaggac tatttctgct tttgctatgg gaaagcttta ggcaaatcca cagtggtacc
     2701 tgtaccatat gagaagatgc tgcgagacca gtcggctgtg gtagtgcagg ggcttccgga
     2761 aggtgttgcc tttaaacacc ccgagaacta tgatcttgca accctgaaat ggattttgga
     2821 gaacaaagca gggatttcat tcatcattaa gagacctttt ttagagccaa agaagcatgt
     2881 aggtggtcgt gtgatggtaa cagatgctga caggtcaata ctatctccag gtggaagttg
     2941 tggccccatc aaagtgaaaa ctgaacccac agaagattct ggcatttccc tggaaatggc
     3001 agctgtgaca gtaaaggaag aatcagaaga tcctgattat tatcaatata acattcaagc
     3061 aggcccttct gaaactgatg atgttgatga aaaacagccc ctatcgaagc ctttgcaagg
     3121 aagccaccat tcttcagagg gcaatgaagg cacagaaatg gaagtaccag cagaagattc
     3181 tactcaacat gtcccttcag aaacaagtga ggaccctgaa gttgaggtga ctattgaaga
     3241 tgatgattat tctccaccgt ctaagagacc aaaggccaat gagctaccgc agccaccagt
     3301 cccggaaccc gccaatgctg ggaagcggaa agtgagggag ttcaacttcg agaaatggaa
     3361 tgctcgcatc actgatctac gtaaacaagt tgaagaattg tttgaaagga aatatgctca
     3421 agccataaaa gccaaaggtc cggtgacgat cccgtaccct cttttccagt ctcatgttga
     3481 agatctttat gtagaaggac ttcctgaagg aattcctttt agaaggccat ctacttacgg
     3541 aattcctcgc ctggagagga tattacttgc aaaggaaagg attcgttttg tgattaagaa
     3601 acatgagctt ctgaattcaa cacgtgaaga tttacagctt gataagccag cttcaggagt
     3661 aaaggaagaa tggtatgcca gaatcactaa attaagaaag atggtggatc agcttttctg
     3721 caaaaaattt gcggaagcct tggggagcac tgaagccaag gctgtaccgt accaaaaatt
     3781 tgaggcacac ccgaatgatc tgtacgtgga aggactgcca gaaaacattc ctttccgaag
     3841 tccctcatgg tatggaatcc caaggctgga aaaaatcatt caagtgggca atcgaattaa
     3901 atttgttatt aaaagaccag aacttctgac tcacagtacc actgaagtta ctcagccaag
     3961 aacgaataca ccagtcaaag aagattggaa tgtcagaatt accaagctac ggaagcaagt
     4021 ggaagagatt tttaatttga aatttgctca agctcttgga ctcaccgagg cagtaaaagt
     4081 accatatcct gtgtttgaat caaacccgga gttcttgtat gtggaaggct tgccagaggg
     4141 gattcccttc cgaagcccta cctggtttgg aattccacga cttgaaagga tcgtccgcgg
     4201 gagtaataaa atcaagttcg ttgttaaaaa acctgaacta gttatttcct acttgcctcc
     4261 tgggatggct agtaaaataa acactaaagc tttgcagtcc cccaaaagac cacgaagtcc
     4321 tgggagtaat tcaaaggttc ctgaaattga ggtcaccgtg gaaggcccta ataacaacaa
     4381 tcctcaaacc tcagctgttc gaaccccgac ccagactaac ggttctaacg ttcccttcaa
     4441 gccacgaggg agagagtttt cctttgaggc ctggaatgcc aaaatcacgg acctaaaaca
     4501 gaaagttgaa aatctcttca atgagaaatg tggggaagct cttggcctta aacaagctgt
     4561 gaaggtgccg ttcgcgttat ttgagtcttt cccggaagac ttttatgtgg aaggcttacc
     4621 tgagggtgtg ccattccgaa gaccatcgac ttttggcatt ccgaggctgg agaagatact
     4681 cagaaacaaa gccaaaatta agttcatcat taaaaagccc gaaatgtttg agacggcgat
     4741 taaggagagc acctcctcta agagccctcc cagaaaaata aattcatcac ccaatgttaa
     4801 tactactgca tcaggtgttg aagaccttaa catcattcag gtgacaattc cagatgatga
     4861 taatgaaaga ctctcgaaag ttgaaaaagc tagacagcta agagaacaag tgaatgacct
     4921 ctttagtcgg aaatttggtg aagctattgg tatgggtttt cctgtgaaag ttccctacag
     4981 gaaaatcaca attaaccctg gctgtgtggt agttgatggc atgcccccgg gggtgtcctt
     5041 caaagccccc agctacctgg aaatcagctc catgagaagg atcttagact ctgccgagtt
     5101 tatcaaattc acggtcatta gaccatttcc aggacttgtg attaataacc agctggttga
     5161 tcagagtgag tcagaaggcc ccgtgataca agaatcagct gaaccaagcc agttggaagt
     5221 tccagccaca gaagaaataa aagagactga tggaagctct cagatcaagc aagaaccaga
     5281 ccccacgtgg ggatccgaac aaaaactaat atcagaggaa gatttggaac agaaactaat
     5341 cagtgaagaa gatttagagc aaaagttaat ttccgaggag gacttatagc ggccgcgaag
     5401 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5461 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5521 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5581 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5641 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5701 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5761 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5821 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5881 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     5941 tacgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg
     6001 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     6061 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     6121 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     6181 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     6241 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     6301 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     6361 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     6421 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     6481 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     6541 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     6601 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc
     6661 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag
     6721 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     6781 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6841 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6901 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6961 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7021 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7081 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7141 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7201 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7261 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7321 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga
     7381 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7441 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7501 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7561 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7621 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7681 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7741 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7801 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7861 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca
     7921 gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta
     7981 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8041 tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8101 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8161 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8221 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8281 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8461 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8521 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8581 aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa
     8641 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8701 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8761 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8821 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8881 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8941 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9481 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10801 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10861 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    10921 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10981 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    11041 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    11101 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    11161 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    11221 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
