LOCUS       V033486                11284 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033486
VERSION     V033486
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5310
                     /label="GTF2I(NM_032999)"
                     /note="GTF2I(NM_032999)"
                     /gene="GTF2I"
     CDS             5317..5382
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5426..5637
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5650..5918
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5947..5956
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5977..6639
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6709..6762
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6763..7362
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7363..7951
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8025..8258
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8330..8451
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8491..8626
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8659..8675)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8683..8699
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8707..8737)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8752..8773
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9061..9649)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9820..10680)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10681..10785)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11259..11275
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cccaagttgc aatgtccacc
     2341 ctccccgttg aagatgagga gtcctcggag agcaggatgg tggtgacatt cctcatgtca
     2401 gctctcgagt ccatgtgtaa agaactggcc aagtccaaag ccgaagtggc ctgcattgca
     2461 gtgtatgaaa cagacgtgtt tgtcgtcgga actgaaagag gacgtgcttt tgtcaatacc
     2521 agaaaggatt ttcaaaaaga ttttgtaaaa tattgtgttg aagaagaaga aaaagctgca
     2581 gagatgcata aaatgaaatc tacaacccag gcaaatcgga tgagtgtaga tgctgtagaa
     2641 attgaaacac tcagaaaaac agttgaggac tatttctgct tttgctatgg gaaagcttta
     2701 ggcaaatcca cagtggtacc tgtaccatat gagaagatgc tgcgagacca gtcggctgtg
     2761 gtagtgcagg ggcttccgga aggtgttgcc tttaaacacc ccgagaacta tgatcttgca
     2821 accctgaaat ggattttgga gaacaaagca gggatttcat tcatcattaa gagacctttt
     2881 ttagagccaa agaagcatgt aggtggtcgt gtgatggtaa cagatgctga caggtcaata
     2941 ctatctccag gtggaagttg tggccccatc aaagtgaaaa ctgaacccac agaagattct
     3001 ggcatttccc tggaaatggc agctgtgaca gtaaaggaag aatcagaaga tcctgattat
     3061 tatcaatata acattcaagc aggcccttct gaaactgatg atgttgatga aaaacagccc
     3121 ctatcgaagc ctttgcaagg aagccaccat tcttcagagg gcaatgaagg cacagaaatg
     3181 gaagtaccag cagaagattc tactcaacat gtcccttcag aaacaagtga ggaccctgaa
     3241 gttgaggtga ctattgaaga tgatgattat tctccaccgt ctaagagacc aaaggccaat
     3301 gagctaccgc agccaccagt cccggaaccc gccaatgctg ggaagcggaa agtgagggag
     3361 ttcaacttcg agaaatggaa tgctcgcatc actgatctac gtaaacaagt tgaagaattg
     3421 tttgaaagga aatatgctca agccataaaa gccaaaggtc cggtgacgat cccgtaccct
     3481 cttttccagt ctcatgttga agatctttat gtagaaggac ttcctgaagg aattcctttt
     3541 agaaggccat ctacttacgg aattcctcgc ctggagagga tattacttgc aaaggaaagg
     3601 attcgttttg tgattaagaa acatgagctt ctgaattcaa cacgtgaaga tttacagctt
     3661 gataagccag cttcaggagt aaaggaagaa tggtatgcca gaatcactaa attaagaaag
     3721 atggtggatc agcttttctg caaaaaattt gcggaagcct tggggagcac tgaagccaag
     3781 gctgtaccgt accaaaaatt tgaggcacac ccgaatgatc tgtacgtgga aggactgcca
     3841 gaaaacattc ctttccgaag tccctcatgg tatggaatcc caaggctgga aaaaatcatt
     3901 caagtgggca atcgaattaa atttgttatt aaaagaccag aacttctgac tcacagtacc
     3961 actgaagtta ctcagccaag aacgaataca ccagtcaaag aagattggaa tgtcagaatt
     4021 accaagctac ggaagcaagt ggaagagatt tttaatttga aatttgctca agctcttgga
     4081 ctcaccgagg cagtaaaagt accatatcct gtgtttgaat caaacccgga gttcttgtat
     4141 gtggaaggct tgccagaggg gattcccttc cgaagcccta cctggtttgg aattccacga
     4201 cttgaaagga tcgtccgcgg gagtaataaa atcaagttcg ttgttaaaaa acctgaacta
     4261 gttatttcct acttgcctcc tgggatggct agtaaaataa acactaaagc tttgcagtcc
     4321 cccaaaagac cacgaagtcc tgggagtaat tcaaaggttc ctgaaattga ggtcaccgtg
     4381 gaaggcccta ataacaacaa tcctcaaacc tcagctgttc gaaccccgac ccagactaac
     4441 ggttctaacg ttcccttcaa gccacgaggg agagagtttt cctttgaggc ctggaatgcc
     4501 aaaatcacgg acctaaaaca gaaagttgaa aatctcttca atgagaaatg tggggaagct
     4561 cttggcctta aacaagctgt gaaggtgccg ttcgcgttat ttgagtcttt cccggaagac
     4621 ttttatgtgg aaggcttacc tgagggtgtg ccattccgaa gaccatcgac ttttggcatt
     4681 ccgaggctgg agaagatact cagaaacaaa gccaaaatta agttcatcat taaaaagccc
     4741 gaaatgtttg agacggcgat taaggagagc acctcctcta agagccctcc cagaaaaata
     4801 aattcatcac ccaatgttaa tactactgca tcaggtgttg aagaccttaa catcattcag
     4861 gtgacaattc cagatgatga taatgaaaga ctctcgaaag ttgaaaaagc tagacagcta
     4921 agagaacaag tgaatgacct ctttagtcgg aaatttggtg aagctattgg tatgggtttt
     4981 cctgtgaaag ttccctacag gaaaatcaca attaaccctg gctgtgtggt agttgatggc
     5041 atgcccccgg gggtgtcctt caaagccccc agctacctgg aaatcagctc catgagaagg
     5101 atcttagact ctgccgagtt tatcaaattc acggtcatta gaccatttcc aggacttgtg
     5161 attaataacc agctggttga tcagagtgag tcagaaggcc ccgtgataca agaatcagct
     5221 gaaccaagcc agttggaagt tccagccaca gaagaaataa aagagactga tggaagctct
     5281 cagatcaagc aagaaccaga ccccacgtgg ggatccgact acaaagacca tgacggtgat
     5341 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     5401 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5461 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5521 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5581 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5641 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     5701 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5761 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5821 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5881 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5941 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     6001 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     6061 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     6121 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     6181 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     6241 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     6301 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     6361 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     6421 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     6481 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     6541 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6601 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     6661 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     6721 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     6781 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6841 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6901 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6961 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     7021 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7081 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7141 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7201 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7261 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7321 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     7381 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7441 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7501 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7561 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7621 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7681 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7741 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7801 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7861 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7921 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7981 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8041 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8101 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8161 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8221 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8281 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8341 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8401 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8461 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8521 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8581 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     8641 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8701 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8761 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8881 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8941 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9481 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9781 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9841 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9901 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9961 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10021 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10081 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10141 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10201 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10261 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10321 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10381 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10441 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10501 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10561 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10621 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10681 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10741 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10801 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10861 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10921 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10981 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    11041 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11101 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11161 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11221 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    11281 gctg
//
