LOCUS       V026703                11622 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026703
VERSION     V026703
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5641
                     /label="GRIN3A(NM_133445)"
                     /note="GRIN3A(NM_133445)"
                     /gene="GRIN3A"
     CDS             5648..5737
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5781..5992
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6005..6273
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6302..6311
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6332..6994
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7064..7117
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7118..7717
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7718..8305
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8379..8612
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8684..8805
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8824..8959
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8997..9013)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9021..9037
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9045..9075)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9090..9111
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9399..9987)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10158..11018)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11019..11123)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11597..11613
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga ggagactgag tttgtggtgg ctgctgagca gggtctgtct
     2341 gctgttgccg ccgccctgcg cactggtgct ggccggggtg cccagctcct cctcgcaccc
     2401 gcagccctgc cagatcctca agcgcatcgg gcacgcggtg agggtgggcg cggtgcactt
     2461 gcagccctgg accaccgccc cccgcgcggc cagccgcgct ccggacgaca gccgagcagg
     2521 agcccagagg gatgagccgg agccagggac taggcggtcc ccggcgccct cgccgggcgc
     2581 acgctggttg gggagcaccc tgcatggccg ggggccgccg ggctcccgta agcccgggga
     2641 gggcgccagg gcggaggccc tgtggccacg ggacgccctc ctatttgccg tggacaacct
     2701 gaaccgcgtg gaagggctgc taccctacaa cctgtctttg gaagtagtga tggccatcga
     2761 ggcaggcctg ggcgatctgc cacttttgcc cttctcctcc cctagttcgc catggagcag
     2821 tgaccctttc tccttcctgc aaagtgtgtg ccataccgtg gtggtgcaag gggtgtcggc
     2881 gctgctcgcc ttcccccaga gccagggcga aatgatggag ctcgacttgg tcagcttagt
     2941 cctgcacatt ccagtgatca gcatcgtgcg ccacgagttt ccacgggaga gtcagaatcc
     3001 ccttcaccta caactgagtt tagaaaattc attaagttct gatgctgatg tcactgtctc
     3061 aatcctgacc atgaacaact ggtacaattt tagcttgttg ctgtgccagg aagactggaa
     3121 catcaccgac ttcctcctcc ttacccagaa taattccaag ttccaccttg gttctatcat
     3181 caacatcacc gctaacctcc cctccaccca ggacctcttg agcttcctac agatccagct
     3241 tgagagtatt aagaacagca cacccacagt ggtgatgttt ggctgcgaca tggaaagtat
     3301 ccggcggatt ttcgaaatta caacccagtt tggggtcatg ccccctgaac ttcgttgggt
     3361 gctgggagat tcccagaatg tggaggaact gaggacagag ggtctgccct tagggctcat
     3421 tgctcatgga aaaacaacac agtctgtctt tgagcactac gtacaagatg ctatggagct
     3481 ggtcgcaaga gctgtagcca cagccaccat gatccaacca gaacttgctc tcattcccag
     3541 cacgatgaac tgcatggagg tggaaactac aaatctcact tcaggacaat atttatcaag
     3601 gtttctagcc aataccactt tcagaggcct cagtggttcc atcagagtaa aaggttccac
     3661 catcgtcagc tcagaaaaca actttttcat ctggaatctt caacatgacc ccatgggaaa
     3721 gccaatgtgg acccgcttgg gcagctggca ggggggaaag attgtcatgg actatggaat
     3781 atggccagag caggcccaga gacacaaaac ccacttccaa catccaagta agctacactt
     3841 gagagtggtt accctgattg agcatccttt tgtcttcaca agggaggtag atgatgaagg
     3901 cttgtgccct gctggccaac tctgtctaga ccccatgact aatgactctt ccacattgga
     3961 cagccttttt agcagcctcc atagcagtaa tgatacagtg cccattaaat tcaagaagtg
     4021 ctgctatgga tattgcattg atctgctgga aaagatagca gaagacatga actttgactt
     4081 cgacctctat attgtagggg atggaaagta tggagcatgg aaaaatgggc actggactgg
     4141 gctagtgggt gatctcctga gagggactgc ccacatggca gtcacttcct ttagcatcaa
     4201 tactgcacgg agccaggtga tagatttcac cagccctttc ttctccacca gcttgggcat
     4261 cttagtgagg acccgagata cagcagctcc cattggagcc ttcatgtggc cactccactg
     4321 gacaatgtgg ctggggattt ttgtggctct gcacatcact gccgtcttcc tcactctgta
     4381 tgaatggaag agtccatttg gtttgactcc caaggggcga aatagaagta aagtcttctc
     4441 cttttcttca gccttgaaca tctgttatgc cctcttgttt ggcagaacag tggccatcaa
     4501 acctccaaaa tgttggactg gaaggtttct aatgaacctt tgggccattt tctgtatgtt
     4561 ttgcctttcc acatacacgg caaacttggc tgctgtcatg gtaggtgaga agatctatga
     4621 agagctttct ggaatacatg accccaagtt acatcatcct tcccaaggat tccgctttgg
     4681 aactgtccga gaaagcagtg ctgaagatta tgtgagacaa agtttcccag agatgcatga
     4741 atatatgaga aggtacaatg ttccagccac ccctgatgga gtggagtatc tgaagaatga
     4801 tccagagaaa ctagacgcct tcatcatgga caaagccctt ctggattatg aagtgtcaat
