LOCUS       V035814                10205 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035814
VERSION     V035814
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4308
                     /label="GLS(NM_014905)"
                     /note="GLS(NM_014905)"
                     /gene="GLS"
     CDS             4315..4341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4345..4371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4375..4401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4408..5127
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5175..5386
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5399..5667
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5694..6293
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6300..6888
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6962..7195
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7267..7388
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7407..7542
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7580..7596)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7604..7620
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7628..7658)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7673..7694
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7982..8570)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8741..9601)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9602..9706)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10180..10196
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatgcgg ctgcgaggct cggggatgct gcgggacctg
     2341 ctcctgcggt cgcccgccgg cgtgagcgcg actctgcggc gggcacagcc cttggtcacc
     2401 ctgtgccggc gtccccgagg cgggggacgg ccggccgcgg gcccggctgc cgccgcgcga
     2461 ctccacccgt ggtggggcgg gggcggctgg ccggcggagc ccctcgcgcg gggcctgtcc
     2521 agctctcctt cggagatctt gcaggagctg ggcaagggga gcacgcatcc gcagcccggg
     2581 gtgtcgccac ccgctgcccc ggcggcgccc ggccccaagg acggccccgg ggagacggac
     2641 gcgtttggca acagcgaggg caaagagctg gtggcctcag gtgaaaataa aataaaacag
     2701 ggtctgttac ctagcttgga agatttgctg ttctatacaa ttgctgaagg acaagagaaa
     2761 atacctgttc ataaatttat tacagcactc aaatctacag gattgcgaac gtctgatccc
     2821 aggttgaaag agtgtatgga tatgttaaga ttaactcttc aaacaacatc agatggtgtc
     2881 atgctagaca aagatctttt taaaaaatgt gttcagagca acattgtttt gttgacacaa
     2941 gcatttagaa gaaagtttgt gattcctgac tttatgtctt ttacctcaca cattgatgag
     3001 ttatatgaaa gtgctaaaaa gcagtctgga ggaaaggttg cagattatat tcctcaactg
     3061 gccaaattca gtcccgattt gtggggtgtg tctgtttgta cagtagatgg acagaggcat
     3121 tctactggag ataccaaagt tcccttctgt cttcagtcct gtgtaaaacc tttgaaatat
     3181 gccattgctg ttaatgatct tggaactgaa tatgtgcatc gatatgttgg aaaagagccg
     3241 agtggactaa gattcaacaa actatttttg aatgaagatg ataaaccaca taatcctatg
     3301 gtaaatgctg gagcaattgt tgtgacttca ctaataaagc aaggagtaaa taatgctgaa
     3361 aaatttgact atgtcatgca gtttttgaat aagatggctg gtaatgaata tgttggattc
     3421 agtaatgcaa cgtttcagtc tgaaagagaa agtggagatc gaaattttgc aataggatat
     3481 tacttaaaag aaaagaagtg ttttccagaa ggcacagaca tggttggtat attagacttc
     3541 tacttccagc tgtgctccat tgaagtgact tgtgaatcag ccagtgtgat ggctgcgaca
     3601 ctggctaatg gtggtttctg cccaattact ggtgaaagag tactgagccc tgaagcagtt
     3661 cgaaatacat tgagtttgat gcattcctgt ggcatgtatg acttctcagg gcagtttgct
     3721 ttccatgttg gtcttcctgc aaaatctgga gttgctgggg gcattctttt agttgtcccc
     3781 aatgttatgg gtatgatgtg ctggtctcct cctctggata agatgggcaa cagtgttaag
     3841 ggaattcact tttgtcacga tcttgtttct ctgtgtaatt tccataacta tgataatttg
     3901 agacactttg caaaaaaact tgatcctcga agagaaggtg gtgatcaaag ggtaaagtca
     3961 gtgataaatc ttttgtttgc tgcatatact ggagatgtgt ctgcacttcg aagatttgct
     4021 ttgtcagcta tggacatgga acagcgggac tatgattcta gaacagcact ccatgtagct
     4081 gctgcagagg gtcatgttga agttgttaaa tttttgctgg aagcctgcaa agtaaaccct
     4141 ttccccaagg acaggtggaa taacactccc atggatgaag cactgcactt tggacaccat
     4201 gatgtattta aaattctcca agaataccaa gtccagtaca cacctcaagg agattctgac
     4261 aacgggaagg aaaatcaaac cgtccataag aatcttgatg gattgttggg atcctaccca
     4321 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct
     4381 tatgatgtcc cagactacgc agccaccatg gtgagcaagg gcgaggagct gttcaccggg
     4441 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4501 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4561 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4621 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4681 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4741 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4801 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4861 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4921 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4981 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5041 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5101 ctcggcatgg acgagctgta caagtaggga tccgcggccg cgaaggatct gcgatcgctc
     5161 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5221 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5281 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5341 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     5401 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     5461 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     5521 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     5581 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     5641 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg
     5701 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     5761 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     5821 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5881 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5941 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6001 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6061 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6121 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6181 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6241 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca
     6301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6661 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     6901 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6961 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7021 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7081 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7141 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7201 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7261 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7381 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     7441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     7561 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7621 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7681 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7741 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8401 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8641 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9061 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9121 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9181 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9241 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9301 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9361 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9421 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9481 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9541 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9601 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9661 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9721 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9781 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     9841 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     9901 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     9961 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10021 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10081 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    10141 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    10201 agctg
//
