LOCUS       V046094                11413 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046094
VERSION     V046094
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2297..2306
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3380..5560
                     /label="GPD2(NM_000408)"
                     /note="GPD2(NM_000408)"
                     /gene="GPD2"
     promoter        5611..5822
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5835..6103
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6138..6845
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6855..6908
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6909..7508
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7509..8096
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8170..8403
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8475..8596
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8615..8750
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8788..8804)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8812..8828
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8836..8866)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8881..8902
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9190..9778)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9949..10809)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10810..10914)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11388..11404
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggctag caagaaagtc tgcattgtag gctccgggaa
     2341 ctggggctca gccatcgcca agatcgtggg tggcaatgca gcccagctgg cacagtttga
     2401 cccacgggtg accatgtggg tatttgagga agacattgga ggcaaaaagc tgactgagat
     2461 catcaacacg cagcatgaga atgtcaaata cctgccaggg cacaagttgc ccccaaatgt
     2521 ggtggctgtc ccagatgtgg tccaggctgc agaggatgct gacatcctga tctttgtggt
     2581 gccccatcag ttcatcggca agatctgtga ccagctcaag ggccatctga aggcaaacgc
     2641 cactggcata tctcttatta agggggtaga cgagggcccc aatgggctga agctcatctc
     2701 ggaagtgatt ggggagcgcc tcggcatccc catgagtgtg ctgatggggg ccaacattgc
     2761 cagcgaggtg gctgatgaga agttctgtga gacaaccatt ggctgcaagg acccggccca
     2821 gggacaactc ctgaaagagc tgatgcagac accaaacttc cgtatcacag tggtgcaaga
     2881 ggtggacaca gtagagatct gtggagcctt aaagaatgta gtggccgtgg gggctggctt
     2941 ctgtgatggc ctgggctttg gcgacaacac caaggcggca gtgatccggc tgggactcat
     3001 ggagatgata gccttcgcca agctcttctg cagtggccct gtgtcctctg ccaccttctt
     3061 ggagagctgt ggtgttgctg acctgatcac tacctgctat ggagggcgga accggaaagt
     3121 ggctgaggcc tttgcgcgta caggaaagtc cattgagcag ctggagaaag agttgctgaa
     3181 tgggcagaaa ctgcaggggc ccgagacagc ccgggagcta tacagcatcc tccagcacaa
     3241 gggcctggta gacaagtttc ccttgttcat ggctgtgtac aaggtgtgct acgagggcca
     3301 gccagtgggt gaattcatcc actgcctgca gaatcatcca gaacatatgg gtggaggcgg
     3361 atcaggaggc ggtggatcta tggcatttca aaaggcagtg aaagggacga ttcttgttgg
     3421 aggaggtgct cttgcaactg ttttaggact ttctcagttt gctcattaca gaaggaaaca
     3481 aatgaacctg gcctatgtta aagcagcaga ctgcatttca gaaccagtta acagggagcc
     3541 tccttccaga gaagctcagc tactgacttt gcaaaacaca tctgaatttg atatccttgt
     3601 tattggagga ggagcaacag gaagtggctg tgcgctagat gctgtcacca gaggactaaa
     3661 aacagccctt gtagaaagag atgatttctc atcagggacc agcagcagaa gcactaaatt
     3721 gatccatggt ggtgtgagat atctgcagaa ggccatcatg aagttggata ttgagcagta
     3781 taggatggta aaagaagccc ttcatgagcg tgccaacctg ctagaaattg ctccccattt
     3841 atcagctcca ttgcctataa tgcttccagt ttacaagtgg tggcagttac cttactactg
     3901 ggtaggaatc aagctgtatg atttggttgc aggaagcaat tgcctaaaaa gcagttatgt
     3961 cctcagcaaa tcaagagccc ttgaacattt cccaatgctc cagaaggaca aactggtagg
     4021 agcaattgtc tactatgacg gacaacataa cgatgcacgg atgaaccttg ccattgctct
     4081 gactgctgcc aggtatgggg ctgccacagc caattacatg gaggtagtga gcttgctcaa
     4141 gaagacagac ccccagacag ggaaagtgcg tgtgagcggc gcacggtgca aggatgtcct
     4201 cacagggcag gaatttgacg tgagagccaa atgtgttatc aatgccacgg gacctttcac
     4261 ggactctgtg cgcaaaatgg atgataaaga cgcagcagct atctgccagc caagtgctgg
     4321 tgtccatatt gtgatgcctg gttattacag cccagagagc atgggacttc ttgacccagc
     4381 gaccagtgat gggcgagtta ttttcttctt accctggcaa aagatgacga tcgctggcac
     4441 tactgatact ccaactgatg ttacacacca tccaattcct tcagaagaag atatcaactt
     4501 cattttgaat gaagtgcgta attacctgag ttgtgatgtt gaagtgagaa gaggggatgt
     4561 cctggcagca tggagtggaa tccgtcctct tgttacagac cccaaatctg cagatactca
     4621 gtctatctcc cgaaatcatg ttgttgatat cagtgagagt ggccttatta ctatagcagg
     4681 tggaaagtgg acaacttatc ggtctatggc agaagatacc ataaatgctg ctgtcaaaac
     4741 tcataattta aaagcaggac caagtagaac agttgggctt ttccttcaag ggggtaaaga
     4801 ttggagcccc acactctaca ttaggcttgt gcaggattat ggacttgaaa gcgaggtggc
     4861 acagcatctt gccgccacct atggtgataa ggcctttgag gtggccaaaa tggcaagtgt
     4921 gactggcaaa aggtggccta ttgttggagt acgtcttgtg tcagaatttc catatattga
     4981 agcagaggtg aaatatggga ttaaggagta tgcctgcact gctgtggata tgatttcacg
     5041 tcgtactcgc ctggcctttc taaatgtcca ggcagcagag gaagccctac ccaggattgt
     5101 tgaactgatg ggcagggaac tgaattggga tgattataag aagcaggaac aacttgaaac
     5161 agccaggaag tttctatatt atgaaatggg ctataaatct cgatcagaac agttaacaga
     5221 tcgctctgaa attagcctac tgccttcaga cattgacagg tataagaaga gatttcataa
     5281 gtttgatgca gaccagaaag gctttattac cattgttgat gttcagcgtg tattagagag
     5341 tatcaatgtc caaatggatg aaaatacact ccatgaaatt ctaaatgaag ttgatttgaa
     5401 taaaaatgga caggttgaac tcaatgaatt tttgcagctg atgagtgcta ttcaaaaagg
     5461 aagggtatct ggaagccggc ttgctatact aatgaaaact gcagaagaga acctcgacag
     5521 aagagttcca attccagtgg accgtagttg tggaggattg tgaggatccg cggccgcgaa
     5581 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     5641 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     5701 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     5761 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     5821 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     5881 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     5941 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     6001 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     6061 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     6121 ctacgctaga cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     6181 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6301 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6481 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6661 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6721 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6781 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6841 acaagggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     6901 ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6961 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7021 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7081 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7141 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7201 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7261 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7321 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7381 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7441 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7501 gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7561 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7621 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7681 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7741 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7801 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7861 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     7921 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7981 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8041 cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg
     8101 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8161 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     8221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8461 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8581 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     8641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8701 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     8761 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8821 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8881 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8941 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9001 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9061 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9601 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9841 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9901 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10081 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10261 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10501 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10801 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10861 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10921 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10981 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    11041 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    11101 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    11161 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11221 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11281 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11341 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11401 cagtgccaag ctg
//