     4861 agatgctgac tgcaaacttc tcactgtggg gaagccattt gccatagaag gatacggcat
     4921 tggcctccca cccaactctc cattgaccgc caacatatcc gagctaatca gtcaatacaa
     4981 gtcacatggg tttatggata tgctccatga caagtggtac agggtggttc cctgtggcaa
     5041 gagaagtttt gctgtcacgg agactttgca aatgggcatc aaacacttct ctgggctctt
     5101 tgtgctgctg tgcattggat ttggtctgtc cattttgacc accattggtg agcacatagt
     5161 atacaggctg ctgctaccac gaatcaaaaa caaatccaag ctgcaatact ggctccacac
     5221 cagccagaga ttacacagag caataaatac atcatttata gaggaaaagc agcagcattt
     5281 caagaccaaa cgtgtggaaa agaggtctaa tgtgggaccc cgtcagctta ccgtatggaa
     5341 tacttccaat ctgagtcatg acaaccgacg gaaatacatc tttagtgatg aggaaggaca
     5401 aaaccagctg ggcatccgga tccaccagga catccccctc cctccaagga gaagagagct
     5461 ccctgccttg cggaccacca atgggaaagc agactcccta aatgtatctc ggaactcagt
     5521 gatgcaggaa ctctcagagc tcgagaagca gattcaggtg atccgtcagg agctgcagct
     5581 ggctgtgagc aggaaaacgg agctggagga gtatcaaagg acaagtcgga cttgtgagtc
     5641 cggatccgaa caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga
     5701 agatttagag caaaagttaa tttccgagga ggacttatag cggccgcgaa ggatctgcga
     5761 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5821 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5881 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5941 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     6001 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     6061 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     6121 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     6181 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     6241 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     6301 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     6361 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     6421 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct
     6481 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     6541 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     6601 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat
     6661 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     6721 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     6781 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     6841 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     6901 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     6961 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc
     7021 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct
     7081 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     7141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7321 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7681 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     7741 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7801 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7861 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7921 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7981 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8041 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8101 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     8161 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8221 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     8281 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     8341 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8401 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8461 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8521 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8581 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8641 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8701 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8761 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8821 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8881 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8941 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     9001 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9061 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9121 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9181 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    11161 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    11221 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11281 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    11341 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11401 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    11461 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    11521 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11581 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
